Composition and morphology of wear debris in failed uncemented total hip replacement

Shanbhag, AS; Jacobs, JJ; Glant, TT; Gilbert, Jeremy L.; Black, Jonathan; Jo, Galante

doi:10.1302/0301-620x.76b1.8300684

Cited by 372 publications

(233 citation statements)

References 26 publications

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“…Our study extends these results and shows for the first time that not only TiAlV-, mixed-, or PMMA-particles [35,40], but also polyethylene-particles induce an activation of NF-KB signalling and the TNFa-promoter. Numerous studies of wear debris from periprosthetic tissues have underlined the key role of PE-particles demonstrating that 70-90% of the recovered particles are submicrometer polyethylene-particles [12, 24,34,46,47]. Metal-on-polyethylene and ceramic-on-polyethylene components are the most common bearing surfaces used in joint replacement leading predominantly to generation of polyethylene-particles.…”

Section: Discussionmentioning

confidence: 99%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

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Wear particles are believed to induce periprosthetic inflammation which contributes to periprosthetic osteolysis. TNFu plays a pivotal role in the pathogenesis of this process. The molecular mechanisms leading to the development of periprosthetic inflammation with upregulated TNFa expression in monocytic cells in response to different wear particles have yet to be defined. 1n:this study we evaluated the effects of polyethylene-and TiAIV-particles on activation of NF-KB signalling pathways and TNFa biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis. THP-1 monocytic cells were differentiated to macrophage-like cells and exposed to LPS-detoxified polyethylene and prosthesis-derived TiAlV-particles. TNFa release was analyzed in culture supernatant by ELISA. NF-KB activation was examined by electrophoretic mobility shift assay (EMSA), and NF-KB target promoter activities including transactivation of the TNFa promoter were determined by luciferase reporter gene assays. Differentiated THP-1 macrophages were exposed to increasing numbers of particles for 0, 60, 180 and 360 min. Both, polyethylene-and TiAIV-particles induced a significant activation of both NF-KB and TNFa promoters at 180 min. A significant TNFu release was detected after 360 min exposure to polyethylene-and TiAlV-particles in a dose dependent manner. In comparison, LPS induced a much greater activation of NF-KB and TNFa promoters, and TNFa secretion into the supernatant was strongly induced. These results provide evidence that induction of the NF-KB signal transduction pathway in macrophages plays a major role in initiating and mediating the inflammatory response leading to periprosthetic osteolysis.

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Section: Discussionmentioning

confidence: 99%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

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“…The first protocol, originally developed at our research center in the 1990s [8], has been widely used [24,31,46,48] and is hereinafter referred to as the NaOH protocol. Briefly, samples were digested with 5 N NaOH, layered above a sucrose density gradient, and submitted to ultracentrifugation, and the floating particles were collected on a polycarbonate (PC) filter membrane (0.01 lm) for analysis via a field emission scanning electron microscope (FE-SEM) (Supra VP-40; Zeiss, Peabody, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Billi

Benya

Kavanaugh

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Background Numerous studies indicate highly crosslinked polyethylenes reduce the wear debris volume generated by hip arthroplasty acetabular liners. This, in turns, requires new methods to isolate and characterize them. Questions/purposes We describe a method for extracting polyethylene wear particles from bovine serum typically used in wear tests and for characterizing their size, distribution, and morphology.Methods Serum proteins were completely digested using an optimized enzymatic digestion method that prevented the loss of the smallest particles and minimized their clumping. Density-gradient ultracentrifugation was designed to remove contaminants and recover the particles without filtration, depositing them directly onto a silicon wafer. This provided uniform distribution of the particles and high contrast against the background, facilitating accurate, automated, morphometric image analysis. The accuracy and precision of the new protocol were assessed by recovering and characterizing particles from wear tests of three types of polyethylene acetabular cups (no crosslinking and 5 Mrads and 7.5 Mrads of gamma irradiation crosslinking). Results The new method demonstrated important differences in the particle size distributions and morphologic parameters among the three types of polyethylene that could not be detected using prior isolation methods. Conclusion The new protocol overcomes a number of limitations, such as loss of nanometer-sized particles and artifactual clumping, among others. Clinical Relevance The analysis of polyethylene wear particles produced in joint simulator wear tests of prosthetic joints is a key tool to identify the wear mechanisms that produce the particles and predict and evaluate their effects on periprosthetic tissues.

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“…For the MoP prostheses, most of the particles are in the µm range with few in the submicron size, which corresponds to the size of bacteria (Margevicius et al 1994, Shanbhag et al 1994, Campbell et al 1995. Polyethylene particles have been shown to elicit a foreign-body, granulomatous response simulating infection, which is non-specific and consists mainly of macrophages and fibroblasts with occasional lymphocytes.…”

Section: Metal-induced Immune Responsesmentioning

confidence: 99%

Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

et al. 2008

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Composition and morphology of wear debris in failed uncemented total hip replacement

Cited by 372 publications

References 26 publications

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

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