Composition of Carotenoids in Scenedesmus protuberans: Application of Chromatographic and Spectroscopic Methods

Erdoğan, Ayşegül; Çağır, Ali; Dalay, Meltem Conk; Eroğlu, Ahmet E.

doi:10.1007/s12161-015-0088-8

Cited by 22 publications

(12 citation statements)

References 33 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major identified pigment in the Ss10 extract was the lutein. These findings are in agreement with those by Erdoga et al 2015 [65]. Additionally, Sánchez et al 2008 [28] demonstrated that Scenedesmus almeriensis stressed with 5 g/L of NaCl presented a high content of lutein in its biomass composition, as a kind of adaptation to the cultivation conditions.…”

Section: Discussionsupporting

confidence: 91%

“…The ionization technique and the composition of the used mobile phase influence the fragments' pattern observed in the mass spectra of the pigment [64]. In this study, identification of carotenoids present in the Ss10 extract was carried out using HPLC-MS, by comparing our data of UV-VIS absorption maxima (λ max ) and molecular ions of each LC peak (MI) with other publications [65,66]. Our data showed that the Ss10 extract was rich in xanthophylls eluted from 22 to 43 min, and also contained carotene and chlorophyll pigments ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.

et al. 2020

View full text Add to dashboard Cite

Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of Scenedesmus sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in Scenedesmus sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that Scenedesmus sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The extraction procedure was adapted from Erdoğan et al, (2015). The main difference was that the saponification procedure was not performed in this study since the saponification causes fucoxanthin into fucoxanthinol under severe basic conditions (Yonekura et al, 2010;Haugan et al, 1992).…”

Section: Extraction Of Fucoxanthin From Cylindrotheca Closteriummentioning

confidence: 99%

Untitled

Erdoğan¹,

Demirel²,

Dalay³

et al. 2016

Turk. J. Fish. Aquat. Sci.

Self Cite

View full text Add to dashboard Cite

Production of fucoxanthin by diatoms has become an alternative research area due to their low cost, convenience and diversity. The fucoxanthin content of Cylindrotheca closterium and its enhancement by altering the cultivation conditions via oxidative stress were investigated in this study. For this purpose, the extraction parameters were optimized and the highest fucoxanthin concentration (6.58 mg g -1 ) was achieved within 15.0 minutes at 40 o C. Then, this yield reached to 10.15 mg g -1 in the presence of NaOCl and Fe 2+ . It is worth noting that, this is the first time that the effect of oxidative stress on fucoxanthin production in diatom has been studied according to our knowledge. Therefore, the results of this study and the discussion about the mechanisms can be a reference for the enrichment of fucoxanthin from other diatoms.Keywords: Fucoxanthin; Cylindrotheca closterium, oxidative stress; diatom; extraction. Cylindrotheca closterium'un Fukoksantin İçeriği ve Oksidatif Stres ile Arttırılması ÖzetFukoksantinin diatomlar tarafından üretimi düşük maliyet, kolaylık ve çeşitlilik nedeniyle alternatif bir araştırma alanı haline gelmiştir. Bu çalışmada, Cylindrotheca closterium'da bulunan fukoksantin miktarı ve kültür koşullarına uygulanan oksidatif stresin, bu içeriği nasıl arttırdığı araştırılmıştır. Bu amaçla,ekstraksiyon parametreleri optimize edilmiş ve en yüksek fukoksantin derişimi ( 6,58 mg g -1 ) 40 o C''de ve 15,0 dakika içinde elde edilmiştir. Daha sonra, fukoksantin miktarı, NaOCl ve Fe 2+ varlığında 10,15 mg g -1 a kadar ulaşmıştır. Bu çalışma ile ilk kez bir diatomun kültür koşullarına uygulanan oksitafis stres ile fukoksantin miktarındaki değişim gözlenmiştir. Bu nedenle; söz konusu çalışma, sonuçları ve mekanizmalar hakkında yapılan değerlendirmeler ile fukoksantinin diğer diatomlarda da zenginleştirilmesi açısından bir referans olabilir.

show abstract

“…The gradient system developed by Erdoğan et al (2015) was applied for the separation of carotenoids in the selected (2) all-trans-neoxanthin, (3) all-trans-violaxanthin, (4) 9-cis-violaxanthin, (5) all-trans-lutein, (6) 9-or 9′-cis-lutein, (7) trans-β-apo-8′-carotenal, (8) all-trans-β,ɛ-carotene, (9) all-trans-β,β-carotene, (10) 9-cis-β,β-carotene Britton et al 2004 b trans-β-apo-8'-Carotenal c Spectral fine structure: ratio of the longest wavelength absorption peak (designated as III) and that of the middle absorption peak (designated as II) cyanobacteria. Internal standard calibration method was used for the determination of carotenoids.…”

Section: Resultsmentioning

confidence: 99%

“…were performed according to the procedure reported in our previous study in which the method validation was also performed (Erdoğan et al 2015).…”

Section: Preparation Of Standard Carotenoid Solutionsmentioning

confidence: 99%

Carotenoid Profile in Prochlorococcus sp. and Enrichment of Lutein Using Different Nitrogen Sources

et al. 2016

Self Cite

View full text Add to dashboard Cite

Various carotenoids of the cyanobacterium P r o c h l o r o c o c c u s s p . a r e i d e n t i f i e d u s i n g chromatographic/spectroscopic techniques and quantified using HPLC-DAD. In the present study, β-apo-8′-carotenal was used as internal standard. Identification of carotenoids was carried out by comparing the retention time, absorption spectra, and mass spectra of unknown peaks with reference standards. All-trans-lutein was found to be the major carotenoid in this cyanobacterium, and, therefore, algal productivity and the potential for lutein accumulation were analyzed as a function of different nitrogen sources such as nitrate, nitrite, ammonia, and urea for cultivation. Among them, urea clearly led to the best lutein accumulation. According to the experimental evidence, lutein increased from 2.54 to 3.34 mg g −1 in the cyanobacteria when urea was used as the nitrogen source.

show abstract

Composition of Carotenoids in Scenedesmus protuberans: Application of Chromatographic and Spectroscopic Methods

Cited by 22 publications

References 33 publications

Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.

Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.

Untitled

Carotenoid Profile in Prochlorococcus sp. and Enrichment of Lutein Using Different Nitrogen Sources

Contact Info

Product

Resources

About