Composition and functional diversity of fecal bacterial community of wild boar, commercial pig and domestic native pig as revealed by 16S rRNA gene sequencing

Huang, Jinqing; Zhang, Wenjuan; Fan, Rong; Liu, Zhanggen; Huang, Tao; Li, Junyi; Du, Tonghao; Xiong, Tao

doi:10.1007/s00203-019-01787-w

Cited by 25 publications

(22 citation statements)

References 60 publications

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“…Thus, wild pigs might benefit from the high abundance of Lactobacillus in the gut. However, previous studies usually observed a higher abundance of Lactobacillus in the gut of commercial and domestic native pigs than wild pigs ( 12 , 24 ), which is inconsistent with our results, partly due to the differential environment or geography ( 25 , 26 ).…”

Section: Discussioncontrasting

confidence: 99%

“…It has been previously reported that a strong distinction existed in the bacterial diversity of wild pigs and domestic pigs. What is more, predictions of metagenome function showed more bacterial genes related to the immune system and environmental adaptation in the feces of wild pigs than that in domestic pigs ( 12 ). To investigate the probiotics in wild pigs, Li and colleagues assessed the probiotic characteristics and safety properties of LAB strains isolated from the gut, including L. mucosae , L. salivarius , Enterococcus hirae , Enterococcus durans , and Enterococcus faecium ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Lactic Acid Bacteria Mixture Isolated From Wild Pig Alleviated the Gut Inflammation of Mice Challenged by Escherichia coli

Zhong

Deng

et al. 2022

Front. Immunol.

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Wild pigs usually showed high tolerance and resistance to several diseases in the wild environment, suggesting that the gut bacteria of wild pigs could be a good source for discovering potential probiotic strains. In our study, wild pig feces were sequenced and showed a higher relative abundance of the genus Lactobacillus (43.61% vs. 2.01%) than that in the domestic pig. A total of 11 lactic acid bacteria (LAB) strains including two L. rhamnosus, six L. mucosae, one L. fermentum, one L. delbrueckii, and one Enterococcus faecalis species were isolated. To investigate the synergistic effects of mixed probiotics strains, the mixture of 11 LAB strains from an intestinal ecology system was orally administrated in mice for 3 weeks, then the mice were challenged with Escherichia coli ATCC 25922 (2 × 109 CFU) and euthanized after challenge. Mice administrated with LAB strains showed higher (p < 0.05) LAB counts in feces and ileum. Moreover, alterations of specific bacterial genera occurred, including the higher (p < 0.05) relative abundance of Butyricicoccus and Clostridium IV and the lower (p < 0.05) abundance of Enterorhabdus in mice fed with mixed LAB strains. Mice challenged with Escherichia coli showed vacuolization of the liver, lower GSH in serum, and lower villus to the crypt proportion and Claudin-3 level in the gut. In contrast, administration of mixed LAB strains attenuated inflammation of the liver and gut, especially the lowered IL-6 and IL-1β levels (p < 0.05) in the gut. Our study highlighted the importance of gut bacterial diversity and the immunomodulation effects of LAB strains mixture from wild pig in gut health.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lactic Acid Bacteria Mixture Isolated From Wild Pig Alleviated the Gut Inflammation of Mice Challenged by Escherichia coli

Zhong

Deng

et al. 2022

Front. Immunol.

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“…A Tax4Fun analysis was performed to investigate microbiota function (Huang et al. 2020 ). The method provides an effective link between the OTU species classification annotated by the SILVA database and the prokaryotic metabolism function in the KEGG database.…”

Section: Resultsmentioning

confidence: 99%

Antidepressant effects of total iridoids of Valeriana jatamansi via the intestinal flora-blood–brain barrier pathway

