Composition of Fungal Flora in Raw Refinery Effluent, Effluent Retention Pond and a Treated Effluent Recipient River

Machido, D. A.; Yakubu, SE; Ezeonuegbu, BA

doi:10.4314/jasem.v18i4.5

Cited by 9 publications

(10 citation statements)

References 3 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The value of BOD is considerably high, indicating that bacteria in the effluent are rapidly depleting the oxygen as the organic waste is being degraded. This is consistent with other studies on the isolation of hydrocarbon degrading microbes from refinery effluent (Machido et al, 2014;Stanley et al, 2017). United State Environmental Protection Agency (USEPA).…”

Section: Discussionsupporting

confidence: 93%

Diesel-Degrading Potential of Pseudomonas putida Isolated from Effluent of a Petroleum Refinery in Nigeria

Saidu¹,

Ameh²,

Atta³

2019

UJMR

View full text Add to dashboard Cite

The contamination of soil and groundwater by hazardous chemicals has become a major concern due to the associated risks to human health and the environment. The ability of Pseudomonas putida isolated from a petroleum refinery effluent to degrade diesel was assessed in this study. The effluent sample was collected from the treatment plant in the Kaduna Refining and Petrochemical Company (K.R.P.C), Nigeria. The physicochemical properties and heavy metal content of the effluent was determined, and three strains of Pseudomonas putida were identified among the bacteria isolated using conventional biochemical and phenotypic tests. The strain showing the highest degradation potential after screening was selected for the final biodegradation studies. The ability of the selected strain of Pseudomonas putida (C15a) to utilize the hydrocarbons in diesel was assessed over a period of eighteen days, and monitored on a three-day interval by evaluating the pH, hydrocarbon utilizing bacterial count and oil and grease content. It was observed that the organism was able to utilize diesel for its metabolic needs as shown by the increase in hydrocarbon utilizing bacterial (HUB) count and corresponding decrease in oil and grease content as well as pH. The highest hydrocarbon utilizing bacterial count was observed at day 15 (1.85 x 10 7 CFU/mL) with highest hydrocarbon degradation occurring at day 18 (98.3%). The strain of Pseudomonas putida (C15a) isolated in this study can be used as a candidate for further bioremediation studies on petroleum.

show abstract

Section: Discussionsupporting

confidence: 93%

Diesel-Degrading Potential of Pseudomonas putida Isolated from Effluent of a Petroleum Refinery in Nigeria

Saidu¹,

Ameh²,

Atta³

2019

UJMR

View full text Add to dashboard Cite

show abstract

“…Of the secondary metabolites analyzed by UHPLC-MS, five were selected for further discussion (Figure 2(1)) based on dispersion and distance from other compounds following principal component anal- Trichoderma, Rhizoctonia, Nigrospora, and Chaetophoma have previously been isolated from raw refinery effluents (Machido, Yakubu, & Ezeonuegbu, 2014). However, to our knowledge, this is the first study to not only report on the existence of fungi in carwash effluents but to also characterize such fungi and profile their secondary metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…Numerous studies have reported the existence of fungi in pollution‐stressed and extreme environments (Aguilera, ; Aristi et al., ; Cantrell, Casillas‐Martínez, & Molina, ; Cantrell et al., ). Aspergillus , Penicillium , Curvularia , Fusarium , Microsporum , Trichoderma , Rhizoctonia , Nigrospora , and Chaetophoma have previously been isolated from raw refinery effluents (Machido, Yakubu, & Ezeonuegbu, ). However, to our knowledge, this is the first study to not only report on the existence of fungi in carwash effluents but to also characterize such fungi and profile their secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

Potential biotechnological capabilities of cultivable mycobiota from carwash effluents

