Compositional alterations of gut microbiota in children with primary nephrotic syndrome after initial therapy

Kang, Youjeong; Feng, Dan; Law, Helen Ka-wai; Qu, Wei; Wu, Ying; Zhu, Guoding; Huang, Wenyan

doi:10.1186/s12882-019-1615-4

Cited by 32 publications

(32 citation statements)

References 37 publications

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“…Tsuji et al [ 7 ] further found that pediatric patients in the frequently relapsing INS showed a significant reduction in the proportion of 27 genera ( Propionibacterium , Porphyromonas , Odoribacter , Alistipes , Anaerofustis , Clostridium , Pseudoramibacter , Anaerostipes , Eubacterium , Coprococcus , Butyrivibrio , Lachnoanaerobaculum , Shuttleworthia , Roseburia , Peptoniphilus , Anaerococcus , Anaerotruncus , Peptoclostridium , Faecalibacterium , Holdemanella , Subdoligranulum , Acetonema , Acidaminococcus , Fusobacterium , Megasphaera , Brachyspira , and Treponema ) to which butyrate-producing bacteria belong. Moreover, 4-week initial therapy, including prednisone and compound of vitamin D3 and calcium, could increase the proportion of short-chain fatty acid- (SCFAs-) producing bacteria including Romboutsia , Stomatobaculum , and Cloacibacillus [ 12 ]. Zhang et al [ 8 ] reported the dysbiosis of intestinal microbiome in adult MN.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Profile in Adult Patients with Idiopathic Nephrotic Syndrome

Lin

You

et al. 2021

BioMed Research International

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Background. Increasing evidences have reported gut microbiota dysbiosis in many diseases, including chronic kidney disease and pediatric idiopathic nephrotic syndrome (INS). There is lack evidence of intestinal microbiota dysbiosis in adults with INS, however. Here, we to address the association between the gut microbiome and INS. Methods. Stool samples of 35 adult INS patients and 35 healthy volunteers were collected. Total bacterial DNA was extracted, and the V4 regions of the bacterial 16S ribosomal RNA gene were sequenced. The fecal microbiome was analyzed using bioinformatics. The correlation analysis between altered taxa and clinical parameters was also included. Results. We found that microbial diversity in the gut was reduced in adult patients with INS. Acidobacteria, Negativicutes, Selenomonadales, Veillonellaceae, Clostridiaceae, Dialister, Rombousia, Ruminiclostridium, Lachnospira, Alloprevotella, Clostridium sensu stricto, Megamonas, and Phascolarctobacterium were significantly reduced, while Pasteurellales, Parabacteroides, Bilophila, Enterococcus, Eubacterium ventriosum, and Lachnoclostridium were markedly increased in patients with INS. In addition, Burkholderiales, Alcaligenaceae, and Barnesiella were negatively correlated with serum creatinine. Blood urea nitrogen levels were positively correlated with Christensenellaceae, Bacteroidales_S24.7, Ruminococcaceae, Ruminococcus, and Lachnospiraceae_NK4A136, but were negatively correlated with Flavonifractor_plautii and Erysipelatoclostridium_ramosum. Enterobacteriales, Enterobacteriaceae, Porphyromonadaceae, Escherichia/Shigella, Parabacteroides, and Escherichia_coli were positively correlated with albumin. Proteinuria was positively correlated with Verrucomicrobia, Coriobacteriia, Thermoleophilia, Ignavibacteria, Coriobacteriales, Nitrosomonadales, Coriobacteriaceae, and Blautia, but was negatively correlated with Betaproteobacteria, Burkholderiales, and Alcaligenaceae. Conclusion. Our findings show compositional alterations of intestinal microbiota in adult patients with INS and correlations between significantly altered taxa and clinical parameters, which points out the direction for the development of new diagnostics and therapeutic approaches targeted intestinal microbiota.

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Section: Discussionmentioning

confidence: 99%

Gut Microbiota Profile in Adult Patients with Idiopathic Nephrotic Syndrome

Lin

You

et al. 2021

BioMed Research International

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“…R. ilealis , which is a Gram-positive obligately anaerobic bacterium and the dominant member of the ileal microbiota, is a strain that can utilize carbohydrates via different and partially redundant pathways and has not been proven harmful so far ( Gerritsen et al, 2017 , 2018 ). Limited research has demonstrated that R. ilealis is at a higher proportion in the gut microbiota of patients with neurodevelopmental disorders, type 2 diabetes, or dementia ( Araos et al, 2018 ; Kang et al, 2019 ; Frazier and Leone, 2020 ). However, some researchers have classified R. ilealis as a probiotic listed with Lactobacillus , which implies that the elevation of Lactobacillus and Romboutsia may represent a kind of beneficial alteration of the gut ( Wu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Preventive Effects of Bacillus licheniformis on Heat Stroke in Rats by Sustaining Intestinal Barrier Function and Modulating Gut Microbiota

Wang²,

Chen³

et al. 2021

Front. Microbiol.

