Composition of the lipid droplet in embryos of the annual fish Nothobranchius guentheri

Brind, Joel; Al-Ani, Enas; Matias, Jonathan R.; Markofsky, Jules; Rizer, Ronald L.

doi:10.1016/0305-0491(82)90336-4

Cited by 9 publications

(13 citation statements)

References 13 publications

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“…phospholipids associated with the lipovitellins, and the non-polar lipids, i.e. triacyglycerols, wax esters or cholesteryl esters, of the oil globules (Nakagawa and Tsuchiya 1971;Léger et al 1981;Brind et al 1982;Eldridge et al 1983;Moodie et al 1989). These lipids are rich in polyunsaturated fatty acids of the (n-3) series, crucial for embryonic and larval development (Henderson and Tocher 1987;Silversand et al 1996;Wiegand 1996).…”

Section: Cloning Of the Putative Ldl Receptor-binding Domain Of Turbomentioning

confidence: 99%

Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development

Poupard

André

Durliat

et al. 2000

Cell and Tissue Research

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During embryogenesis of teleost fish, the formation of a yolk syncytial layer (YSL) enables the resorption of the yolk reserves and development up to the larval stage. We have examined the changes of the yolk cell structure in relation to yolk and oil-globule lipid utilization during development of the turbot (Scophthalmus maximus). After encapsulation by the YSL, resorption of the single, large oil globule occurred predominantly after yolk resorption and was slower in fasting larvae. The YSL was in contact with an enlarged perisyncytial space, but no vascular network or red blood cells were present within the walls of the yolk sac. Intrasyncytial channels infiltrated by pigmented lining cells were observed in the YSL surrounding the oil globule. Apolipoprotein E (apoE) has a prominent role in lipid metabolism because of its ability to interact with lipoprotein receptors. We performed molecular cloning of the putative low-density lipoprotein-receptor binding domain of turbot apoE. In situ hybridization analysis revealed a very high level of apoE transcripts in the YSL, while no expression could be detected in the intestine. YSL apoE expression was correlated with the synthesis of very low density lipoprotein (VLDL) particles. An extraordinarily high number of VLDL particles were poured into the perisyncytial space, and intrasyncytial channels enabled the transfer of yolk- and oil globule-derived lipids to the developing embryo or larva. The pattern of apoE mRNA distribution in relation to YSL lipoprotein synthesis indicates that apoE expression is a suitable molecular marker for monitoring endogenous lipid nutrition during the endoexotrophic period of teleost fish development.

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Section: Cloning Of the Putative Ldl Receptor-binding Domain Of Turbomentioning

confidence: 99%

Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development

Poupard

André

Durliat

et al. 2000

Cell and Tissue Research

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“…Rather, lipid extracts have been prepared from whole ovaries, ovulated eggs or embryos. Because lipoprotein yolk in species possessing discrete oil droplets also contains neutral lipid (L~ger et al, 1981;Brind et al, 1982;Eldridge et al, 1983;Ando and Hatano, 1991), this approach reduces the precision of knowledge concerning the compartmentalization of the various neutral lipid classes in the different yolk components. Many studies have also been performed using ovaries sampled during recrudescence rather than ovulated eggs.…”

Section: General Considerations and Interpretive Problemsmentioning

confidence: 99%

“…In striped bass, wax and steryl ester made up 90% of the egg oil (Eldridge et al, 1983). TAG was found to be the major neutral lipid in the oils from oocytes of four species of Oncorhynchus (Lrger et al, 1981;Ando and Hatano, 1991) and that of the embryos of an annual East African fish (Nothobranchius guentheri, Aplocheilidae) (Brind et al, 1982). In the walleye, the oil contains both TAG (55%) and steryl and wax esters (Moodie et al, 1989).…”

Section: Isolated Oil and Lipoprotein Yolkmentioning

confidence: 99%

“…In several species, part or even all of the droplet may be retained as a caloric reserve in the event of restricted availability of appropriate food during the early stages of feeding. With adequate food availability, the oil may then be consumed rapidly to enhance growth rates (Eldridge et al, 1981(Eldridge et al, , 1982Brind et al, 1982;Kohno et al, 1986;Moodie et al, 1989). Studies like that of Craik and Harvey (1987) would be useful for investigating the contribution of oil to buoyancy in larvae, especially those which rely on a major osmolyte, amino acid, as the primary energy source before the consumption of the oil (Ronnestad et al, 1992(Ronnestad et al, , 1994.…”

Section: Summary and Biological Significancementioning

confidence: 99%

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Composition, accumulation and utilization of yolk lipids in teleost fish

Wiegand

1996

Rev Fish Biol Fisheries

363

277

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“…Embryos lacking oil droplets from an early stage of development developed normally to fry. These results might suggest that the embryo does not require the indispensable materials from the oil droplets for development, although it has been postulated that oil droplets store nutrient substances of high caloric value (Kryzhanovskii 1960;Brind et al 1982) and vitamin A (carotin) (Kunz 1964). On the other hand, if liquid paraffin containing steroid (Hishida 1964) and various oils (Takeuchi 1965) are injected into the ooplasm of developing Oryzias eggs, these oils are absorbed, but do not interfere with development.…”

Section: Discussionmentioning

confidence: 88%

Oil droplets and yolk spheres during development of Medaka embryos

2008

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The sizes of oil droplets (globules) and the yolk sphere in the Medaka Oryzias latipes egg were measured in the developmental period from fertilization to hatching. Oil droplets coalesced with one another in the process of shifting toward the vegetal pole, and a single large oil droplet was finally located at the vegetal pole region in most eggs 2 days post-fertilization. The volume of the yolk sphere steeply decreased in the period from 2 to 8 days post-fertilization. The volume of oil droplets also declined linearly from 4 to 10 days post-fertilization. Lipid components exhibited no distinct change during embryogenesis. In order to verify whether oil droplets were required for development of Medaka embryos, oil droplets were artificially removed from the early developing embryos without the chorion (egg envelope). Naked embryos without the oil droplet developed normally to fry in the sterilized incubation medium and grew to the same mature fry as those grown from the control embryos.

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Composition of the lipid droplet in embryos of the annual fish Nothobranchius guentheri

Cited by 9 publications

References 13 publications

Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development

Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development

Composition, accumulation and utilization of yolk lipids in teleost fish

Oil droplets and yolk spheres during development of Medaka embryos

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