Sibanda¹,

Selvarajan²,

Tekere³

et al. 2017

MicrobiologyOpen

View full text Add to dashboard Cite

Urban life has created man‐made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and molecular techniques, fungi from carwash effluents was characterized for production of lipase and cellulase enzymes, nonpolar and polar biotechnologically relevant secondary metabolites and hydrocarbon utilization. The isolated fungal strains belonged to the genera Alternaria, Cladosporium, Penicillium, Peyronellaea, Rhizopus, Spegazzinia, Trichoderma, Ulocladium and Yarrowia. Sixty‐six percent (66%) of the fungal isolates were found to be able to metabolize naphthalene and benzanthracene, showing potential for application in bioremediation of hydrocarbon polluted sites. Lipase production by the isolates Penicillium sp. BPS3 (2.61 U/ml), Trichoderma sp. BPS9 (2.01 U/ml), Rhizopus sp. CAL1 (2.05 U/ml), Penicillium sp. PCW1 (2.99 U/ml) and Penicillium sp. SAS1 (2.16 U/ml) compared well with previously recorded lipase production levels by other fungi. The highest producers of cellulase were Penicillium sp. SAS1 (12.10 U/ml), Peyronella sp. CAW5 (4.49 U/ml) and Cladosporium sp. SAS3 (4.07 U/ml), although these activities were lower than previously reported levels. GC‐MS analysis of the fungal secondary metabolites resulted in identification of 572 compounds, including azulene, methanamine, N‐pentylidene, metoclopramide, and mepivacaine while compounds determined by UHPLC‐MS included 10‐undecen‐1‐ol, piquerol A, 10‐undecyn‐1‐ol, cyclo(leucylprolyl) and rac‐etomidate. These compounds were previously determined to have various activities including anticancer, antibacterial, antifungal, antihypertensive, antidiabetic and anti‐inflammatory properties. The study demonstrated that fungi from carwash effluents are natural sources of some biotechnologically important products.

show abstract

“…Study Site: The site for this study was the Kaduna Refinery and Petrochemical Company (KRPC) discharge point located at Chikun Local Government Area of Kaduna State, Nigeria. The refinery occupies an area of 2.9 square kilometres and is located on an undulating land about 700 metres above sea level, as described by Machido et al (2014).…”

Section: Methodsmentioning

confidence: 99%

Screening of Fungi Isolates from Kaduna Refinery Effluent and Romi River and Their Potential for Bioremediation

Obukohwo¹,

Vantsawa²,

Dibal³

et al. 2020

jasem

View full text Add to dashboard Cite

The operation of Kaduna Refinery and Petrochemical Company (KRPC) has increased effluent generation with consequent effects on water quality and habitat since it is discharged into nearby receiving water body. These effluents contain heavy metals and other toxicants. Samples were collected from the effluent discharge point of the Kaduna Refinery and Petrochemical Company (KRPC) and from Romi River located at Kaduna South Local Government Area of Kaduna state. Standard methods were used to analyze the physicochemical parameters and heavy metals of the effluents. A total of 14 fungi isolates were identified from the samples. These fungi isolates were screened for their bioremediation potential on some toxic components in refinery effluent and were identified using molecular techniques. Four fungi isolates (Chrysosporium tropicum, Aspergillus flavus, Aspergillus niger and Rhizopus oryzae) were selected for bioremediation. Carbon (IV) oxide evolution increased progressively during the period of bioremediation. There was a noticeable decline in the phenol, lead, cadmium and nickel in the entire bioremediation medium. There was a positive correlation between phenol and cadmium with a coefficient of 0.969. Consortia of fungi isolated from the refinery effluent and Romi River samples were effective in the bioremediation of refinery effluent. The mixed consortium of four fungi showed the most efficacies in the bioremediation of refinery effluent in terms of phenol, oil and grease, cadmium, lead and cadmium reduction. Kaduna Refinery and Petrochemical Corporation (KRPC) should adopt bioremediation as one of the techniques in treating effluents before being discharged into receiving water bodies Keywords: Effluents, Fungi, heavy metal, bioremediation.

show abstract

Composition of Fungal Flora in Raw Refinery Effluent, Effluent Retention Pond and a Treated Effluent Recipient River

Cited by 9 publications

References 3 publications

Diesel-Degrading Potential of Pseudomonas putida Isolated from Effluent of a Petroleum Refinery in Nigeria

Diesel-Degrading Potential of Pseudomonas putida Isolated from Effluent of a Petroleum Refinery in Nigeria

Potential biotechnological capabilities of cultivable mycobiota from carwash effluents

Screening of Fungi Isolates from Kaduna Refinery Effluent and Romi River and Their Potential for Bioremediation

Contact Info

Product

Resources

About