an inexpensive byproduct, especially using a simple, rapid and low cost process. Crude rice bran oil CRBO contains approximately 1.5-2.9 of γ-oryzanol. However, more than 90 of the initial amount of γ-oryzanol is lost and removed with the rice bran oil soapstock RBOS during the alkali refining process 8. Thus, in industrial practice, the RBOS and its acidulated form known as rice bran acid oil RBAO are excellent sources for recovery of γ-oryzanol. Several processes have been proposed for recovery of the γ-oryzanol from CRBO, RBOS and RBAO 8 14. These processes can be divided into two major consecutive pro-Abstract: A rapid and low energy consumption method for the recovery of γ-oryzanol from rice bran acid oil (RBAO), a byproduct of rice bran oil (RBO) refining, is presented. The RBAO was converted to the fatty acid ethyl ester (FAEE) and was used as the starting material. The dissolved γ-oryzanol was separated from the FAEE using an acid-base extraction method with alkaline aqueous ethanol and hexane as extraction media. A systematic investigation of the extraction yield was carried out by applying response surface methodology (RSM) based on central composite design (CCD) and Derringer's desirability function. The concentration of NaOH, the percentage of ethanol in water, the hexane content and their interactions showed significant effects on the yield of γ-oryzanol and FAEE. The optimal extraction conditions were as follows: extraction time of 1 min at room temperature (28-32℃); extraction medium: 1.855 M NaOH; 75.91% ethanol in water and 20.59% hexane in the total volume of the extractant; and FAEE to extractant ratio of 1:10 corresponding to a maximum γ-oryzanol yield of 75.82±3.44% and the desired FAEE yield of 54.42±7.80%. The γ-oryzanol-rich fraction was further purified by washing with a 2% Na 2 CO 3 solution, obtaining 69.94% recovery yield with 89.90% purity of γ-oryzanol. The purified γ-oryzanol showed good scavenging activity on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and the ABTS radical and was comparable to the commercial product, clearly suggesting that the presented process was efficient and feasible.