Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis

Dillon, Rod J.; Webster, Gordon; Weightman, Andrew J.; Dillon, Vivian M.; Blanford, Simon; Charnley, A.K.

doi:10.1016/j.jip.2007.09.010

Cited by 52 publications

(47 citation statements)

References 40 publications

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“…dominated the gut microbiota of two sympatric populations of Moroccan (Dociostaurus moroccanus) and Italian locusts (Calliptamus italicus) from Spain. However, sequence analysis of DGGE bands did not reveal evidence for a high proportion of unculturable bacteria and homologies suggested that bacterial species were principally Gammaproteobacteria from the family Enterobacteriaceae similar to those recorded previously in laboratory reared locusts (Dillon et al 2008).…”

Section: Introductionsupporting

confidence: 76%

“…All PCR reactions were conducted with a PTC-100 thermocycler (M.J.Research Ltd). DGGE analysis of PCR amplified products DGGE was conducted according to previously described methods (Webster et al 2002;Dillon et al 2008). DGGE marker was prepared from a selection of bacterial 16S rRNA gene products to enable gel to gel comparison.…”

Section: Bacteria and Growth Mediamentioning

confidence: 99%

“…The gut bacterial community from four species of feral locusts and grasshoppers determined by DGGE revealed an effect of phase polymorphism on gut bacterial diversity in brown locusts (Locusta pardalina) from South Africa (Dillon et al 2008). A single bacterial phylotype, closely related to Citrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of gut microbiota increases with aging and starvation in the desert locust

Dillon

Webster

Weightman

et al. 2009

Antonie van Leeuwenhoek

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Here we report the effects of starvation and insect age on the diversity of gut microbiota of adult desert locusts, Schistocerca gregaria, using denaturing gradient gel electrophoretic (DGGE) analysis of bacterial 16S rRNA genes. Sequencing of excised DGGE bands revealed the presence of only one potentially novel uncultured member of the Gammaproteobacteria in the guts of fed, starved, young or old locusts. Most of the 16S rRNA gene sequences were closely related to known cultured bacterial species. DGGE profiles suggested that bacterial diversity increased with insect age and did not provide evidence for a characteristic locust gut bacterial community. Starved insects are often more prone to disease, probably because they compromise on immune defence. However, the increased diversity of Gammaproteobacteria in starved locusts shown here may improve defence against enteric threats because of the role of gut bacteria in colonization resistance.

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Section: Introductionsupporting

confidence: 76%

Section: Bacteria and Growth Mediamentioning

confidence: 99%

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Diversity of gut microbiota increases with aging and starvation in the desert locust

Dillon

Webster

Weightman

et al. 2009

Antonie van Leeuwenhoek

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“…(Gokce et al 2010), Alcaligenes faecalis (Osborn et al 2002), Serratia marcescens (Rani et al 2009), Leucobacter sp. (Murrell et al 2003), Variovorax paradoxus (Dillon et al 2008), Corynebacterium glutamicum (Mrazek et al 2008) and Ochrobactrum sp. (Volf et al 2002) with insects has previously been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, V. paradoxus has only been isolated from grasshopper (Dillon et al 2008). In addition, insect pathogenic activity has never been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization and virulence of bacteria from Ostrinia nubilalis (Lepidoptera: Pyralidae)

et al. 2012

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AbstractIsolation, characterization and virulence of the culturable bacteria from entire tissues of larval Ostrinia nubilalis (Hübner) (Lepidoptera: Pyralidae) were studied to obtain new microbes for biological control. A total of 16 bacteria were isolated from living and dead larvae collected from different maize fields in the Eastern Black Sea Region of Turkey. The bacterial microbiota of O. nubilalis were identified as Pseudomonas aeruginosa (On1), Brevundimonas aurantiaca (On2), Chryseobacterium formosense (On3), Acinetobacter sp. (On4), Microbacterium thalassium (On5), Bacillus megaterium (On6), Serratia sp. (On7), Ochrobactrum sp. (On8), Variovorax paradoxus (On9), Corynebacterium glutamicum (On10), Paenibacillus sp. (On11), Alcaligenes faecalis (On12), Microbacterium testaceum (On13), Leucobacter sp. (On14), Leucobacter sp. (On15) and Serratia marcescens (On16) based on their morphological and biochemical characteristics. A partial sequence of the 16S rRNA gene was also determined to confirm strain identification. The highest insecticidal activities were obtained from P. aeruginosa On1 (80%), Serratia sp. On7 (60%), V. paradoxus On9 (50%) and S. marcescens On16 (50%) against larvae 14 days after treatment (p < 0.05). Also, the highest activity from previously isolated Bacillus species was observed from Bacillus thuringiensis subsp. tenebrionis Xd3 with 80% mortality within the same period (p < 0.05). Our results indicate that P. aeruginosa On1, Serratia sp. On7, V. paradoxus On9, S. marcescens On16 and B. thuringiensis subsp. tenebrionis Xd3 show potential for biocontrol of O. nubilalis.

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Metagenomic Approaches for Insect Symbionts

Chellappan

Ranjith

2021

Microbial Approaches for Insect Pest Management

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Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis

Cited by 52 publications

References 40 publications

Diversity of gut microbiota increases with aging and starvation in the desert locust

Diversity of gut microbiota increases with aging and starvation in the desert locust

Isolation, characterization and virulence of bacteria from Ostrinia nubilalis (Lepidoptera: Pyralidae)

Metagenomic Approaches for Insect Symbionts

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