Compositional Changes in Cell Wall Polysaccharides from Japanese Plum (Prunus salicina Lindl.) during Growth and On-Tree Ripening

Abstract

Climacteric Japanese plums were harvested at six developmental stages with no intermediate storage period, and cell wall compositional changes were analyzed. Arabinose proved to be the principal neutral monosaccharide constituent in cell walls during growth and the most dynamic neutral sugar in pectic fractions. Arabinose loss from tightly bound pectins was found to be a relatively early feature in the sequence of cell wall biochemical modifications, thus suggesting a softening-related role during Japanese plum on-tree ripening. Depolymerization of matrix glycans started at the end of the cell expansion phase and increased throughout ripening. Pectin solubilization was first detected during early ripening. Firmness loss did not correlate with polyuronide depolymerization early in ripening, but the last softening phase was associated with a strong depolymerization of cell wall polyuronides as well as a decrease in the arabinose/galactose ratio in loosely bound pectins. This is the first work that characterizes the temporal sequence of cell wall polysaccharide changes in Japanese plum.

Comparative study of emulsifying properties in acidic condition of soluble polysaccharides fractions obtained from soy hull and defatted soy flour

“…3 mg) was hydrolyzed with 2 M trifluoroacetic acid (TFA, 1 mL) for 90 min at 120°C in closed-cap vials. The TFA was eliminated by evaporation, and the resulting monosaccharides were reduced to alditols using NaBH 4 and converted to alditol acetates to determine the neutral sugar composition of both fractions (Ponce et al 2010). Consequently, these compounds were analyzed by using a Hewlett-Packard 5890 gas chromatograph (Agilent Technologies, Inc.) fitted with a capillary column 30 m × 0.25 mm i.d.…”

Compositional Changes in ‘Bartlett’ Pear (Pyrus communis L.) Cell Wall Polysaccharides As Affected by Sunlight Conditions

“…Decreases in AIR content have reported during ripening of various fruits. 8,9 As pear ripening progresses, extensive loss of arabinose from cell wall polysaccharides occurs. 10,11 The hydrolytic cleavage of arabinose would yield sugar residues that are readily soluble in ethanol during the retrieval of the AIR.…”

“…Polyuronides are usually extracted in water, CDTA, or Na 2 CO 3 , the fractions representing loosely, ionically, or tightly bound polymers, 8 respectively. However, some UA could remain associated with other wall components and be extracted only in harsher media.…”

“…For this purpose, the phenolÀH 2 SO 4 reaction was also carried out with a galacturonic acid standard, which showed an absorbance ratio of 0.28 against the same glucose weight. 8 Size Exclusion Chromatography (SEC). To examine the size distributions of polymers in CDTA-F and Na 2 CO 3 -F, ca.…”

Compositional Changes in Cell Wall Polysaccharides from Five Sweet Cherry (Prunus avium L.) Cultivars during On-Tree Ripening

“…Firm cultivars showed upon ripening a lower neutral sugars/uronic 14 acid (NS/UA) ratio in the NSF suggesting that they have a lower proportion of highly 15 branched polyuronides. The similar molar ratios of arabinose plus galactose to 16 rhamnose [(Ara+Gal)/Rha] suggest that the cultivars differed in their relative 17 proportion of homogalacturonan (HG) and rhamnogalacturonan I (RG-I) rather than in 18 the size of the RG side chains; with greater proportions of HG in firmer cherries. 26 Cherry fruit exports to Asia and other emerging markets have rapidly grown in recent 27 years.…”