Composition, standardization and chemical profiling of Banisteriopsis caapi, a plant for the treatment of neurodegenerative disorders relevant to Parkinson's disease

Abstract

Ethnopharmacological relevance-Banisteriopsis caapi, a woody vine from the Amazonian basin, is popularly known as an ingredient of a sacred drink ayahuasca, widely used throughout the Amazon as a medicinal tea for healing and spiritual exploration. The usefulness of B. caapi has been established for alleviating symptoms of neurological disorders including Parkinson's disease.Aim of the study-Primary objective of this study was to develop the process for preparing standardized extracts of B. caapi to achieve high potency for inhibition of human monoamine oxidases (MAO) and antioxidant properties. The aqueous extracts prepared from different parts of the plant collected from different geographical locations and seasons were analyzed by HPLC for principal bioactive markers. The extracts were simultaneously tested in vitro for inhibition of human MAOs and antioxidant activity for analysis of correlation between phytochemical composition of the extracts and bioactivities.Materials and methods-Reversed-phase HPLC with photodiode array detection was employed to profile the alkaloidal and non-alkaloidal components of the aqueous extract of B. caapi. The B. caapi extracts and standardized compositions were tested in vitro for inhibition of recombinant preparations of human MAO-A and MAO-B. In vitro cell-based assays were employed for evaluation of antioxidant property and mammalian cell cytotoxicity of these preparations. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Results-Among NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript 7-9) were present in high concentrations in dried bark of large branch. A library of HPLC chromatograms has also been generated as a tool for fingerprinting and authentication of the studied B. caapi species. The correlation between potency of MAO inhibition and antioxidant activity with the content of the main active constituents of the aqueous B. caapi extracts and standardized compositions was established. Phytochemical analysis of regular/ commercial B. caapi dried stems, obtained from different sources, showed a similar qualitative HPLC profile, but relatively low content of dominant markers 1, 2, 7, and 9, which led to decreased MAO inhibitory and antioxidant potency compared to B. caapi Da Vine. Conclusion-The ethnopharmacological use of bark of matured stem/large branch of B. caapi as well as whole matured stem is supported by the results obtained in this investigation. Among various constituents of B. caapi, harmine (7), harmaline (6) and tetrahydroharmine (5) are responsible for MAO-A inhibiti...

Efecto de la ingestión de Banisteriopsis caapi y Psychotria viridis ‘Binomio ayahuasca’ en el hipocampo del cerebro de ratas

“…Estos resultados se correlacionan con las obtenidas en nuestro estudio, en la alteración de las neuronas cerebrales. En otros estudios se ha observado la propiedad antidepresiva del binomio ayahuasca en ratas Wistar (12) . Así mismo, la ayahuasca viene siendo investigada en el tratamiento de los desórdenes neurodegenerativos de la enfermedad de Parkinson (13)(14)(15) .…”

In vitro antiviral activity of Brazilian Cerrado plant extracts against animal and human herpesviruses

“…Banisteriopsis caapi is the most studied species of this genus because it is used by some religious groups in Brazil as an ingredient of the drink locally known as ayahuasca. Chemical compounds identified in this plant species include alkaloids, harmine and harmaline (Wang et al, 2010;Santos et al, 2017). B. intermedia belonging to the Malpighiaceae family popularly known as "murici" has been used in folk medicine to treat fever, as diuretic, for skin infections and ulcers.…”

Alcaloides e o chá de ayahuasca: uma correlação dos "estados alterados da consciência" induzido por alucinógenos

“…A molécula de harmina, por exemplo, apresenta significativa atividade anti-tripanossomal contra parasitas do tipo Trypanosoma lewissi e T. cruzii (Sangirard, 1989;Mckenna et al, 1998). Estudos recentes apontam para um possível tratamento contra o mal de Parkinson pelo uso do chá de ayahuasca (Schwarz et al, 2003;Samoylenko et al, 2010;Wang et al, 2010).…”

“…Algumas dessas substâncias são os principais componentes do chá de ayahuasca. et al, 2009;Trich et al, 2009;Samoylenko et al, 2010;Wang et al, 2010;Warren, 2010).…”