Zhang

Wang

Huang

et al. 2021

Pharmaceutical Biology

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Context: Valeriana jatamansi Jones [syn. V. wallichii DC, (Valerianaceae)] (VJJ) is used to treat depression. Objective: To explore the effects of total iridoids of VJJ extract (TIV) on chronic unpredictable mild stress (CUMS) in mice. Materials and methods: VJJ roots and rhizomes were extracted with 70% ethanol. CUMS rats were treated daily with fluoxetine (2.6 mg/kg, i.g.) or TIV (5.7, 11.4, and 22.8 mg/kg, i.g.) for 14 days. Male Kun Ming mice on normal chow and 0.5% CMC-Na solution were used as a control. Behavioural tests included the tail suspension (TST) and sucrose preference tests (SPT). Evans blue staining was used to evaluate blood-brain barrier (BBB) permeability. Western blotting was used to measure zonula occludens-1 (ZO-1) and occludin expression. 16S rRNA sequencing was used to analyse intestinal flora abundance. Tax4Fun was used to predict KEGG metabolic pathways. Results: TIV treatment reduced TST time (117.35 ± 8.23 or 108.95 ± 6.76 vs. 144.45 ± 10.30 s), increased SPT (55.83 ± 7.24 or 53.12 ± 13.85 vs. 38.98 ± 5.43%), increased the abundance of phylum Firmicutes (86.99 ± 0.03 vs. 60.88 ± 0.19%) and genus Lactobacillus (75.20 ± 0.19 vs. 62.10 ± 0.13%), reduced the abundance of phylum Bacteroidetes (6.69 ± 0.06 or 11.50 ± 0.09 vs. 25.07 ± 0.20%). TIV increased carbohydrate metabolism (14.50 ± 3.00 Â 10 À3 or 14.60 ± 2.00 Â 10 À3 or 14.90 ± 2.00 Â 10 À3 vs.13.80 ± 4.00 Â 10 À3 %), replication and repair functions (5.60 ± 1.00 Â 10 À3 or 5.60 ± 1.00 Â 10 À3 vs. 5.10 ± 4.00 Â 10 À3 %), reduced the frequency of infectious disease (1.60 ± 2.00 Â 10 À4 or 1.90 ± 5.00 Â 10 À4 or 1.80 ± 3.00 Â 10 À4 vs. 2.20 ± 7.00 Â 10 À3 %), BBB permeability (0.77 ± 0.30 vs. 1.81 ± 0.33 lg/g), and up-regulated the expression of ZO-1 (1.42-fold, 1.60-fold, 1.71-fold) and occludin (1.79-fold, 2.20-fold). Conclusions: TIV may modulate the intestinal flora, thereby inducing the expression of ZO-1 and occludin, protecting the BBB and exerting an antidepressant effect.

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“…According to outcomes of the functional prediction, the situation of one or more samples at a higher level of function can be known, and the functional structure of samples at a higher level can be observed [33]. In this study, the histogram of functional structure distribution based on COG is shown in Fig.…”

Section: Changes Of Community Structurementioning

confidence: 96%

<i>In-situ</i> Bioremediation of Multiple Heavy Metals Contaminated Farmland Soil by Sulfate-Reducing Bacteria

Lv¹,

Zhu²,

Zhang³

et al. 2022

Pol. J. Environ. Stud.

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Heavy metal pollution of farmland soil deteriorates the cultivated land quality, and adversely affects the growth, yield, and quality of crops. Besides, it hampers food safety, and poses a tremendous threat to human health. In this study, an in-situ bioremediation process was investigated in maize planting areas by spraying sulfate-reducing bacteria solution based on previous studies. The altered basic physical and chemical properties of heavy metals in soil were studied using the Tessier sequential extraction technique to investigate the efficiency of the bioremediation process. The environmental effects of the bioremediation process were also explored by investigating the crop characteristics. The analysis of microbial species composition was used to explore the influence of exogenous microbes on microbial composition in farmland soil. The outcomes of remediation experiments showed that the sulfate-reducing bacteria could stabilize heavy metals in soil, inhibit the uptake of heavy metals by plants, improve the quality of maize products and promote the growth of maize plants. In this study, we investigated the in-situ bioremediation of multiple heavy metals contaminated farmland soil using sulfate-reducing bacteria. The outcomes of the study revealed the unique advantages of bioremediation technology in practical application of heavy metals contaminated farmland soil.

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Composition and functional diversity of fecal bacterial community of wild boar, commercial pig and domestic native pig as revealed by 16S rRNA gene sequencing

Cited by 25 publications

References 60 publications

Lactic Acid Bacteria Mixture Isolated From Wild Pig Alleviated the Gut Inflammation of Mice Challenged by Escherichia coli

Lactic Acid Bacteria Mixture Isolated From Wild Pig Alleviated the Gut Inflammation of Mice Challenged by Escherichia coli

Antidepressant effects of total iridoids of Valeriana jatamansi via the intestinal flora-blood–brain barrier pathway

<i>In-situ</i> Bioremediation of Multiple Heavy Metals Contaminated Farmland Soil by Sulfate-Reducing Bacteria

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