Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study

Pál, Domonkos; Tóth, Gábor; Sugár, Simon; Fügedi, Kata Dorina; Szabó, Dániel; Kovalszky, Ilona; Papp, Dávid; Schlosser, Gitta; Tóth, Csaba; Tornóczky, Tamás; Drahos, László; Turiák, Lilla

doi:10.3390/ijms24087050

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…Фібринолітична система бере участь у багатьох фізіологічних функціях, серед яких важливі компоненти можуть взаємодіяти один з одним або синергетично, або антагоністично в патогенезі багатьох захворювань. Крім того, не останню роль відіграє підвищення фактора фон Віллебранда в плазмі, посилена активація тромбоцитів і підвищення рівня циркулюючого PAI-1 [13,19].…”

Section: примітка * -р < 005 -вірогідність змін показників хворих пор...unclassified

Clinical manifestations and indicators of the hemostasis system in patients with nonalcoholic fatty liver disease with an immune response to SARS-CoV-2

Didenko,

Yagmur,

Klenina

et al. 2023

GASTRO

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Background. Nonalcoholic fatty liver disease (NAFLD) is often accompanied by comorbid conditions such as obesity, type 2 diabetes, and cardiovascular diseases, which are risk factors for severe coronavirus disease (COVID-19). The latter is characterized by respiratory failure and hyperinflammation with the risk of further multiorgan failure, and disorders in the hemostasis system. Purpose: to determine the clinical manifestations and features of hemostasis in NAFLD in patients with an immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Materials and methods. Thirty-seven patients with NAFLD were under observation, and immunoglobulins G to SARS-CoV-2 were detected in them. Group I consisted of 19 patients who did not suffer from COVID-19. Group II included 18 patients with COVID-19 confirmed by a polymerase chain reaction. Patients of groups I and II were vaccinated against coronavirus. Clinical symptoms, the state of hemostasis in the vascular-platelet link, the state of coagulation, the content of fibrinogen and plasminogen activator inhibitor-1 (PAI-1) were assessed in all patients. Results. Patients with NAFLD and an immune response to SARS-CoV-2 reported a pain syndrome in 70.3 % of cases: group I more often complained of pain in the right (68.4 %) and left (52.6 %) hypochondria, group II — of epigastric pain (66.7 %). Among manifestations of dyspeptic syndrome observed in 28 (75.7 %) patients, in group I complaints of abdominal distension (31.6 %) and stomach rumble (21.1 %) prevailed, in group II — of abdominal distension (50.0 %) and bitter taste in mouth (27.8 %). Concomitant cardiovascular pathology was detected in 32.4 % of cases. The synthetic function of the liver in terms of coagulation factors was almost preserved, but a probable increase in the content of coagulation factor II was observed in patients of group I (p < 0.05). PAI-1 level correlated with prothrombin content (r = 0.365; p = 0.024) and platelet count (r = 0.368; p = 0.023). Conclusions. Patients with an immune response to SARS-CoV-2 in NAFLD had the peculiarities of the clinical picture. In particular, those who did not suffer from COVID-19 complained of pain in the right hypochondrium 1.5 times more often and noted stomach rumble 2 times more often; also, in the hemostasis system, a tendency to increase the number of platelets was revealed compared to the controls (p > 0.05). Meanwhile, among patients with COVID-19, abdominal bloating (by 1.6 times) and bitter taste in mouth (by 2 times) prevailed, and in the hemostasis system, a decrease in the number of platelets was noted compared to the controls (p > 0.05). Patients of both groups had an increase in the average number of platelets by 10 % (p > 0.05). A significant increase by 20 % (p < 0.05) was noted in the content of coagulation factor II and by 12 % in the level of PAI-1 in group I. Correlations of PAI-1 with indicators of the platelet link indicate its participation in the functioning of the homeostasis system.

