Composition and functional group characterization of extracellular polymeric substances (EPS) in activated sludge: the impacts of polymerization degree of proteinaceous substrates

Organic loading rate shock impact on extracellular polymeric substances and physicochemical characteristics of nitrifying sludge treating high-strength ammonia wastewater under unsteady-state conditions

“…21 Some researchers suggested that humic compounds in EPS were mainly adsorbed from the wastewater and hydrolysis process. 40 Previous studies demonstrated that these humic acid-like substances and fulvic acid-like substances were likely to deteriorate to sludge occulation, which reasonably interpreted the deterioration of occulability under high OLRs. 12,21,41 The increase in humic acidlike substances in the operation of NS might be used as an important indicator of oc structure deterioration since it could affect the bioocculation.…”

Density-Based Separation of Microbial Functional Groups in Activated Sludge

“…While we used slow-speed centrifuge for biomass separation with the intention to maintain original floc structure, it is possible that this procedure had slightly modified floc structure and thus contributed to the overall enrichment effects. Additionally, EPS physicochemistry, particularly polymerization degree of proteinaceous substrates, has large impact on EPS functionality [53,54], which in turn influences floc stability and AS flocculation [7][8][9]. Future research should address the impact of EPS on density-based enrichment of microbial groups.…”

An improved ASM-GDA approach to evaluate the production kinetics of loosely bound and tightly bound extracellular polymeric substances in biological phosphorus removal process

“…4 However, recent studies reveal that microbial cells embedded in their surrounding matrix of EPS collectively form a semi-solid structure and exhibit an important role in biological wastewater treatment processes. [5][6][7] As a complex high-molecularweight mixture of polymers, the accepted consensus for EPS classication in the literature contains the tightly bound EPS (TB-EPS) forming the inner layer, the loosely bound EPS (LB-EPS) diffusing in the outer layer, and soluble EPS originating from EPS hydrolysis. 8,9 As an inevitable transportation pathway of P between bulk solution and PAOs cell, both TB-EPS and LB-EPS are regarded as the P reservoirs, and hence pose signicantly inuences on the uptake and release of P in BPR processes.…”