Composition of Aqueous Solutions Containing Sodium Glycocholate and Glycodeoxycholate

Bottari, Emilio; Festa, Maria Rosa

doi:10.1002/adic.200590092

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…Conversely, intermediate micellar size, between those of pure NaGDC and NaTDC, are obtained when the mixture of two dihydroxy salts are considered . Electromotive force (EMF) measurements carried out on systems formed by both NaGC and NaGDC confirmed the formation of two-component aggregates, containing both the bile salt anions, the glycocholate aggregation numbers being even and those of glycodeoxycholate being always a multiple of three …”

Section: Introductionmentioning

confidence: 91%

Sodium Glycodeoxycholate and Glycocholate Mixed Aggregates in Gas and Solution Phases

et al. 2009

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This paper deals with electrospray ionization mass spectrometry (ESIMS), small-angle X-ray scattering (SAXS), and dynamic light scattering (DLS) measurements in order to provide information on the existence, aggregation, composition, and structure of the two-component aggregates of sodium glycocholate (NaGC) and sodium glycodeoxycholate (NaGDC) in the gas and solution phases. Five samples, containing 100% NaGC and 100% NaGDC, and NaGDC/NaGC molar ratios of 3 (75D), 1 (50D), and 1/3 (25D), have been analyzed by ESIMS in positive-ion detection mode starting from 10(-3) and 10(-2) M total bile salt concentration in aqueous solutions. Generally, dimers or trimers prevail in the 100% NaGC or NaGDC samples, respectively, as observed in the preceding one-component ESIMS measurements and in agreement with the proposed micellar aggregate structures in aqueous solution. Moreover, it is observed that the composition of multimers in the samples 75D, 50D, and 25D deviates from the one expected on the basis of a random association of the monomers, the NaGDC contribution generally prevailing on the NaGC one. It happens also under the same percentage condition (50D sample), in agreement with a greater aggregation ability of NaGDC with respect to NaGC. SAXS and DLS data were recorded on six samples containing a NaGC+NaGDC 40 mM total concentration, one bile salt having 40, 32, 24, 16, 8, and 0 mM concentration and the other the complementary one, keeping constant the NaCl concentration (0.6 M). The NaGDC 40 mM sample presents SAXS curves in agreement with a cylindrical shape of the aggregates as shown in a previous paper. For the bile salt mixtures, the progressive decrease of the sizes and change of the aggregate morphology, toward a globular-like geometry, are observed by increasing the NaGC fraction, thus confirming the hypothesis about the ability of trihydroxy salts to inhibit the growth of dihydroxy salt aggregates. Fits on the basis of cylindrical model can be accomplished for all the SAXS spectra, however, when the extracted cylinder parameters are used to estimate theoretical hydrodynamic radii a reasonable agreement is obtained only for the samples at high fraction of NaGDC (NaGDC>or=24 mM).

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Section: Introductionmentioning

confidence: 91%

Sodium Glycodeoxycholate and Glycocholate Mixed Aggregates in Gas and Solution Phases

et al. 2009

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“…Vice versa this trend can be explained by assuming, as shown previously, [17][18][19][20][21] that sodium ions can associate, more or less strongly, with some bile salts.…”

Section: Discussionmentioning

confidence: 65%

Coulometry: A Fine Procedure to Determinate Acidity Constants of Slightly Soluble Acids

et al. 2010

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The flowing of constant direct current allows planning a coulometric method to determine solubility and acidity constants of slightly soluble acids. Coulometry combined with electromotive force (emf) measurements is applied to obtain such parameters for eight bile acids, simple and conjugated with glycine in solutions in real equilibrium and without formation of micellar aggregates. Results, obtained in different ionic media, (1.00, 0.50, and 0.15) mol.dm(-3) NaCl (i.e., in physiologic conditions), are compared with previous data, extrapolated from mixed solvents or obtained in oversaturated solutions. The proposed method is not difficult to perform, and it can be applied to other slightly soluble acids or in very low concentrations, without a complicated elaboration of the experimental data

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“…Hydrochloric acid, sodium chloride, sodium hydroxide, tetramethylammonium chloride, and tetramethylammonium hydroxide were prepared and analyzed as previously , described. Calcium(II) chloride (RP C. Erba) was used to prepare the calcium(II) bile salts and to prepare standards for AA spectrometry.…”

Section: Methodsmentioning

confidence: 99%

“…The influence of the ionic composition of the solution on the behavior of sodium glycocholate and cholate, gel formation, and protonation constants of taurocholate and taurodeoxycholate were investigated.…”

Section: Introductionmentioning

confidence: 99%

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Solubility and the Solubility Product of Some Calcium(II) Salts of Bile Acids

2011

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The solubility of calcium(II) salts of some salts of bile acids was investigated. Among the considered compounds, the slightly soluble salts were prepared by adding a moderate excess of calcium(II) chloride solution to each solution of the selected sodium bile salts. The obtained solids were analyzed to check their composition and the crystallization water content. Each solid compound was brought in contact at 25 degrees C with an aqueous solution (0.15 or 0.50) mol.dm(-3) NaCl or 0.50 mol.dm(-3) N(CH(3))(4)Cl, as the ionic medium. The obtained suspension was stirred until reaching equilibrium. The equilibrated mixture was analyzed for the free hydrogen ion concentration and for the calcium(II) concentration. Solubility and solubility products were determined for the calcium(II) salts of the following bile salts: cholate, deoxycholate, glycodeoxycholate, ursodeoxycholate, chenodeoxycholate, lithocholate, and dehydrocholate. Calcium(II) glycocholate, taurocholate, and taurodeoxycholate were soluble compounds. The solubility of the investigated salts followed the order: cholate > dehydrocholate > glycodeoxycholate > ursodeoxycholate > chenodeoxycholate > deoxycholate > lithocholate. The solubility in 0.50 mot. dm(-3) NaCl was higher than in 0.15 mol.dm(-3) NaCl, Concerning the contained OH groups the solubility order was the following: trihydroxycholate > dihydroxycholate > monohydroxycholate

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Composition of Aqueous Solutions Containing Sodium Glycocholate and Glycodeoxycholate

Cited by 4 publications

References 24 publications

Sodium Glycodeoxycholate and Glycocholate Mixed Aggregates in Gas and Solution Phases

Sodium Glycodeoxycholate and Glycocholate Mixed Aggregates in Gas and Solution Phases

Coulometry: A Fine Procedure to Determinate Acidity Constants of Slightly Soluble Acids

Solubility and the Solubility Product of Some Calcium(II) Salts of Bile Acids

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