Composition and functions of the extracellular polymer matrix of bacterial biofilms

Журина, М. В.; Ганнесен, А. В.; Zdorovenko, Evelina L.; Плакунов, В. К.

doi:10.1134/s002626171406023x

Cited by 18 publications

(9 citation statements)

References 85 publications

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“…Indeed, when the RT4 strain was grown in the more polar medium (BHI) it was forming biofilm in abundance whereas this activity was reduced in RCM and even more in SLM. Biofilm is a defence mechanism for bacteria and a strategy to resist in unfavorable environments (Zhurina, Gannesen, Zdorovenko, & Plakunov, ), as for the RT4 in BHI. The same was observed, but at the opposite, with the RT6 strain.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

et al. 2019

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Cutibacterium acnes, former Proprionibacterium acnes, is a heterogeneous species including acneic bacteria such as the RT4 strain, and commensal bacteria such as the RT6 strain. These strains have been characterized by metagenomic analysis but their physiology was not investigated until now. Bacteria were grown in different media, brain heart infusion medium (BHI), reinforced clostridial medium (RCM), and in sebum like medium (SLM) specifically designed to reproduce the lipid rich environment of the sebaceous gland. Whereas the RT4 acneic strain showed maximal growth in SLM and lower growth in RCM and BHI, the RT6 non acneic strain was growing preferentially in RCM and marginally in SLM. These differences were correlated with the lipophilic surface of the RT4 strain and to the more polar surface of the RT6 strain. Both strains also showed marked differences in biofilm formation activity which was maximal for the RT4 strain in BHI and for the RT6 strain in SLM. However, cytotoxicity of both strains on HaCaT keratinocytes remained identical and limited. The RT4 acneic strain showed higher inflammatory potential than the RT6 non acneic strain, but the growth medium was without significant influence. Both bacteria were also capable to stimulate β‐defensine 2 secretion by keratinocytes but no influence of the bacterial growth conditions was observed. Comparative proteomics analysis was performed by nano LC‐MS/MS and revealed that whereas the RT4 strain only expressed triacylglycerol lipase, the principal C. acnes virulence factor, when it was grown in SLM, the RT6 strain expressed another virulence factor, the CAMP factor, exclusively when it was grown in BHI and RCM. This study demonstrates the key influence of growth conditions on virulence expression by C. acnesand suggest that acneic and non acneic strains are related to different environmental niches.

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Section: Discussionmentioning

confidence: 99%

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

et al. 2019

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“…Our previous work revealed that the EPS produced by B . licheniformis CGMCC2876 contains an abundant amount of amino sugars (Xiong et al, ), and polysaccharides can also be used as carbon or nitrogen sources(Ferreira et al, ; Zhurina et al, ). Similarly, there are studies reporting that microorganisms secrete γ‐PGA depolymerase to degrade γ‐PGA as a nutrient source when the medium only contains γ ‐PGA (Jeong et al, ; Kunioka and Goto, ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of Bacillus licheniformis reveals a stress response mechanism in the synthesis of extracellular polymeric flocculants

Chen

Shen

et al. 2015

Biotech & Bioengineering

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Some bioflocculants composed of extracellular polymeric substances are produced under peculiar conditions. Bacillus licheniformis CGMCC2876 is a microorganism that secretes both extracellular polysaccharides (EPS) and poly-gamma-glutamic acid (γ-PGA) under stress conditions. In this work, SWATH acquisition LC-MS/MS method was adopted for differential proteomic analysis of B. licheniformis, aiming at determining the bacterial stress mechanism. Compared with LB culture, 190 differentially expressed proteins were identified in B. licheniformis CGMCC2876 cultivated in EPS culture, including 117 up-regulated and 73 down-regulated proteins. In γ-PGA culture, 151 differentially expressed proteins, 89 up-regulated and 62 down-regulated, were found in the cells. Up-regulated proteins involved in amino acid biosynthesis were found to account for 43% and 41% of the proteomes in EPS and γ-PGA cultivated cells, respectively. Additionally, a series of proteins associated with amino acid degradation were found to be repressed under EPS and γ-PGA culture conditions. Transcriptional profiling via the qPCR detection of selected genes verified the proteomic analysis. Analysis of free amino acids in the bacterial cells further suggested the presence of amino acid starvation conditions. EPS or γ-PGA was synthesized to alleviate the effect of amino acid limitation in B. licheniformis. This study identified a stress response mechanism in the synthesis of macromolecules in B. licheniformis, providing potential culture strategies to improve the production of two promising bioflocculants.

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“…Like arthropods, bacteria use amyloids as a structural scaffold for biofilm formation. Biofilms are dense communities of bacteria in which cells associate with surfaces and each other using a self‐produced extracellular matrix (EM) composed of proteins, polysaccharides, and genetic material (Zhurina et al, ). Proteins in the biofilm EM may take on a variety of forms, but amyloid structures are common, and recent research suggests that many multi‐drug resistant (MDR) bacterial strains utilize amyloid fibrils to facilitate bacterial aggregation and biofilm formation as an underlying mechanism of virulence (DePas and Chapman, ; Romero and Kolter, ; Syed and Boles, ; Zhou et al, ).…”

Section: Functional Amyloidsmentioning

confidence: 99%

Structural and functional diversity among amyloid proteins: Agents of disease, building blocks of biology, and implications for molecular engineering

Bleem

Daggett

2016

Biotech & Bioengineering

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Amyloids have long been associated with protein dysfunction and neurodegenerative diseases, but recent research has demonstrated that some organisms utilize the unique properties of the amyloid fold to create functional structures with important roles in biological processes. Additionally, new engineering approaches have taken advantage of amyloid structures for implementation in a wide variety of materials and devices. In this review, the role of amyloid in human disease is discussed and compared to the functional amyloids, which serve a largely structural purpose. We then consider the use of amyloid constructs in engineering applications, including their utility as building blocks for synthetic biology and molecular engineering. Biotechnol. Bioeng. 2017;114: 7-20. © 2016 Wiley Periodicals, Inc.

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Composition and functions of the extracellular polymer matrix of bacterial biofilms

Cited by 18 publications

References 85 publications

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

Proteomic profiling of Bacillus licheniformis reveals a stress response mechanism in the synthesis of extracellular polymeric flocculants

Structural and functional diversity among amyloid proteins: Agents of disease, building blocks of biology, and implications for molecular engineering

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