Composition, richness and nonrandom assembly of culturable bacterial-microfungal communities in floral nectar of Mediterranean plants

Álvarez‐Pérez, Sergio; Herrera, Carlos M.

doi:10.1111/1574-6941.12027

Cited by 101 publications

(109 citation statements)

References 72 publications

(131 reference statements)

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“…[54], [55]). In particular, we note that Álvarez-Pérez & Herrera [30] found bacteria in 2.6% of the nectar samples of A. aestivus , while we isolated bacteria from all the examined samples. We attribute this to the different culturing methods, since those authors [30], used the rich medium trypticase soy agar (TSA) without sucrose, while we employed R2A agar (which contains far fewer proteins) supplemented with 30% sucrose which better mimics the nectars' nutritional environment.…”

Section: Discussionmentioning

confidence: 47%

“…Still, it may be that not all the Acinetobacter OTUs were identified by culture [28]. We identified 161 representative isolates, which is a relatively large number compared with the number in other studies on culture-dependent bacterial communities in nectar [28], [30], [35] and on nectar-dwelling yeasts (e.g. [54], [55]).…”

Section: Discussionmentioning

confidence: 91%

“…the identification is accurate only up to the genera level [50]. Nevertheless, culturing is a common way to study bacterial communities in some environments such as food fermentation [51], [52] and nectar [29]–[30], [35], [53], where the fraction of culturable bacteria is higher than in other ecological habitats (e.g. marine environments) [28], [51], [52].…”

Section: Discussionmentioning

confidence: 99%

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The Role of Abiotic Environmental Conditions and Herbivory in Shaping Bacterial Community Composition in Floral Nectar

et al. 2014

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Identifying the processes that drive community assembly has long been a central theme in ecology. For microorganisms, a traditional prevailing hypothesis states that “everything is everywhere, but the environment selects”. Although the bacterial community in floral nectar may be affected by both atmosphere (air-borne bacteria) and animals as dispersal vectors, the environmental and geographic factors that shape microbial communities in floral nectar are unknown. We studied culturable bacterial communities in Asphodelus aestivus floral nectar and in its typical herbivorous bug Capsodes infuscatus, along an aridity gradient. Bacteria were sampled from floral nectar and bugs at four sites, spanning a geographical range of 200 km from Mediterranean to semi-arid conditions, under open and bagged flower treatments. In agreement with the niche assembly hypothesis, the differences in bacterial community compositions were explained by differences in abiotic environmental conditions. These results suggest that microbial model systems are useful for addressing macro-ecological questions. In addition, similar bacterial communities were found in the nectar and on the surface of the bugs that were documented visiting the flowers. These similarities imply that floral nectar bacteria dispersal is shaped not only by air borne bacteria and nectar consumers as previously reported, but also by visiting vectors like the mirid bugs.

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Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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The Role of Abiotic Environmental Conditions and Herbivory in Shaping Bacterial Community Composition in Floral Nectar

et al. 2014

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“…Firstly, bacteria were ๙ever detected i๙ our microscopical (×400 a๙d ×1000 mag๙ificatio๙) observatio๙s of both i๙oculated a๙d co๙trol samples. A๙d seco๙dly, prior culture-based assessme๙t of yeast a๙d bacteria abu๙-da๙ce i๙ H. foetidus a๙d R. officinalis has show๙ that, eve๙ whe๙ prese๙t, bacteria are ge๙erally a๙ u๙importa๙t compo๙e๙t of the microbial commu๙ities i๙ the ๙ectar of these species (Álvarez-Pérez & Herrera, 2013). Furthermore, the prese๙ce of yeasts other tha๙ M. reukaufii a๙d M. gruessii i๙ i๙oculated samples was too occasio๙al to have a๙y importa๙t effect o๙ our results.…”

Section: Discussionmentioning

confidence: 63%

Comparative effects of two species of floricolous <em>Metschnikowia</em> yeasts on nectar

Canto¹,

Herrera²,

García³

et al. 2015

Anal. Jard. Bot. Madr.

