Composition of nifH in a wastewater treatment system reliant on N2 fixation

Bowers, Tracey; Reid, Nicola M.; Lloyd‐Jones, Gareth

doi:10.1007/s00253-008-1486-2

Cited by 22 publications

(16 citation statements)

References 27 publications

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“…SIUL was the dominant diazotroph covering 50.5% (46/91) of the clone library. These protein sequences showed high similarity to sequences previously obtained from various environments, including rhizospheres, soil, corals, coast, salt marsh, eastern Mediterranean Sea, and wastewater (Man-Aharonovich et al, 2007;Bowers et al, 2008). The protein sequences L2-H1, L2-H9 and L2-H10 showed high identity with Dechloromonas sp.…”

Section: Nifh Diversity In Station L2supporting

confidence: 60%

Diversity of nifH gene sequences in the sediments of South China Sea

Wu¹

2011

Afr. J. Microbiol. Res.

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In order to contribute to knowledge about structure of marine diazotrophic communities in the sediments of South China Sea, the molecular diversity of the nifH gene, which encodes the Fe protein of the nitrogenase complex, was assessed by polymerase chain reaction (PCR) amplification using PolF/R primers, followed by cloning and sequencing. Sequences of nifH genes were amplified from environmental deoxyribonucleic acid (DNA) samples collected during three stations including shallow sea (75 m, station L10), shelf (450 m, station L2) and deep sea (1000 m, station L21), and covering an area between 17 to 19°N and 111 to 119°E. Samples from shallow sea contained β-, and δproteobacteria; the shelf contained α-, β-, δ-proteobacteria; the deep sea contained α-, δ-, γproteobacteria, firmicutes, and green nonsulfur (GNS) bacterium. These results suggested that diazotroph was significant component potentially contributing to nitrogen fixation in South China Sea.

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Section: Nifh Diversity In Station L2supporting

confidence: 60%

Diversity of nifH gene sequences in the sediments of South China Sea

Wu¹

2011

Afr. J. Microbiol. Res.

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“…Since its publication, the PolF/ PolR primer pair has been used in a number of soil studies, including SYBR Green-based qPCR analyses (e.g. Park et al, 2007;Bowers et al, 2008;Coelho et al, 2009). In several studies covering various habitats, the authors detected either low numbers of cyanobacterial nifH sequences (Bauer et al, 2008;Terakado-Tonooka et al, 2008) or no cyanobacterial nifH sequences at all (Coelho et al, 2009;Lau & Pointing, 2009).…”

Section: Discussionmentioning

confidence: 99%

NifH expression by five groups of phototrophs compared with nitrogenase activity in coastal microbial mats

Severin

Stal

2010

FEMS Microbiology Ecology

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Diazotrophic (nitrogen-fixing) Cyanobacteria are often structurally dominant in coastal microbial mats but diazotrophs from other bacterial lineages are also present and active. The expression of nifH by four nonheterocystous Cyanobacteria and one member of the Gammaproteobacteria was followed over a 24-h cycle using quantitative reverse transcriptase-PCR. Daily nifH expression patterns were compared with the actual nitrogenase activity (NA) of the entire mat community. Lyngbya sp. was identified as the dominant cyanobacterium but, although recognized as a diazotroph, its cell-specific and abundance-related nifH expression was low. Unexpectedly, the other three cyanobacterial phylotypes dominated community nifH expression at all stations. Also, the gammaproteobacterium showed high levels of cell-specific nifH expression but its nifH copy number was low. Its contribution to the whole community nifH expression was therefore low. These results indicate that there were varying levels of cell-specific expression of nifH in the different mat types and more so, varying contributions to the overall nifH expression by the different diazotrophs. Furthermore, NA did not follow nifH expression patterns.

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“…Analysis of a model pulp and paper wastewater facility that was dependent on nitrogen fixation showed a high density of nifH sequences, but with low diversity (Bowers et al 2008). A nifH gene was identified in a Microbacterium desert isolate that is able to fix nitrogen under aerobic conditions (Gonzalez-Ruiz et al 2006), and Fani and Pini (2006) detected nifH sequences in two Arthrobacter isolates from Antarctic.…”

Section: Non-frankia Nitrogen-fixing Actinobacteriamentioning

confidence: 96%

Phylogenetic perspectives of nitrogen-fixing actinobacteria

et al. 2011

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It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. During the last decade, Mycobacterium flavum, Corynebacterium autotrophicum and a fluorescent Arthrobacter sp. have been reported to have nitrogenase activity, but these studies have not been further verified. Additional reports of nitrogen fixation by Agromyces, Microbacterium, Corynebacterium and Micromonospora isolated from root nodules of leguminous and actinorhizal plants have increased. For several actinobacteria, nitrogen fixation was demonstrated by the ability to grow on nitrogen-free medium, acetylene reduction activity, 15N isotope dilution analysis and identification of a nifH gene via PCR amplification. Moreover, the analyses of draft genome sequences of actinobacteria including Slackia exigua, Rothia mucilaginosa and Gordonibacter pamelaeae have also revealed the presence of nifH-like sequences. Whether these nifH sequences are associated with effective nitrogen fixation in these actinobacteria taxa has not yet been demonstrated. These genes may be vertically or horizontally transferred and be silent sequences. These ideas merit further investigation. This minireview presents a phylogenetic comparison of nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of this catalytic ability among actinobacteria.

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Composition of nifH in a wastewater treatment system reliant on N2 fixation

Cited by 22 publications

References 27 publications

Diversity of nifH gene sequences in the sediments of South China Sea

Diversity of nifH gene sequences in the sediments of South China Sea

NifH expression by five groups of phototrophs compared with nitrogenase activity in coastal microbial mats

Phylogenetic perspectives of nitrogen-fixing actinobacteria

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