Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran

Abstract

The genus Dorema (Apiaceae) is represented in the flora of Iran with seven species of which two, D. ammoniacum D. Don. and D. aucheri Boiss. are endemic. Ripe fruits of D. ammoniacum collected just in the deciduous time were subjected to hydrodistillation to yield the essential oil, which was subsequently analyzed by GC and GC-MS. Twenty-nine compounds were identified and quantified, representing 95.1 % of the total oil. (Z)-Ocimenone (22.3 %) and (E)-ocimenone (18.1 %) were the main components of the oil. In vitro antimicrobial activity of the oil was evaluated against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial assay of the oil by the disc diffusion method and the MIC values indicated that the oil exhibited moderate to high antimicrobial activity, especially against B. subtilis and S. epidermidis with MIC value of 3.75 mg ml-1.

Composition, antioxidant and antimicrobial activity of the essential oils of a full grown tree of Pinus cembra L. from the Calimani mountains (Romania)

“…The EOT also had moderate activity against C. albicans (inhibition zone of 9.66 mm). 26 Unlike EOT, EON showed no antifungal activity. (Table IV).…”

Compounds from Gum Ammoniacum with Acetylcholinesterase Inhibitory Activity

“…Some biological activities such as antibacterial and vasodilatory effects have been reported for this resin [6, 8]. Recently, a low cytotoxic activity was shown for the essential oil from fruits of D. ammoniacum [9]. Additionally, it is listed in the British Herbal Pharmacopoeia as an antispasmodic and expectorant, and it is used occasionally for chronic bronchitis and persistent coughs [10, 11].…”

Anti-inflammatory effect of new kopetdaghins A, C and E fromDorema kopetdaghense

“…Among them, Dorema kopetdaghense Pimenov and D. ammoniacum D. Don are endemic to Iran and have been collected for their resins in Persia since 4000 years ago (Duthie, 1956;Hooper, 1937;Jafari, Chahouki, Tavili, Azarnivand, & Amiri, 2004;). Moreover, members of the Dorema species possess several biological activities (Asnaashari, Dadizadeh, Talebpour, Eskandani, & Nazemiyeh, 2011;Bahraminejad, 2012;Nabavi, Nabavi, & Ebrahimzadeh, 2012;Prashanth Kumar, Chauhan, Padh, & Rajani, 2006;Rajani, Saxena, Ravishankara, Desai, & Padh, 2002;Ram, Balachandar, Vijayananth, & Singh, 2011;Yousefzadi et al, 2011).…”

“…It exhibits remarkable antibacterial and antifungal properties that support its folkloric application in the treatment of a number of infectious diseases (Prashanth Kumar et al, 2006;Rajani et al, 2002). Moreover, the essential oil from its ripe fruits shows in vitro toxicity on human cancer cell lines (Yousefzadi et al, 2011). Extracts and derivatives of D. aitchisonii Korovin ex Pimenov show excellent antihaemolytic and antifungal but weak antioxidant activities (Bahraminejad, 2012;Nabavi et al, 2012).…”