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Canto, A., Herrera, C.M., García, I.M., García, M., Bazaga, P. 2015.Comparative effects of two species of floricolous Metschnikowia yeasts on nectar. Anales Jard. Bot. Madrid 72(1): e019Nectar yeast communities in southern Spain are dominated by two closely-related species, Metschnikowia reukaufii Pitt & M.W. Mill. and M. gruessii Gim.-Jurado (Ascomycota, Saccharomycetales), although they tend to be distributed differentially across different host plants. We explore here the possibility that the two yeasts play different functional roles in floral nectar by differing in their impact on sugar concentration and composition of nectar. Experiments were undertaken under controlled conditions using bumblebees caught foraging on the flowers of two different host plants each of which is known to harbor predominantly one of the two yeasts. Bumblebees were used as sources of inocula to obtain two groups of samples from the nectar of Helleborus foetidus L. (Ranunculaceae): nectar samples inoculated with M. gruessii and samples inoculated with M. reukaufii. Metschnikowia gruessii was poorly represented in nectar samples, while M. reukaufii was by far the most common and had the highest cell density. Although the two yeasts caused relatively similar changes in nectar sugar composition, which involved increasing fructose and decreasing sucrose proportions, they marginally differed in their quantitative impact on total nectar sugar concentration. Results suggest that differential yeast occurrence across host plants may lead to yeast specialization and modify the outcomes of the plant-pollinator interface. . Ambas levaduras son especies muy cercanas entre sí, pero se distribuyen diferencialmente entre las especies de plantas cuyo néctar las hospeda. En este trabajo se explora la posibilidad de que las levaduras tengan funciones distintas impactando diferencialmente la concentración y composición del néctar. Para esto se realizaron experimentos bajo condiciones controladas usando individuos de abejorros que fueron capturados cuando visitaban flores que albergan predominantemente una de las dos posibles levaduras mencionadas. Los abejorros fueron utilizados como agentes inoculantes naturales en el néctar de Helleborus foetidus L. para obtener dos grupos de muestras, unas con M. reukaufii y otras con M. gruessii. Los resultados mostraron que M. gruessii estuvo pobremente representada en las muestras; en contraste, M. reukaufii fue la más frecuente y de mayor densidad en el néctar. Aun cuando ambas levaduras tuvieron un efecto similar en la composición proporcional de los azúcares en el néctar, aumento de la fructosa y decremento de la sacarosa, las dos especies difieren marginalmente en su impacto en la concentración total de azúcares. Los resultados sugieren que la ocurrencia diferencial de las especies de levaduras entre sus plantas hospederas, puede conducir a una modificación en las interacciones entre las plantas y sus visitantes florales y también a una especialización de levaduras por hospedero.

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“…Studies on microbial diversity conducted by Brysch-Herzberg (2004), Pozo, Herrera & Bazaga (2011), Álvarez Pérez & Herrera (2013), Jacquemyn et al (2013) and Mittelbach et al (2015) revealed that floral nectar is frequently colonized by specialized sugar-consuming yeasts in the Ascomycota and Basidiomycota phyla, along with several bacterial groups. However, most studies of nectar-living microorganisms have been conducted in temperate areas; knowledge of nectar microbial diversity in tropical habitats remains poor.…”

Section: Introductionmentioning

confidence: 99%

Nectar-living yeasts of a tropical host plant community: diversity and effects on community-wide floral nectar traits

Canto

Herrera

Rodríguez

2017

PeerJ

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We characterize the diversity of nectar-living yeasts of a tropical host plant community at different hierarchical sampling levels, measure the associations between yeasts and nectariferous plants, and measure the effect of yeasts on nectar traits. Using a series of hierarchically nested sampling units, we extracted nectar from an assemblage of host plants that were representative of the diversity of life forms, flower shapes, and pollinator types in the tropical area of Yucatan, Mexico. Yeasts were isolated from single nectar samples; their DNA was identified, the yeast cell density was estimated, and the sugar composition and concentration of nectar were quantified using HPLC. In contrast to previous studies from temperate regions, the diversity of nectar-living yeasts in the plant community was characterized by a relatively high number of equally common species with low dominance. Analyses predict highly diverse nectar yeast communities in a relatively narrow range of tropical vegetation, suggesting that the diversity of yeasts will increase as the number of sampling units increases at the level of the species, genera, and botanical families of the hosts. Significant associations between specific yeast species and host plants were also detected; the interaction between yeasts and host plants impacted the effect of yeast cell density on nectar sugars. This study provides an overall picture of the diversity of nectar-living yeasts in tropical host plants and suggests that the key factor that affects the community-wide patterns of nectar traits is not nectar chemistry, but rather the type of yeasts interacting with host plants.

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Composition, richness and nonrandom assembly of culturable bacterial-microfungal communities in floral nectar of Mediterranean plants

Cited by 101 publications

References 72 publications

The Role of Abiotic Environmental Conditions and Herbivory in Shaping Bacterial Community Composition in Floral Nectar

The Role of Abiotic Environmental Conditions and Herbivory in Shaping Bacterial Community Composition in Floral Nectar

Comparative effects of two species of floricolous <em>Metschnikowia</em> yeasts on nectar

Nectar-living yeasts of a tropical host plant community: diversity and effects on community-wide floral nectar traits

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