Composition and Structure of Articular Cartilage

Poole, A. Robin; Kojima, Toshi; Yasuda, Tadashi; Mwale, Fackson; Kobayashi, Masahiko; Laverty, Sheila

doi:10.1097/00003086-200110001-00004

Cited by 541 publications

(461 citation statements)

References 32 publications

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“…40 This reflects the unique ultrastructure of cartilage ECM, which is composed of proteoglycans embedded into a network of different types of collagen fibrils. Under cyclic loading, the applied stress and resulting strain are not in phase.…”

Section: Determining Biomechanical Properties Of Cartilage Repair Tismentioning

confidence: 98%

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

et al. 2010

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Section: Determining Biomechanical Properties Of Cartilage Repair Tismentioning

confidence: 98%

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

et al. 2010

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“…There are numerous types of ECM in human tissues, which usually have multiple components and tissue-specific composition. Readers are directed to detailed reviews for types of ECM [9,90,95] and their tissue-specific composition [13,91,112]. As for the functions of ECM in tissues, they can be generally classified into five categories (Table 1).…”

Section: Analogous Functions Of Scaffolds and Extracellular Matrixmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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“…In addition to these macromolecules, collagen types VI, IX, XI; decorin; biglycan, cartilage oligomeric matrix protein (COMP); and fibromodulin are also present in minor concentrations (Muir, 1995;Poole et al, 2001). It is well known that damaged articular cartilage has poor intrinsic regenerative capacity.…”

Section: Introductionmentioning

confidence: 99%

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

Bosnakovski

Mizuno

Kim

et al. 2006

Biotech & Bioengineering

432

321

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Bone marrow mesenchymal stem cells (MSCs) are candidate cells for cartilage tissue engineering. This is due to their ability to undergo chondrogenic differentiation after extensive expansion in vitro and stimulation with various biomaterials in three-dimensional (3-D) systems. Collagen type II is one of the major components of the hyaline cartilage and plays a key role in maintaining chondrocyte function. This study aimed at analyzing the MSC chondrogenic response during culture in different types of extracellular matrix (ECM) with a focus on the influence of collagen type II on MSC chondrogenesis. Bovine MSCs were cultured in monolayer as well as in alginate and collagen type I and II hydrogels, in both serum free medium and medium supplemented with transforming growth factor (TGF) b1. Chondrogenic differentiation was detected after 3 days of culture in 3-D hydrogels, by examining the presence of glycosaminoglycan and newly synthesized collagen type II in the ECM. Differentiation was most prominent in cells cultured in collagen type II hydrogel, and it increased in a timedependent manner. The expression levels of the of chondrocyte specific genes: sox9, collagen type II, aggrecan, and COMP were measured by quantitative ''Real Time'' RT-PCR, and genes distribution in the hydrogel beads were localized by in situ hybridization. All genes were upregulated by the presence of collagen, particularly type II, in the ECM. Additionally, the chondrogenic influence of TGF b1 on MSCs cultured in collagenincorporated ECM was analyzed. TGF b1 and dexamethasone treatment in the presence of collagen type II provided more favorable conditions for expression of the chondrogenic phenotype. In this study, we demonstrated that collagen type II alone has the potential to induce and maintain MSC chondrogenesis, and prior interaction with TGF b1 to enhance the differentiation.

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Composition and Structure of Articular Cartilage

Cited by 541 publications

References 32 publications

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

Articular cartilage repair by genetically modified bone marrow aspirate in sheep

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

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