Composition chimique de la paroi d'une levure du genre Torulopsis apres culture sur methanol et ethanol

Leleu, J. B.; Morilhat, J.P.; Bonaly, R.

doi:10.1016/s0031-9422(00)94353-x

Cited by 2 publications

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“…The structure and chemical composition of the yeast cell wall vary between strains (Bartnicki-Garcia, 1968) and with growth conditions (Power & Challinor, 1969;Bonaly et al, 1971 ;Leleu et al, 1977). Hasenclever & Mitchell (1964) showed that flocculation of yeast was directly related to mannan in the cell walls.…”

Section: Introductionmentioning

confidence: 99%

Acetolysis and 1H NMR studies on mannans isolated from very flocculent and weakly flocculent cells of Pichia pastoris IFP 206

Mbawala

Mahmood

Loppinet

et al. 1990

Journal of General Microbiology

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Growth of the yeast Pichiapasturis IFP 206 in methanol-and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. lH NMR analysis showed that both kinds of mannan contained a-1,2 and p-1,2 linkages. Two acetolysis conditions, combined with 'H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose sidechains with the following respective structures: Man; Manalj2Man; Manal-+2Manal+2Man; ManPl +2Manal+2Man; Man/31-+2Manpl-+2Manal+2Man; Manal-+2Man~l-+2Man~l+2Manal+2Man. Additionally the p-1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysislabile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.

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