Fifteen remnants of natural vegetation of dry openings were grouped using Jaccard's coefficient and the unweighted pair group method and by principal coordinate analysis, revealing three major groups designated as limestone savanna, prairie, and sand barren. Analysis of presence, frequency and cover data from quadrats in 11 major associations gave the same result. Sites within the three major classes differed substantially in species presence, frequency and cover, and were not individually representative of the class. Each of the three vegetation classes had some unique species. The savannas, developing on fine textured soils shallowly overlying limestone bedrock, were dominated by shrubs including Cornus racemosa, Symphoricarpos alba and Rhus aromatica. The prairies were dominated by the prairie grasses Andropogon gerardii, Schizachyrium scoparium and Sorghastrum nutans and the shrubs Prunus virginiana, Ceanothus herbaceus and Rhus aromatica. Sand barrens occurred on soils with less organic matter than prairies and were dominated by Carex siccata, Carex tonsa, Ceanothus americanus and Pteridium aquilinum. Species present in all three major vegetation types included Anemone cylindrica, Carex siccata, Carex pensylvanica, Poa compressa and Poa pratensis. Approximately 40% of the prevalent species in Wisconsin prairie and savanna were present in equivalent vegetation types of the Trent valley, and numerous species present in the Trent sites were primarily western, some at their absolute eastern limit. There was also a well-defined southern floristic element. The sand barrens have less floristic affinity with the west. The western elements may have reached the area in early post-glacial times through migration eastward along a periglacial sidewalk or along the shores of post-glacial lakes or they may have moved eastward in a series of short dispersal steps between natural openings during the warmer and drier hypsithermal interval when open habitats were probably more widespread, or they may have been carried over great distances to the Trent valley by Indians. The limestone savanna was probably maintained by drought whereas a combination of drought and frequent fire were probably important in maintaining the prairies and sand barrens. Eight nationally and provincially rare species and numerous regionally rare species occur on the prairie sites, which are the easternmost in Canada. The limestone savannas and sand barrens are also rich in rare species, but to a lesser extent.