Compositional and functional profiling of the rhizosphere microbiomes of the invasive weed <i>Ageratina adenophora</i> and native plants

Xia, Yun; Dong, Mingzhe; Yu, Li; Kong, Lingyin; Seviour, Robert J.; Kong, Yunhong

doi:10.7717/peerj.10844

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…Du et al 10.3389/fmicb.2023.1131797 Frontiers in Microbiology 10 frontiersin.org by significantly enhancing the N and P of the former. Therefore, AMFcan enhance A. adenophora's competitiveness by providing more nutrients, which may be attributed to their different AMF colonization rates (Waller et al, 2016;Xia et al, 2021). Compared to monoculture treatment, the AMF colonization rate of R. amethystoides was significantly decreased, while the AMF colonization rate of A. adenophora was significantly increased when the two plant species were in interspecific competition.…”

Section: Discussionmentioning

confidence: 99%

Effects of Septoglomus constrictum and Bacillus cereus on the competitive growth of Ageratina adenophora

Chen

Yang

et al. 2023

Front. Microbiol.

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Beneficial microorganisms play a pivotal role in the invasion process of exotic plants, including arbuscular mycorrhizal fungi (AMF) and Bacillus. However, limited research exists on the synergistic influence of AMF and Bacillus on the competition between both invasive and native plants. In this study, pot cultures of Ageratina adenophora monoculture, Rabdosia amethystoides monoculture, and A. adenophora and R. amethystoides mixture were used to investigate the effects of dominant AMF (Septoglomus constrictum, SC) and Bacillus cereus (BC), and the co-inoculation of BC and SC on the competitive growth of A. adenophora. The results showed that inoculation with BC, SC, and BC + SC significantly increased the biomass of A. adenophora by 14.77, 112.07, and 197.74%, respectively, in the competitive growth between A. adenophora and R. amethystoides. Additionally, inoculation with BC increased the biomass of R. amethystoides by 185.07%, while inoculation with SC or BC + SC decreased R. amethystoides biomass by 37.31 and 59.70% compared to the uninoculated treatment. Inoculation with BC significantly increased the nutrient contents in the rhizosphere soil of both plants and promoted their growth. Inoculation with SC or SC + BC notably increased the nitrogen and phosphorus contents of A. adenophora, therefore enhancing its competitiveness. Compared with single inoculation, dual inoculation with SC and BC increased AMF colonization rate and Bacillus density, indicating that SC and BC can form a synergistic effect to further enhance the growth and competitiveness of A. adenophora. This study reveals the distinct role of S. constrictum and B. cereus during the invasion of A. adenophora, and provide new clues to the underlying mechanisms of interaction between invasive plant, AMF and Bacillus.

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Section: Discussionmentioning

confidence: 99%

Effects of Septoglomus constrictum and Bacillus cereus on the competitive growth of Ageratina adenophora

Chen

Yang

et al. 2023

Front. Microbiol.

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“…aquimarina , were found in rhizospheric soil of A. adenophora ( Figure 1 ). The invasion of A. adenophora selected specific microbial taxa in soil, which may have mediated soil nutrient cycling and thus potentially improved plant nutrient acquisition ( Xia et al, 2021 ; Li et al, 2022 ). Different Bacillus taxa vary in their ability to supply plants with nutrients ( Pinyapach et al, 2018 ; Kang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, A. adenophora invasion leads to a decrease in actinomycetes but increases in aerobic, anaerobic bacteria, and nitrogen-fixing bacteria ( Niu et al, 2007 ; Xu et al, 2012 ; Fang et al, 2019 ). The rhizospheric soil microbiomes of A. adenophora differed to varying degrees in the relative abundances of bacterial and fungal phyla and genera from those of two native plants, Artemisia indica and Imperata cylindrica , and were more metabolically active than both of these, as indicated by marked increases in the expression levels of genes associated with the cell wall, cell membrane, and envelope biogenesis, energy production and conversion, and the transport of carbohydrates, amino acids, coenzymes, nucleotides, and secondary metabolites ( Xia et al, 2021 ). Previous studies have reported that A. adenophora can result in the accumulation of Bacillus , such as B. megaterium and B. cereus , which might significantly promote the growth of A. adenophora compared to native plants ( Niu et al, 2007 ; Sun et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Rhizospheric Bacillus-Facilitated Effects on the Growth and Competitive Ability of the Invasive Plant Ageratina adenophora

Chen

et al. 2022

Front. Plant Sci.

