Composition of antibodies against acidic and basic azoproteins

Lymphœdèmes

“…Analyses of several purified rabbit antibodies have been published (Smith et al, 1955;Fleischer et al, 1961;Askonas et al, 1960), and their amino acid contents were all very close to the figures quoted above for rabbit IgG (Crumpton and Wilkinson, 1963). Since only about 1% of the total molecule may be concerned in the antibody site (Karush, 1962), it was not surprising that no convincing differences were found even if amino acid sequence does vary with antibody specificity.…”

A Comparison of Fragments of Rabbit Antibodies and Normal γ-Globulin by the Peptide-Map Technique*

“…Such differences would quite possibly be limited to the combining areas of the antibodies, which are only a small fraction of the antibody molecule (Kabat, 1961). It is therefore not surprising that the comparison of several immunospecifically purified antibodies revealed no significant differences in their amino acid composition (Smith et al, 1955;Fleischer et al, 1961). On the other hand, Koshland and Englberger (1963) have recently reported small but significant differences in the amino acid composition of antibodies to two different haptenic groups.…”

Studies in Immunoglobulin Chemisty. I. Reinvestigation of the C-Terminal Amino Acids of 6.5S Rabbit and Human γ-Globulins*

“…Although our results for intact rabbit -globulin differ from those of Silman et al (1962) to a great extent, their observation that glycine is essentially the only Cterminal amino acid in fragment III obtained from this immunoglobulin is confirmed by the present findings regarding the C-terminal residues of the H chains. In addition, it should be noted that Fleischer et al (.1961) earlier obtained evidence that implicated aspartic acid as the C-terminal amino acid in two specific rabbit antibodies. In most prior investigations, some variation of the hydrazinolysis method was employed.…”