Composition and predictive functional analysis of bacterial communities inhabiting Chinese Cordyceps insight into conserved core microbiome

Abstract

Background Over the past few decades, most attention to Chinese Cordyceps-associated endogenous microorganism was focused on the fungal community that creates critical bioactive components. Bacterial community associated with Chinese Cordyceps has been previously described; however, most studies were only presenting direct comparisons in the Chinese Cordyceps and its microenvironments. In the current study, our objectives were to reveal the bacterial community structure composition and predict their function. Results We collected samples of Chinese Cordyceps from five sites located in the Qinghai-Tibet Plateau and used a high throughput sequencing method to compare Chinese Cordyceps-associated bacterial community composition and diversity quantitatively across sites. The results indicated that for the Chinese Cordyceps-associated bacterial community there is no single core microbiome, which was dominated by the both Proteobacteria and Actinobacteria. Predictive functional profiling suggested a location specific function pattern for Chinese Cordyceps and bacteria in the external mycelial cortices involved in the biosynthesis of active constituents. Conclusions This study is firstly used high throughput sequencing method to compare the bacterial communities inhabiting Chinese Cordyceps and its microhabitat and to reveal composition functional capabilities of the bacteria, which will accelerate the study of the functions of bacterial communities in the micro-ecological system of Chinese Cordyceps. Electronic supplementary material The online version of this article (10.1186/s12866-019-1472-0) contains supplementary material, which is available to authorized users.

Uncovering fungal community composition in natural habitat of Ophiocordyceps sinensis using high-throughput sequencing and culture-dependent approaches

“…Therefore, O. sinensis samples were collected at five sites from Golog Tibetan Autonomous Prefecture of Qinghai Province, which is a suitable geographical distribution of O. sinensis with high quality and yield. Although the limited sampling sites covered by this study possibly result in decreasing of credibility of the present results, considerable previous studies support our data and hypothesis [ 19 , 41 ].…”

Uncovering fungal community composition in natural habitat of Ophiocordyceps sinensis using high-throughput sequencing and culture-dependent approaches

“…Therefore, O. sinensis samples were collected at ve sites from Golog Tibetan Autonomous Prefecture of Qinghai Province, which is a suitable geographical distribution of O. sinensis with high quality and yield. Although the limited sampling sites covered by this study possibly result in decreasing of credibility of the present results, considerable previous studies support our data and hypothesis [19,41].…”

Endogenous bacteria inhabiting the Ophiocordyceps highlandensis during fruiting body development

“…Numerous researchers have studied the rich diversity of many endogenic Ophiocordyceps -inhabiting microbial communities [ 7 , 9 – 14 , 23 , 24 ]. These studies suggest that species belonging to the Ascomycota are the most abundant in the fruiting body and soil microhabitats, which is consistent with our results (taking up 70.3–90.1%).…”

“…Moreover, the available knowledge suggests that some fungal cultivation strategies rely on the exploitation of specialized host microbes during infection [ 8 ]. As a result, experiments were performed to investigate the associations of microbial communities with O. sinensis [ 9 – 14 ]. However, the abovementioned conclusions were drawn based on the investigation and comparison of the diversity of microorganisms between geographic regions or different tissues.…”