Composition of the milt of some teleost fishes

Abstract

The milt composition of six freshwater teleosts was studied, and the measured parameters showed clear specific differences between species. The highest spermatocrits and sperm densities were observed in perch, Percafluviatilis, and burbot, Lota Iota, and the lowest in rainbow trout, Salmo gairdneri, and whitefish, Coregonus lavaretus. Fructose concentrations in the seminal plasma were small compared to mammalian values. The glucose concentrations in the seminal plasma were five times higher than those of fructose, and higher in landlocked salmon and rainbow trout than in the other species. The citric acid concentration of all species except whitefish showed a significantly positive correlation to either spermatwrit or sperm density. The role of citric acid in the seminal plasma of fishes was also assumed to be important. The glycerol concentration in the seminal plasma was comparatively high, and highest in whitefish. This was assumed to be related to the high applicability value of glycerol as a cryoprotective agent for whitefish sperm. The high glycerol concentration was also assumed to be related to the lipolytic capacity of the testis in the studied species.

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

“…The total protein concentration in the present study (0.1-0.9, 0.4 ± 0.2 mg/ml) was lower than that reported for salmonids such as rainbow trout: 1.74 ± 0.79 mg/ml (Loir et al, 1990), 1.34 ± 0.67 mg per ml (Ciereszko and Dabrowski, 1993) and 1.47 ± 0.84 mg/ml (Lahnsteiner et al, 1998). In addition, the protein content is significantly different from that of non-salmonid fish (Piironen and Hyvarinen, 1983;Lahnsteiner et al, 1995;Lahnsteiner et al, 1996). Mostly, the seminal plasma of teleost fish is characterized by a very low concentration of proteins in comparison with mammalian semen and does not exceed 2 g/l (Krol et al, 2006).…”

“…Different values of sperm production were also recorded for each group during the spawning season. Several studies have mentioned that the differences in sperm production could be related to many factors including the age and weight of the male (Suquet et al, 1994(Suquet et al, , 1998, ecology and spawning behaviour of broodstock (Piironen and Hyvarinen, 1983) and sampling period and method (Suquet et al, 1994). In present study, there was no significant relationship between broodstock weight and sperm production.…”

“…(1) Composition of seminal plasma: seminal plasma has a unique composition regarding the presence of organic and inorganic components which support the viability of spermatozoa as well as substances reflecting the function of the reproductive system like spermatozoa (Piironen and Hyvarinen, 1983;Lahnsteiner et al, 1993 Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season Ciereszko et al, 2000). In this regard, the inorganic components present in seminal plasma (potassium, sodium, magnesium, calcium, chloride, pH and osmolality) provide isotonic conditions for spermatozoa and inhibit sperm motility (Morisawa et al, 1983).…”

Sperm characteristics in perch Perca fluviatilis L.

“…Piironen & Hyvarinen (1983) found similar sperm densities in perch, ranging from 37 Â 10 9 to 127 Â 10 9 sperm ml ÿ1 , but without specifying the M of the examined fish. As body mass to milt volume ratio is subject to annual variation (P. Compared to other fish species (Piironen & Hyvarinen, 1983;Stockley et al, 1996;Mylonas et al, 2003;Rainis et al, 2003;Mansour et al, 2004), the average sperm density in perch is high and the variation between males is considerably large. Sperm density in externally fertilizing fish has been shown to be generally higher than in mammals or other internal fertilizers (Stockley et al, 1997;Petersen & Warner, 1998;Byrne et al, 2002).…”

Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility

“…Seminal plasma composition in salmonids has been carefully studied particularly in rainbow trout and salmon (Piironen and Hyvarinen, 1983;Munikittrick and Moccia, 1987;Glogowski et al, 2000). The composition of seminal plasma in other salmonid species has been examined scarcely so far (Cruea, 1969;Morisawa et al, 1979;Piironen and Hyvarinen, 1983;Hatef et al, 2007).…”