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Section: примітка * -р < 005 -вірогідність змін показників хворих пор...unclassified

Clinical manifestations and indicators of the hemostasis system in patients with nonalcoholic fatty liver disease with an immune response to SARS-CoV-2

Didenko,

Yagmur,

Klenina

et al. 2023

GASTRO

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“…To date, only proteogenomic analysis of a few ALK gene rearrangement cases has been reported in the literature 24 , and detailed proteomic characterization of tumors carrying this gene alteration has not been performed. Despite the fact that GAG-omic analysis of tissues is still an emerging field, the role of PGs and GAGs, and the alteration of sulfation pattern have been described in many cancer types, including lung cancer 25 – 29 . As the composition of the proteome and glycome are strongly interrelated with each other, performing integrated proteomics and GAG-omics may help to map the ECM- and signaling-related changes in detail.…”

Section: Introductionmentioning

confidence: 99%

Inter- and intratumoral proteomics and glycosaminoglycan characterization of ALK rearranged lung adenocarcinoma tissues: a pilot study

Balbisi

Sugár

Schlosser

et al. 2023

Sci Rep

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Lung cancer is one of the most common types of cancer with limited therapeutic options, therefore a detailed understanding of the underlying molecular changes is of utmost importance. In this pilot study, we investigated the proteomic and glycosaminoglycan (GAG) profile of ALK rearranged lung tumor tissue regions based on the morphological classification, mucin and stromal content. Principal component analysis and hierarchical clustering revealed that both the proteomic and GAG-omic profiles are highly dependent on mucin content and to a lesser extent on morphology. We found that differentially expressed proteins between morphologically different tumor types are primarily involved in the regulation of protein synthesis, whereas those between adjacent normal and different tumor regions take part in several other biological processes (e.g. extracellular matrix organization, oxidation–reduction processes, protein folding) as well. The total amount and the sulfation profile of heparan sulfate and chondroitin sulfate showed small differences based on morphology and larger differences based on mucin content of the tumor, while an increase was observed in both the total amount and the average rate of sulfation in tumors compared to adjacent normal regions.

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Sweet but Challenging: Tackling the Complexity of GAGs with Engineered Tailor‐Made Biomaterials

Le Pennec,

Picart,

Vivès

et al. 2023

Advanced Materials

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Glycosaminoglycans (GAGs) play a crucial role in tissue homeostasis by regulating the activity and diffusion of bioactive molecules. Incorporating GAGs into biomaterials has emerged as a widely adopted strategy in medical applications, owing to their biocompatibility and ability to control the release of bioactive molecules. Nevertheless, immobilized GAGs on biomaterials can elicit distinct cellular responses compared to their soluble forms, underscoring the need to understand the interactions between GAG and bioactive molecules within engineered functional biomaterials. By controlling critical parameters such as GAG type, density, and sulfation, it becomes possible to precisely delineate GAG functions within a biomaterial context and to better mimic specific tissue properties, enabling tailored design of GAG‐based biomaterials for specific medical applications. However, this requires access to pure and well‐characterized GAG compounds, which remains challenging. This review focuses on different strategies for producing well‐defined GAGs and explores high‐throughput approaches employed to investigate GAG–growth factor interactions and to quantify cellular responses on GAG‐based biomaterials. These automated methods hold considerable promise for improving the understanding of the diverse functions of GAGs. In perspective, the scientific community is encouraged to adopt a rational approach in designing GAG‐based biomaterials, taking into account the in vivo properties of the targeted tissue for medical applications.

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Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types—A Pilot Study

Cited by 6 publications

References 39 publications

Clinical manifestations and indicators of the hemostasis system in patients with nonalcoholic fatty liver disease with an immune response to SARS-CoV-2

Clinical manifestations and indicators of the hemostasis system in patients with nonalcoholic fatty liver disease with an immune response to SARS-CoV-2

Inter- and intratumoral proteomics and glycosaminoglycan characterization of ALK rearranged lung adenocarcinoma tissues: a pilot study

Sweet but Challenging: Tackling the Complexity of GAGs with Engineered Tailor‐Made Biomaterials

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