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The rhizospheric microbial community affects the population establishment of invasive plants in introduced areas, among which Bacillus has numerous functions in promoting plant growth. This study isolated and enriched the Bacillus community in the rhizospheric soil of the invasive plant Ageratina adenophora and the native accompanying plant Rabdosia amethystoides. The effects of these rhizospheric Bacillus communities on the growth and competition of A. adenophora and R. amethystoides were evaluated in pot experiments. The results showed that the number and diversity of Bacillus in the rhizospheric soil of A. adenophora were higher than those of R. amethystoides (A. adenophora: 122 strains in soil, 16 Bacillus taxa; R. amethystoides: 88 strains in soil, 9 Bacillus taxa). After Bacillus inoculation of A. adenophora in a pot experiment, Bacillus idriensis, Bacillus toyonensis and Bacillus cereus were accumulated in the rhizospheric of A. adenophora, which significantly increased the nitrate nitrogen (NO3–-N) content in the soil and the total carbon and nitrogen concentrations in A. adenophora in the mixed treatment. The selective accumulation of Bacillus enhanced the competitive advantage of A. adenophora over the native accompanying plant; the corrected index of relative competition intensity of A. adenophora-inoculated Bacillus reached double that of the uninoculated treatment, and the growth of native plants was greatly suppressed under mixed planting. Our study confirmed that invasion of A. adenophora can lead to the accumulation of specific Bacillus taxa in the rhizospheric soil, which in turn can increase the competitive advantage of A. adenophora.

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“…It is known that in some species, such as Crotalaria, nodulation is required for alkaloid production [7]. Functional analysis of soil metagenomic data has expanded as a field, and is used as a way to consider how plants are influencing the microbiome [8]. The microbiome is equally important in understanding plant secondary metabolism.…”

Section: The Significance Of the Plant Microbiomementioning

confidence: 99%

Microbes in the <em>Datura</em> Rootzone Contribute to an Antioxidant Support System of Flavonoids and Other Aromatic Compounds

Senn¹,

Pangell²,

Bowerman³

2021

Preprint

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The purpose of this paper is to elucidate the roles that microbes may be playing in the rootzone of the medicinal plant Datura inoxia. We hypothesized that rhizospheric and endophytic microbes would be found that were capable of performing the same secondary metabolic functions of the plant rootzone they inhabited. We also hypothesized that the microbial functions would be co-operative with and supportive to plant secondary metabolite production, for example, by providing precursors to important plant bioactive molecules. The methods employed were mi-crobial barcoding, tests of essential oils against antibiotic resistant bacteria and other soil bacterial isolates, 16S Next Generation Sequencing (NGS) metabarcoding, and Whole Genome Shotgun (WGS) taxonomic and functional. A few of the main bacterial genera of interest that were dis-covered in the Datura root microbiome were Flavobacterium, Chitinophaga, Pseudomonas, Strepto-myces, Rhizobium, and Bacillus. In the context of known interactions, and current results, plants and microbes influence the flavonoid biosynthetic pathways of one other, in terms of the regulation of the phenylpropanoid pathway. This is important because these compounds are phyto-protective antioxidants and are precursors to many aromatic bioactive compounds that are relevant to human health. There was strong evidence to support the notion that synergistic production of plant de-rived secondary metabolites by microbes occurred, as well as the ability for the compounds to enter plant cells. There are possible biopharmaceutical and agricultural applications of the natural interplay that was discovered during this study of the Datura inoxia rhizosphere.

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Compositional and functional profiling of the rhizosphere microbiomes of the invasive weed Ageratina adenophora and native plants

Cited by 7 publications

References 34 publications

Effects of Septoglomus constrictum and Bacillus cereus on the competitive growth of Ageratina adenophora

Effects of Septoglomus constrictum and Bacillus cereus on the competitive growth of Ageratina adenophora

Rhizospheric Bacillus-Facilitated Effects on the Growth and Competitive Ability of the Invasive Plant Ageratina adenophora

Microbes in the <em>Datura</em> Rootzone Contribute to an Antioxidant Support System of Flavonoids and Other Aromatic Compounds

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