Compositional Protein Analysis Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, a Novel Derivatization Reagent

Cohen, Steven A.; DeAntonis, Kathryn; Michaud, Dennis P.

doi:10.1016/b978-0-12-058757-5.50036-6

Cited by 33 publications

(25 citation statements)

References 2 publications

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“…Filtered samples were then derivatized at 55°C with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. 13 Dual pumps (Waters 510) were used to deliver the binary mobile phase (140 mmol/L of sodium acetate, with 17 mmol/L of triethylamine (TEA) titrated to pH 5.05, and 60% acetonitrile) at 1 mL/min using the AccQ Tag gradient table to a C18 column (Nova-Pak, 4μm, 3.9 × 150 mm; Waters) that was maintained at 60°C. Ten-microliter samples were injected by autosampler (Waters 717 plus) with the eluting products measured by a fluorescence detector (Waters 474) fit with a 5-μL flow cell, at excitation and emission wave lengths of 250 and 395 nm (gain, 100), respectively.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

Indices of activity of the nitric oxide system in hemodialysis patients

Schmidt

Domico

Samsell

et al. 1999

American Journal of Kidney Diseases

102

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Arginine deficiency and/or increased levels of circulating nitric oxide (NO) synthesis (NOS) inhibitors can cause reduced NOS, which may contribute to hypertension in patients with end-stage renal disease (ESRD). To test these hypotheses, NO oxidation products (NO 2 + NO 3 = NO x ) and cyclic guanosine monophosphate (cGMP), the vasodilatory second messenger of NO, were measured in the blood, urine, and dialysate effluent of hemodialysis (HD) patients and compared with the blood and urine of healthy subjects. The subjects ate a controlled low-nitrate diet (∼330 μmol/d) for 48 hours before and during blood, dialysis effluent, and 24-hour urine collection. NO x output was significantly reduced in HD patients versus controls (552 ± 51 v 824 ± 96 μmol/24 h; P < 0.001), whereas cGMP output was not low versus controls. Plasma arginine level was normal and plasma levels of citrulline and the endogenous NOS inhibitor, asymmetric dimethylarginine (ADMA), were markedly elevated in patients with ESRD versus controls. Systolic blood pressure was greater in HD patients compared with controls despite concurrent antihypertensive therapy in most patients with ESRD. These studies suggest NO production is low in patients with ESRD undergoing HD, possibly because of the increased ratio of plasma ADMA to arginine. Index WordsEnd-stage renal disease; hypertension; arginine; cyclic guanosine monophosphate (cGMP); endogenous nitric oxide synthases (NOS) inhibitors Nitric oxide (NO) is a potent vasodilator made from L-arginine by the enzymatic action of several widely distributed nitric oxide synthases (NOSs). The constitutively expressed, vascular endothelial and brain-type NOSs have major roles in the physiological control of vascular tone and kidney function, and many of these actions are mediated by cyclic guanosine monophosphate (cGMP). 1,2 NO deficiency can be produced experimentally by the administration of substituted L-arginine analogues that function as competitive inhibitors of NOS. Chronic experimental NOS inhibition in animals produces hypertension, and NOS may be defective in some individuals with primary and secondary hypertension. 3 Hypertension is particularly prevalent in hemodialysis (HD) patients with end-stage renal disease (ESRD), 4-7 and there are valid reasons to suspect NO deficiency might occur and contribute to the hypertension in this population. We previously reported that patients with ESRD undergoing peritoneal dialysis (PD) produce less NO, indicated by the removal rate of the stable inert oxidation products of NO (NO 2 In addition, the plasma levels of naturally occurring compounds that inhibit the L-arginine-NO pathway accumulate in patients with ESRD. For example, plasma levels of asymmetric dimethylarginine (ADMA) increase in HD patients, possibly to concentrations that inhibit NOS, although this remains controversial. 9,10 This, together with plasma arginine levels in the low-normal range, 9,11 means the ratio of plasma levels of endogenous NOS inhibitors to Larginine is high in patients wit...

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Section: Sample Collection and Preparationmentioning

confidence: 99%

Indices of activity of the nitric oxide system in hemodialysis patients

Schmidt

Domico

Samsell

et al. 1999

American Journal of Kidney Diseases

102

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“…For glutathione analysis, tissue samples were homogenized in pH 7.4 phosphate buffer containing EDTA, acivicin, and monobromobimane. Neutralized perchloric acid extracts of tumor tissue were analyzed by highperformance liquid chromatography for glutathione (7) or glycine (9). Both analytes were normalized to tissue wet weight.…”

Section: Methodsmentioning

confidence: 99%

“…A fitted infusion harness and lines allowed free movement throughout the cage. Sterile solutions of [2][3][4][5][6][7][8][9][10][11][12][13] C]glycine were prepared in saline and infused at a rate of 0.5 or 1.0 mmol/kg/h (0.5 mL/h).…”

Section: Introductionmentioning

confidence: 99%

Noninvasive In vivo Detection of Glutathione Metabolism in Tumors

Thelwall

Yemin

Gillian³

et al. 2005

Cancer Research

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Magnetic resonance spectroscopic imaging has been used to follow glutathione metabolism and evaluate glutathione heterogeneity in intact tumor tissue. Stable isotope-labeled glutathione was detected in s.c. implanted fibrosarcoma tumors in anesthetized rats following infusion of [2-13 C]glycine. Using 1 H-decoupled 13 C magnetic resonance spectroscopy, the appearance of [2-13 C]glycine at 42.4 ppm and the subsequent incorporation of this isotope label into the glycyl residue of glutathione at 44.2 ppm can be detected. The identity and relative concentrations of labeled metabolites observed in the in vivo spectrum were confirmed in studies of tissue extracts. The high level of isotopic enrichment and the concentration of glutathione in tumor tissue allow for collection of spatially localized spectra using 13 C chemical shift imaging methods. These data provide the first direct images of glutathione in intact tumor tissue and show metabolic heterogeneity. This method may lead to the ability to monitor changes in tumor tissue redox state that may ultimately affect diagnosis, monitoring, and treatment. (Cancer Res 2005; 65(22): 10149-53)

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“…The HPLC-purified bioactive fraction (coronamycin) was dissolved in 50 % (v/v) methanol in water and subjected to hydrolysis by HCl according to previously described methods (Castillo et al, 2002). Samples then were analysed by precolumn 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization followed by reverse-phase HPLC (Cohen & Michaud, 1993). An Alliance/Millenium HPLC system (Waters) equipped with an AccQTag amino acid analysis column was used.…”

Section: Methodsmentioning

confidence: 99%

Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.

et al. 2004

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Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans. It is also active against the malarial parasite, Plasmodium falciparum, with an IC 50 of 9?0 ng ml "1. Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217?9 and 1203?8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2 : 2 : 1 : 1 : 3. Other compounds with antifungal activities are also produced by this endophytic streptomycete.

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Compositional Protein Analysis Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, a Novel Derivatization Reagent

Cited by 33 publications

References 2 publications

Indices of activity of the nitric oxide system in hemodialysis patients

Indices of activity of the nitric oxide system in hemodialysis patients

Noninvasive In vivo Detection of Glutathione Metabolism in Tumors

Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.

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