Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility

Liu, Jiamei; Li, Wanhong; Weng, Xiuxiu; Yue, Xiangpeng; Li, Fadi

doi:10.3390/ani12233302

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…Although studies on its function in female reproduction are lacking, several studies have demonstrated its fundamental role in male fertility in mammals (Zadravec et al, 2011;Olia Bagheri et al, 2021;Castellini et al, 2022;Liu et al, 2022). Regulation of pH also plays a role in sperm survival and activity in the UVJ of birds (Holm et al, 1996;Holm and Wishart, 1998).…”

Section: Discussionmentioning

confidence: 99%

Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens

Kosonsiriluk,

Reed,

Noll

et al. 2023

Front. Physiol.

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Artificial insemination is a standard practice in the turkey breeder industry to ensure the production of fertile eggs. Even though hens are inseminated on a weekly basis, their fertility tends to decline after a few weeks of production. Avian species have a specialized structures called sperm storage tubules (SSTs), located in the uterovaginal junction (UVJ) of the oviduct. The ability of SSTs to store sperm is directly correlated with the fertility of the hen. The objective of the study was to examine changes in the transcriptome of the turkey hen’s UVJ in response to the presence of sperm at three key stages of production. We hypothesized that repeated and prolonged exposure to sperm would alter the transcriptome of the UVJ. Samples were collected from virgin hens prior to the onset of lay, as well as from sham-inseminated (extender only) and semen-inseminated hens at early lay, peak lay, and late lay. Gene expression profiling of the UVJ was examined, and a differential expression analysis was conducted through pairwise comparisons between semen- and sham-inseminated groups at each production stage and across production stages. In the early laying stage, no significant gene expression changes were found between semen- and sham-inseminated groups. However, at peak lay, genes related to lipid biosynthesis, Wnt signaling, cell proliferation, and O-glycan biosynthesis were upregulated in the semen group, while the immune response and cytokine-cytokine receptor interaction were downregulated. In the late lay stage, the transcription pathway was upregulated in the semen group, whereas the translation pathway was downregulated. The local immune response that was suppressed during peak lay was increased at the late laying stage. In the semen-inseminated group, the UVJ exhibited advanced aging at the late laying stage, evidenced by reduced telomere maintenance and translation processes. The results from this study provide valuable insights into the alteration of the UVJ function in response to the presence of sperm at different stages of production and throughout the production cycle. Targeting the modulation of local immune response and addressing aging processes after peak production could potentially prevent or delay the decline in fertility of turkey breeder hens.

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Section: Discussionmentioning

confidence: 99%

Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens

Kosonsiriluk,

Reed,

Noll

et al. 2023

Front. Physiol.

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“…In order to improve the productivity of Tan sheep meat, the Tan sheep Breeding Project Team introduced excellent Hu sheep for crossbreeding, in order to improve the production performance of Tan sheep without affecting the quality and flavor of Tan sheep. The Hu sheep is extensively reared for its high litter size (5,6), and its muscle growth rate is faster than that of Tan sheep (7)(8)(9). Their hybrid offspring also grew faster than Tan sheep (data not published).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome DNA methylation profiling reveals epigenetic signatures in developing muscle in Tan and Hu sheep and their offspring

et al. 2023

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IntroductionThe Tan sheep is a popular local breed in China because of its tenderness and flavor. The Hu sheep breed is also famous for its high litter size, and its muscle growth rate is faster than that of Tan sheep. However, the epigenetic mechanism behind these muscle-related phenotypes is unknown.MethodsIn this study, the longissimus dorsi tissue from 18 6 month-old Tan sheep, Hu sheep, and Tan-Hu F2 generation (6 sheep per population) were collected. After genomic DNA extraction, whole-genome bisulfite sequencing (WGBS) and bioinformatics analysis were performed to construct genome-wide DNA methylome maps for the Tan sheep, Hu sheep and their Tan-Hu F2 generation.ResultsDistinct genome-wide DNA methylation patterns were observed between Tan sheep and Hu sheep. Moreover, DNA methylated regions were significantly increased in the skeletal muscle from Tan sheep vs. the F2 generation compared to the Hu sheep vs. F2 generation and the Tan sheep vs. Hu sheep. Compared with Hu sheep, the methylation levels of actin alpha 1 (ACTA1), myosin heavy chain 11 (MYH11), Wiskott-Aldrich syndrome protein (WAS), vav guanine nucleotide exchange factor 1 (VAV1), fibronectin 1 (FN1) and Rho-associated protein kinase 2 (ROCK2) genes were markedly distinct in the Tan sheep. Furthermore, Gene Ontology analysis indicated that these genes were involved in myotube differentiation, myotube cell development, smooth muscle cell differentiation and striated muscle cell differentiation.ConclusionThe findings from this study, in addition to data from previous research, demonstrated that the ACTA1, MYH11, WAS, VAV1, FN1, and ROCK2 genes may exert regulatory effects on muscle development.

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Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility

Cited by 2 publications

References 29 publications

Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens

Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens

Whole-genome DNA methylation profiling reveals epigenetic signatures in developing muscle in Tan and Hu sheep and their offspring

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