Composition of Nonanthocyanin Polyphenols in Alcoholic-Fermented Strawberry Products Using LC–MS (QTRAP), High-Resolution MS (UHPLC-Orbitrap-MS), LC-DAD, and Antioxidant Activity

Abstract

In this study, the nonanthocyanin (poly)phenolic profile of an alcoholic-fermented strawberry beverage was characterized. High-performance liquid chromatography coupled with a triple-quadropole mass spectrometer and ultra-high-performance liquid chromatography coupled with a linear trap quadropole and an Orbitrap mass analyzer was used to identify nonanthocyanin phenolic compounds. Sixty-six compounds were identified, and 13 of these were identified for the first time in strawberry or its derived alcoholic fermented beverage: protocatechuic acid-4-O-β-hexoside, brevifolin carboxylic acid, ferulic acid glucuronide, dimer caffeic acid-O-hexoside, luteolin-3'-O-xyloside, isorhamnetin 3-O-glucoside, taxifolin-O-glucoside, (+)-aromadendrin rhamnoside, eriodictyol-7-O-glucoside, (+)-taxifolin, (+)-aromadendrin, eriodictyol, and homovanillic acid. The alcoholic fermentation process produced significant increases in certain compounds, such as homovanillic acid and p-hydroxybenzoic acid, while a significant decrease in galloyl bis-HHDP-glucose was observed. Linear discriminant analysis correctly classified samples initial, final, and pasteurized, which led to the conclusion that alcoholic fermentation induces significant changes in composition, mainly in relation to the 19 compounds represented in the tables of this work.

Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro

“…Recent studies have revealed that catechins exhibit anti-inflammatory activity via wide range of mechanisms [45,46]. Figure S13), as proposed by [48]. The product ion of C33 occurred at m/z 285 [M-H-308] − , and the compound was tentatively identified as kaempferol-O-neohesperidoside (Supplementary Figure S14).…”

An Overview of Plant Phenolics Measurement

“…92-94 TOF and Orbitrap™ technology provide the possibility of accurate mass determination which is a major step toward identification of unknowns especially when coupled with MS/MS fragmentation data. [95][96][97][98][99][100][101][102][103] In addition to UV, PDA, and MS, other detection methods for liquid chromatographic separations have been successfully applied over the years, including chemiluminescence reaction detectors, fluorescence detectors, and electrochemical detectors, especially for quantitation. [104][105][106][107][108][109][110][111] Gas chromatography/mass spectrometry (GC/MS) has been employed for the analysis of phenolic microbial fermentation products in biological samples in order to study the metabolic impact of flavonoid consumption [112][113][114] but has also been used for the direct analysis of the polyphenolic content of plant material.…”

Polyphenolic Profile of Pear Leaves with Different Resistance to Pear Psylla (Cacopsylla pyri)

“…36 Identification of flavonol and flavone glycosides was largely based on the MS/MS data (Table 3). 37 In the analyzed leaf extracts it was possible to identify hexosides (loss of 162 Da), acetyl-hexosides (loss of 204 Da), rutinosides (loss of 308 Da), and one derivative of p-coumaroyl-hexoside. Regarding flavonol glycosides, derivatives of kaempferol, quercetin, and isorhamnetin were found, giving MS/MS base peaks belonging to the deprotonated aglycones at m/z 285, 301, and 315, respectively.…”