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Heat stroke (HS) models in rats are associated with severe intestinal injury, which is often considered as the key event at the onset of HS. Probiotics can regulate the gut microbiota by inhibiting the colonization of harmful bacteria and promoting the proliferation of beneficial bacteria. Here, we investigated the preventive effects of a probiotic Bacillus licheniformis strain (BL, CMCC 63516) on HS rats as well as its effects on intestinal barrier function and gut microbiota. All rats were randomly divided into four groups: control (Con) + PBS (pre-administration with 1 ml PBS twice a day for 7 days, without HS induction), Con + BL group (pre-administration with 1 ml 1 × 108 CFU/ml BL twice a day for 7 days, without HS induction), HS + PBS (PBS, with HS induction), and HS + BL (BL, with HS induction). Before the study, the BL strain was identified by genomic DNA analysis. Experimental HS was induced by placing rats in a hot and humid chamber for 60 min until meeting the diagnostic criterion of HS onset. Body weight, core body temperature, survival rate, biochemical markers, inflammatory cytokines, and histopathology were investigated to evaluate the preventive effects of BL on HS. D-Lactate, I-FABP, endotoxin, and tight-junction proteins were investigated, and the fluorescein isothiocyanate-dextran (FD-4) test administered, to assess the degree of intestinal injury and integrity. Gut microbiota of rats in each group were analyzed by 16S rRNA sequencing. The results showed that pre-administration with BL significantly attenuated hyperthermia, reduced HS-induced death, alleviated multiple-organ injury, and decreased the levels of serum inflammatory cytokines. Furthermore, BL sustained the intestinal barrier integrity of HS rats by alleviating intestinal injury and improving tight junctions. We also found that BL significantly increased the ratios of two probiotic bacteria, Lactobacillus and Lactococcus. In addition, Romboutsia, a candidate biomarker for HS diagnosis, was unexpectedly detected. In summary, BL pre-administration for 7 days has preventative effects on HS that may be mediated by sustaining intestinal barrier function and modulating gut microbiota.

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“…Secondly, a large number of studies show that many bacteria are involved in the production of short-chain fatty acids (SCFAs), such as Faecalibacterium , Ruminiclostridium , Muribaculum , Anaerotruncus , Romboutsia , Turicibacter , and Clostridiales [ [34] , [35] , [36] , [37] , [38] ]. They are beneficial bacteria, so they can ferment and produce SCFA in the intestine.…”

Section: Discussionmentioning

confidence: 99%

Effects of phycocyanin on pulmonary and gut microbiota in a radiation-induced pulmonary fibrosis model

Li¹,

Lu²,

Liu³

et al. 2020

Biomedicine & Pharmacotherapy

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Objective Radiation pneumonia and fibrosis are major clinical complications of radiotherapy for thoracic tumor patients, and may significantly reduce survival and quality of life. At present, no safe and effective radiation protection measures have been approved for clinical use. Phycocyanin, a protein responsible for photosynthesis from Spirulina , has been shown to have a variety of biological activities and to be beneficial for a variety of diseases, including pulmonary fibrosis. However, the preventive and protective effects of phycocyanin on radiation-induced pulmonary fibrosis have not been studied. Design X-ray single dose irradiation was used on the chest of mice to prepare a mouse model of pulmonary fibrosis, from which the effect of phycocyanin on pulmonary histopathologic change, pulmonary fibrosis, the microbiota in lung and gut, LPS, TNF-α, and IL-6 at different time after irradiation were evaluated. Results Phycocyanin alleviated the radiation-induced lung injury and reduced the level of inflammatory factors. Thorax irradiation led to the disorder in microbiota of the lung and gut. The variation trend of the diversity of the two tissues was opposite, but that of the microbiota composition was similar. The phycocyanin intervention regulated the composition of the lung and gut microbiota, transformed them into normal state, and reduced the level of LPS, which significantly reduced the abundance of inflammation-related bacteria, and increased the abundance of probiotics that produce short-chain fatty acids. Conclusion Phycocyanin could regulate the radiation-induced disorder in lung and gut microbiota of mice, and reduce the radiation-induced lung inflammation and fibrosis.

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Compositional alterations of gut microbiota in children with primary nephrotic syndrome after initial therapy

Cited by 32 publications

References 37 publications

Gut Microbiota Profile in Adult Patients with Idiopathic Nephrotic Syndrome

Gut Microbiota Profile in Adult Patients with Idiopathic Nephrotic Syndrome

Preventive Effects of Bacillus licheniformis on Heat Stroke in Rats by Sustaining Intestinal Barrier Function and Modulating Gut Microbiota

Effects of phycocyanin on pulmonary and gut microbiota in a radiation-induced pulmonary fibrosis model

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