Composition of the Vitreous Humor in Relation to That of Plasma and Aqueous Humors

Reddy, Divya; Kinsey, V. Everett

doi:10.1001/archopht.1960.00950020717015

Cited by 87 publications

(37 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The levels of ascorbic and lactic found in the normal rabbit vitreous agree with those of previous investigators [1][2][3][4][5][6]. Only the protein concentration value does not correspond with those of previous studies [1,2,5], although there is considerable variation among the lev els reported by other authors, ranging from 0.1 [2] to 0.9 rng/ml [5].…”

Section: Control Groupsupporting

confidence: 75%

“…Only the protein concentration value does not correspond with those of previous studies [1,2,5], although there is considerable variation among the lev els reported by other authors, ranging from 0.1 [2] to 0.9 rng/ml [5].…”

Section: Control Groupcontrasting

confidence: 73%

“…Lactic acid values in the aqueous and vit reous humors are higher than the plasma val ues [ 1,2,4], The intraocular sources of lactate are the tissues with significant glycolytic ac tivity, such as the retina, the ciliary body, the lens and the cornea [4. 24],…”

Section: Experimental Groups Variations O F Total Protein Concentratimentioning

confidence: 99%

“…Much of them tabulated the basic chemistry of aqueous and vitreous humors on several species [1,2], More detailed studies on the composition of these fluids, including sepa rate analysis of reducing substances (e.g. sug- Results are means ± SD * p < 0.01.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Manzanas¹,

Pastor²,

Muñoz

et al. 1992

Ophthalmic Res

View full text Add to dashboard Cite

Vitrectomy and the subsequent vitreous fluid exchange alter the chemical and physical properties of the vitreous. This study assessed the time course alterations of total protein, ascorbic acid and lactic acid in the rabbit vitreous cavity following vitrectomy. Normal values were 2.2 ± 1.2 mg/ml, 8.5 ± 1.0 mg/l00 ml and 16.8 ± 3.5 μmol/ml, respectively. Nine groups of 5–10 adult albino rabbits underwent closed vitrectomy using physiologic saline, Ringer’s lactate and BSS plus solutions. Samples were withdrawn at 3 and 24 h and 7 days after vitrectomy. Total protein levels increased in all groups at 3 and 24 h (20-30 mg/ml). Ascorbic acid measurements at 24 h rose only with saline and BSS plus. At 24 h the BSS plus group was the only one demonstrating an increased lactic acid concentration.

show abstract

Section: Control Groupsupporting

confidence: 75%

Section: Control Groupcontrasting

confidence: 73%

Section: Experimental Groups Variations O F Total Protein Concentratimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Manzanas¹,

Pastor²,

Muñoz

et al. 1992

Ophthalmic Res

View full text Add to dashboard Cite

show abstract

“…Ascorbic acid plays a cytotoxic role for vascular endothelial cells, the latter being relatively susceptible to ascorbyl radicals, thereby functioning as an inhibitor of neovascularization (Hanashima and Namiki, 1999). The concentration of ascorbic acid in the vitreous has been reported in a number of species, including rabbits (0.07 mg/ml; Reddy and Kinsey, 1960;McGahan, 1985), guinea pigs (0.15 mg/ml; Berger et al, 1988), and humans (0.12 mg/ml; Takano et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Vitreous humor and albumin augment the proliferation of cultured retinal precursor cells

Yang

Klassen

Pries

et al. 2008

J of Neuroscience Research

View full text Add to dashboard Cite

Intravitreal injection is an important delivery route for studies involving the transplantation of various types of precursor cells to the retina; however, the effect on these cells of exposure to the vitreous microenvironment has not been specifically investigated. Here vitreous humor was evaluated for the potential to influence the proliferation of rat retinal precursor cells in vitro. Cells were isolated at embryonic day 19 and plated in standard proliferation medium in the presence or absence of fluid expressed from porcine vitreous humor. Cellular proliferation at different concentrations of vitreous fluid supplementation was quantified by using a (3)H-thymidine incorporation assay. Active components of vitreous fluid were partially characterized by gel filtration chromatography (GFC) and UV spectral analysis. The effect of each vitreous fraction on proliferation was determined as well. Results showed that addition of 20% vitreous fluid to primary rat retinal cultures significantly increased (3)H-thymidine incorporation compared with growth medium without vitreous supplementation. A vitreous fraction showing growth-promoting activity was localized to a molecular mass range <1000 Da, consistent with ascorbic acid. Ascorbic acid was confirmed in vitreous fluid by UV spectral analysis. Growth-augmenting activity was present in higher molecular mass vitreous fractions, consistent with protein components. Albumin, the major protein in vitreous fluid, was found to augment proliferation. Because vitreous-associated augmentation of retinal precursor proliferation remains an epidermal growth factor-dependent phenomenon, the proliferative status of transplanted cells in the vitreous cavity is likely determined by a combination of factors.

show abstract

The Lens and Vitreous

Ar¹

1960

Archives of Ophthalmology

View full text Add to dashboard Cite

Composition of the Vitreous Humor in Relation to That of Plasma and Aqueous Humors

Cited by 87 publications

References 20 publications

Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Vitreous humor and albumin augment the proliferation of cultured retinal precursor cells

The Lens and Vitreous

Contact Info

Product

Resources

About