Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast

Wahlström, Niklas; Nylander, Filip; Malmhäll-Bah, Eric; Sjövold, Karin; Edlund, Ulrica; Westman, Gunnar; Albers, Eva

doi:10.1016/j.carbpol.2020.115852

Cited by 69 publications

(60 citation statements)

References 38 publications

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“…It is known that many of the activities of ulvan are similar to those of heparin, but a study on ulvan from U. rigida found it to be inactive against the anti-thrombin pathway unless chemically sulfated (Adrien et al 2019). Heparin is mostly extracted from porcine mucosa at yields of 160-260 mg kg −1 (van der Meer et al 2017), which is several times lower than the levels of iduronic acid seen here and much lower than the ulvan content in U. fenestrata, which contains at least 18% dw of ulvan (Wahlström et al 2020). In a broad range of studies on U. lactuca, even higher ulvan contents up to 36% dw have been seen (Kidgell et al 2019).…”

Section: Discussionmentioning

confidence: 72%

Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

et al. 2020

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In recent years, the interest in using seaweed for the sustainable production of commodities has been increasing as seaweeds contain many potentially worthwhile compounds. Thus, the extraction and refining processes of interesting compounds from seaweeds is a hot research topic but has been found to have problems with profitability for novel applications. To increase the economic potential of refining seaweed biomass, the content of the compounds of interest should be maximized, which can potentially be achieved through optimization of cultivation conditions. In this study, we studied how the monosaccharide composition of the green seaweed species Ulva fenestrata is influenced by the abiotic factors; irradiance, temperature, nitrate, phosphate, and pCO2. It was evident that lower nitrate concentration and cultivation at elevated temperature increased monosaccharide contents. A 70% increase in iduronic acid and a 26% increase in rhamnose content were seen under elevated irradiance and temperature conditions, though the absolute differences in monosaccharide concentration were small. Irradiance and nitrate impacted the ratio between iduronic and rhamnose, which is an indicator of the ulvan structure. These results could potentially be utilized to coax the ulvan towards specific bioactivities, and thus have a considerable impact on a potential biorefinery centered around Ulva.

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Section: Discussionmentioning

confidence: 72%

Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

et al. 2020

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“…Glucuronic acid is a constituent of the cell wall polysaccharide ulvan, and is also found in glucuronan (Redouan et al, 2009). Additionally, for other constituents of ulvan, rhamnose, iduronic acid and xylose (Wahlström et al, 2020), content increased in the east coast samples ( Table 1). The ANOVA indicated effects on both iduronic acid and xylose when looking at the unadjusted p values, which were 0.05 and 0.07 for these components, respectively (Supplementary table S3).…”

Section: Biomass Compositionmentioning

confidence: 96%

Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

et al. 2020

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Macroalgal biomass has the potential to become an important source of chemicals and commodities in a future biorefinery. Currently, production of macroalgal biomass is expensive and the content of high-value compounds is often low. Therefore, in this study the biochemical composition of Ulva intestinalis along the Swedish west coast and the east coast up to Stockholm was assessed with the aim of determining how the content of potentially valuable compounds, such as rhamnose, iduronic acid and PUFAs, could be maximized by utilizing natural variation in the choice of marine cultivation site. Along the investigated coastline, the salinity dropped from 19.4‰ at high latitudes along the west coast to 5.4‰ at Stockholm. Nitrogen and phosphorus availability varied, while temperature was similar at all locations. The two major components of biomass, carbohydrates and ash, varied inversely with the highest content of ash in the west and carbohydrates in the east. In addition, total fatty acids were significantly higher in west coast samples at 3.2 g 100 g-1 dw, with a higher proportion of mono-and polyunsaturated fatty acids. Some healthbeneficial fatty acids were found, including EPA and DPA, at 10-50 mg 100 g-1 dw, respectively. The metal content and elemental composition varied widely, probably due to the influence of specific local conditions. The P content was correlated with the phosphorus concentration in waters at the locations. In PCA analysis, the monosaccharides constituting the cell wall polysaccharide ulvan were found to vary by geographical location, with higher levels possibly associated with lower salinities. However, only glucuronic acid differed significantly between sites. These results show the considerable geographical variability in the composition of Swedish U. intestinalis and suggest that different salinities could be used to create a lipid-or carbohydrate-rich biomass.

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“…The extraction procedure has several parameters such as pH, temperature, and mechanical movements that determine the amount of obtained extract and metal content [32]. Acid extraction causes almost complete desulfation of isolated ulvan while purifying enzymatic methods maintain a significant SO 3 substituent level [33]. Several Ulva sp.…”

Section: Extractionmentioning

confidence: 99%

“…Extraction with pure distilled water is the most widely used method [41,42]. This procedure provides ulvan that is glucuronic acid, glucose, arabinose, and xylose, with an average yield of 25-40% [22,33,43]. Another extraction method involves adding 0.05-1% hydrochloric acid (pH 1.5 to 2), with an average total yield of 15-45% [10,22,33].…”

Section: Extractionmentioning

confidence: 99%

“…This procedure provides ulvan that is glucuronic acid, glucose, arabinose, and xylose, with an average yield of 25-40% [22,33,43]. Another extraction method involves adding 0.05-1% hydrochloric acid (pH 1.5 to 2), with an average total yield of 15-45% [10,22,33]. Enzyme extraction utilizes onozula, pectinase macerozyme, α-amylase, and proteinase K, which are suspended in the buffer.…”

Section: Extractionmentioning

confidence: 99%

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Ulvan, a Polysaccharide from Macroalga Ulva sp.: A Review of Chemistry, Biological Activities and Potential for Food and Biomedical Applications

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The species of green macroalga belonging to the genus Ulva (family: Ulvaceae) are utilized in various fields, from food supplements to biomedical applications. Ulvan, a polysaccharide obtained from various Ulva species, has shown various biological activities, including antioxidant, anti-inflammatory, anticancer, antibacterial, and antiviral activities. To obtain the polysaccharide ulvan that can be utilized in various fields, it is necessary to understand the critical points that affect its physicochemical nature, the extraction procedures, and the mechanism of action for biological activities. This article discusses the physicochemical properties, extraction, isolation and characterization procedures and benefits in food and biomedical applications of ulvan. In conclusion, ulvan from Ulva sp. has the potential to be used as a therapeutic agent and also as an additional ingredient in the development of tissue engineering procedures.

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Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast

Cited by 69 publications

References 38 publications

Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

Ulvan, a Polysaccharide from Macroalga Ulva sp.: A Review of Chemistry, Biological Activities and Potential for Food and Biomedical Applications

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