Composition of the Regenerating Liver After Partial Hepatectomy in Normal and Protein-Depleted Rats

Gurd, Fraser N.; Vars, Harry M.; Ravdin, I. S.

doi:10.1152/ajplegacy.1947.152.1.11

Cited by 32 publications

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“…The individual components of liver appear generally to return to normal concentration between the 7th and 14th postoperative days approximately. Gurd et al (1948) found practically no difference in total protein content of normal and 14-day regenerating liver; the concentration of collagen in liver is so low that such changes in its concentration as occur do not affect the total protein content appreciably. Total lipid (Bogetti & Mazzocco, 1939;Gurd et al, 1948), neutral fat, total phospholipid, free and ester cholesterol concentrations (Ludewig, Minor & Hortenstine, 1939), are practically normal by the end of the first week after partial hepatectomy.…”

Section: Discussionmentioning

confidence: 73%

“…Gurd et al (1948) found practically no difference in total protein content of normal and 14-day regenerating liver; the concentration of collagen in liver is so low that such changes in its concentration as occur do not affect the total protein content appreciably. Total lipid (Bogetti & Mazzocco, 1939;Gurd et al, 1948), neutral fat, total phospholipid, free and ester cholesterol concentrations (Ludewig, Minor & Hortenstine, 1939), are practically normal by the end of the first week after partial hepatectomy. Glycogen is back to normal concentrations by the end of the first postoperative week according to Gurd et al (1948), though Stone (1935) Drabkin (1947).…”

Section: Discussionmentioning

confidence: 73%

“…Total lipid (Bogetti & Mazzocco, 1939;Gurd et al, 1948), neutral fat, total phospholipid, free and ester cholesterol concentrations (Ludewig, Minor & Hortenstine, 1939), are practically normal by the end of the first week after partial hepatectomy. Glycogen is back to normal concentrations by the end of the first postoperative week according to Gurd et al (1948), though Stone (1935) Drabkin (1947). This raised DNA concentration may represent an increase in the population of cells of the macrophage type, reported by Abercrombie & Harkness (1951) as present on the 7th but not on the 21st day.…”

Section: Discussionmentioning

confidence: 99%

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Further observations on collagen in regenerating liver of the rat

Harkness¹,

Harkness²

1954

The Journal of Physiology

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In a previous paper, experiments were described in which the collagen content of the rat's liver, regenerating after partial hepatectomy, was used as an indication of the growth of supporting tissue (Harkness, 1952 a). Regeneration of collagen was found to lag far behind that of the liver as a whole, and was apparently incomplete 3 weeks after partial hepatectomy when the liver parenchyma is fully regrown. Collagen, however, was estimated chemically as a whole, and, consequently, the morphological significance of the changes was not clear. True collagen, judged by staining properties, is present in the peritoneal capsule of the liver, and in the walls and tissues surrounding the blood vessels and bile ducts. In addition, there is a network of reticulin in walls of the hepatic sinusoids. The available evidence indicates that this material is similar to collagen chemically (Bowes & Kenten, 1949), and the term collagen will be used to include it.We thought that the regeneration of collagen at 3 weeks, although it did not reach the original level, might nevertheless be complete in the sense that it had reached an equilibrium state representing a somewhat altered liver structure. There are features of 3-week regenerated liver which lead one to suspect that it might be constructed with less than the normal proportion of collagen. Thus the lobes become more rounded with less relative surface area, so one might expect the capsular collagen to increase less than the weight of the liver. The fact that the lobes are more rounded should also make it unnecessary for the main vascular tree to expand as much proportionately as the weight. On the other hand, one might expect the sinusoids to reach their normal size relative to the parenchymal cells and hence to increase approximately in proportion to the weight of the liver. In order to clarify our previous results we have therefore separated the collagen in the liver into three fractions-capsular, vascular and parenchymal, the latter representing the collagen of the vessels most closely associated with the parenchyma-and estimated the collagen in these separate fractions at 3 and 6 weeks after partial hepatectomy. At 6 weeks the

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

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Further observations on collagen in regenerating liver of the rat

Harkness¹,

Harkness²

1954

The Journal of Physiology

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“…Regarding the net increase in protein, the only direct estimations appear to be those of Gurd, Vars & Ravdin (1948), Szego & Roberts (1949), and Roberts (1953). There are also estimations of total nitrogen (Brues et at.…”

Section: Plasma Amino-acidsmentioning

confidence: 99%

Free amino‐acids in liver and blood after partial hepatectomy in normal and adrenalectomized rats

Ferrari¹,

Harkness²

1954

The Journal of Physiology

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It is well known that, after removal of part of the liver of the rat, the remainder grows rapidly to reach the normal size in 2-3 weeks (Higgins & Anderson, 1931). Though many aspects of this growth process have been investigated, little attention has been paid to changes in amino-acid metabolism. Since the liver plays a prominent part in the metabolism of these substances, one would expect that there might be changes in their concentration, both in the liver and in the blood, after partial hepatectomy, and that these changes might even be related causally to the subsequent regeneration. Christensen, Rothwell, Sears & Streicher (1948) have reported a considerable rise in the concentration of free amino-acids of rat liver cells 1-3 days after partial hepatectomy, though they found no significant change in the blood plasma; Des Marais & Dugal (1948), on the other hand, found a rise in free amino-acids of plasma in the first day after partial hepatectomy. Christensen et al. (1948) give some data on glycine and glutamine, and the peptide glutathione, but this appears to be the only information available at present. Thus although there is some information about a change in total concentration of amino-acids in liver and blood, of the individual compounds little is known. The work described here was undertaken with the object of filling this gap. For this purpose the technique of filter-paper chromatography was chosen. It was felt that, in a preliminary investigation of this sort, the disadvantages of this method on the quantitative side were outweighed by its simplicity and wide application, and that it would be more useful to have information about a wide range of substances within fairly wide limits of accuracy, than more exact information about a few. This method has been used in conjunction with estimation of total free carboxyl-amino nitrogent (Hamilton & Van Slyke, 1943) in liver and plasma.The investigation was confined to the first 24 hr after partial hepatectomy. t This term will be shortened to amino-nitrogen in the rest of this paper.

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“…It appears that these two processes, the increase in liver protein and the increase in plasma protein production, behave as separate systems and follow different time courses. Figures of total liver protein, total N and fatfree-glycogen-free solid of liver (Gurd, Vars & Ravdin, 1948;Szego & Roberts, 1949;Roberts, 1953;Brues, Drury & Brues, 1936;Harkness, 1952a) indicate that the maximum rate of increase in liver protein per unit present occurs in the second half of the first postoperative day (12-24 hr) with a subsequent decline. Changes in plasma protein production may be assessed from change in the plasma protein concentration after partial hepatectomy (Chanutin, Hortenstine, Cole & Ludewig, 1938), if certain assumptions are made.…”

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confidence: 99%