Composition of bacterial and archaeal communities in freshwater sediments with different contamination levels (Lake Geneva, Switzerland)

Haller, Laurence; Tonolla, Mauro; Zopfi, Jakob; Peduzzi, Raffaele; Wildi, Walter; Poté, John

doi:10.1016/j.watres.2010.11.018

Cited by 186 publications

(120 citation statements)

References 58 publications

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“…This confirms previous clone library-based observations from Vidy Bay (Haller et al, 2011). The communities at the most strongly ARG-contaminated sites STEP and EG2 were highly similar to some untreated wastewater communities.…”

Section: Spatial Trends In Arg Abundancesupporting

confidence: 89%

“…The observed correlation can be explained by the parallel release of both types of contaminants from the WWTP. (b) The impact of the WWTP discharge on the deposition of fecal indicator bacteria and contrasting bacterial community composition has been shown previously (Haller et al, 2011). (c) Our analysis of community similarity using ARISA (Figure 4) confirmed a distinct community composition of ARG-contaminated sediments in the vicinity of the discharge pipe that, furthermore, exhibits a high similarity to sewage communities (Figure 4a), supporting the notion that bacteria from these environments are deposited in the sediments.…”

Section: Direct and Indirect Causes Of Arg Contaminationsupporting

confidence: 85%

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Wastewater as a point source of antibiotic-resistance genes in the sediment of a freshwater lake

2014

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Antibiotic-resistance genes (ARGs) are currently discussed as emerging environmental contaminants. Hospital and municipal sewage are important sources of ARGs for the receiving freshwater bodies. We investigated the spatial distribution of different ARGs (sul1, sul2, tet(B), tet(M), tet(W) and qnrA) in freshwater lake sediments in the vicinity of a point source of treated wastewater. ARG contamination of Vidy Bay, Lake Geneva, Switzerland was quantified using real-time PCR and compared with total mercury (THg), a frequently particle-bound inorganic contaminant with known natural background levels. Two-dimensional mapping of the investigated contaminants in lake sediments with geostatistical tools revealed total and relative abundance of ARGs in close proximity of the sewage discharge point were up to 200-fold above levels measured at a remote reference site (center of the lake) and decreased exponentially with distance. Similar trends were observed in the spatial distribution of different ARGs, whereas distributions of ARGs and THg were only moderately correlated, indicating differences in the transport and fate of these pollutants or additional sources of ARG contamination. The spatial pattern of ARG contamination and supporting data suggest that deposition of particle-associated wastewater bacteria rather than co-selection by, for example, heavy metals was the main cause of sediment ARG contamination.

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Section: Spatial Trends In Arg Abundancesupporting

confidence: 89%

Section: Direct and Indirect Causes Of Arg Contaminationsupporting

confidence: 85%

Wastewater as a point source of antibiotic-resistance genes in the sediment of a freshwater lake

2014

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“…On the contrary, other studies found a significant difference in microbial diversity between sediments with and without heavy metal contamination (Haller et al 2011). Another important consideration is metal bioavailability in the environment, which is influenced by the species of metals and also the possible sorbents (e.g., minerals, organics) in the environment (e.g., Sparks 2005).…”

Section: Introductionmentioning

confidence: 96%

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

Horton

Rui

et al. 2015

Ann Microbiol

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Heavy metal concentrations within freshwater systems can increase as a result of anthropogenic inputs, which in turn can influence microbial community composition and community function. In this study, lake sediments collected from two historically heavy metal impacted geographical regions [Poyang Lake, People's Republic of China (PRC), and sites adjacent to the Muskegon Watershed, MI (USA)] were analyzed for total and available heavy metals (Cd, Cr, Cu, Pb, and Zn), as well as microbial community structure using highthroughput sequencing technology. In PRC tributaries leading into Poyang Lake, Zn was found to be the most available metal species. In USA sites, available Cd was higher in Mona Lake sites than alternate USA sites. In both regions, results indicate a weak influence of metals on microbial alpha and beta diversity, but strong positive and negative correlations of available heavy metals with specific taxonomic groups of bacteria and archaea. Further, individual metal species impacted microbial groups and operational taxonomic units differently, suggesting that individual metal species are important in determining which microbial groups and species prevail under heavy metal stress, particularly in the case of bioavailable metals. In PRC, several taxonomic groups were correlated positively with Zn, including Actinobacteria, Bacteroidetes, Proteobacteria, and Verrucomicrobia. Geobacter and Pedosphaerales were both correlated positively to Cr in the same sites, while other correlations with microbial groups were negative. In sites from the USA, Deltaproteobacteria were correlated positively with Cd, while Betaproteobacteria were negatively correlated, suggesting that Cd resistance may differ at the level of Class within Proteobacteria. This study highlights the importance of analyzing available metals when examining the impact of this disturbance on microbial community structure within aquatic sediments.

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“…DGGE has been applied in numerous research studies involving the assessment of microbial diversity of rivers, streams, lakes and sediment, to determine the water quality of the resource (De Figueiredo et al, 2010;Essahale et al, 2010;De Figueiredo et al, 2011;Haller et al, 2011). This method opened up new avenues of research on the diversity of microorganisms present in complex aquatic environments.…”

Section: Introductionmentioning

confidence: 99%

The impact of physico-chemical water quality parameters on bacterial diversity in the Vaal River, South Africa

Jordaan¹,

Bezuidenhout²

2013

WSA

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This study aimed to identify bacterial community structures in the Vaal River using PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) and high-throughput sequencing. The impact of physico-chemical characteristics on bacterial structures was investigated through multivariate analysis. Samples were collected from 4 sampling stations along the Upper Vaal River during winter (June 2009) and summer (December 2010). Physico-chemical analysis was conducted on-site. Additional physico-chemical data were obtained from statutory bodies. DNA was directly isolated from water samples and PCR amplified using universal bacterial primer pairs. PCR products were subjected to DGGE fingerprinting and high-throughput sequencing, followed by Shannon-Weaver diversity calculations, cluster analysis and multivariate analysis. Physico-chemical parameters did not exceed the prescribed South African water quality standards for domestic use, aquatic ecosystems, livestock watering and irrigation. DGGE banding patterns revealed similar bacterial community structures for 3 of the 4 sampling stations. PCA and RDA indicated that pH, water temperature and inorganic nutrient concentrations could be used to explain changes in bacterial community structures. High-throughput sequencing data showed that bacterial assemblages were dominated by common freshwater groups: Cyanobacteria, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria. Other freshwater phyla such as Deltaproteobacteria, Epsilonbacteria, Acidobacteria, Verrucomicrobia, Firmicutes, Fusobacteria, Flavobacteria and Fibrobacteres were found in low proportions. This study provides an overview of the dominant bacterial groups in the Upper Vaal River and the impact of environmental changes on bacterial diversity.

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Composition of bacterial and archaeal communities in freshwater sediments with different contamination levels (Lake Geneva, Switzerland)

Cited by 186 publications

References 58 publications

Wastewater as a point source of antibiotic-resistance genes in the sediment of a freshwater lake

Wastewater as a point source of antibiotic-resistance genes in the sediment of a freshwater lake

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

The impact of physico-chemical water quality parameters on bacterial diversity in the Vaal River, South Africa

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