Composition and diversity of the bacterial community in snow leopard (Uncia uncia) distal gut

Zhang, Honghai; Liu, Guangshuai; Lei, Chen; Sha, Weilai

doi:10.1007/s13213-014-0909-9

Cited by 8 publications

(15 citation statements)

References 25 publications

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“…Within the phylum Firmicutes, Zhang et al (2015) found that Lachnospiraceae was the most diverse family in the feces of snow leopards, which is consistent with a previous report in wolves (Zhang & Chen 2010). In our results, however, the most diverse family was Clostridiaceae 1 (19.5% in North Chinese leopard, 17.2% in Amur leopard) within the order Clostridiales, and Lachnospiraceae constituted a relatively small proportion in our sample set compared to snow leopards and wolves.…”

Section: Discussionsupporting

confidence: 92%

“…Previous researches have reported that Firmicutes is the most dominant phylum in feces of animals (Garcia-Mazcorro et al 2012;Guan et al 2017;Ritchie et al 2010) and humans (Arumugam et al 2011). Same tendency was also found in feline species in the wild such as leopard cats (Prionailurus bengalensis) (An et al 2017) and snow leopards (Panthera uncia) (Zhang et al 2015). Some studies reported that the body fat storage influences the gut bacterial composition in mice (Ley et al 2005) and humans (Ley et al 2006).…”

Section: Discussionmentioning

confidence: 88%

“…The phylum Actinobacteria also contributed to the gut microbiota of the leopards and its proportion was not significantly different between the two subspecies (p = 0.45). It was identified to be the most predominant phylum in snow leopards (Zhang et al 2015). In contrast, Wu et al (2017) reported that Actinobacteria constituted 0.53% of all gut bacteria in wolves (Canis lupus).…”

Section: Discussionmentioning

confidence: 96%

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Peer Review #2 of "Comparison of the fecal microbiota of two free-ranging Chinese subspecies of the leopard (Panthera pardus) using high-throughput sequencing (v0.1)"

2019

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The analysis of gut microbiota using fecal samples provides a non-invasive approach to understand the complex interactions between host species and their intestinal bacterial community. However, information on gut microbiota for wild endangered carnivores is scarce. The goal of this study was to describe the gut microbiota of two leopard subspecies, the Amur leopard (Panthera pardus orientalis) and North Chinese leopard (Panthera pardus japonensis). Fecal samples from the Amur leopard (n=8) and North Chinese leopard (n=13) were collected in Northeast China Tiger and Leopard National Park and Shanxi Tieqiaoshan Provincial Nature Reserve, respectively. The gut microbiota of leopards was analyzed via high-throughput sequencing of the V3-V4 region of bacterial 16S rRNA gene using the Life Ion S5TM XL platform. A total of 1,413,825 clean reads representing 4,203 operational taxonomical units (OTUs) were detected. For Amur leopard samples, Firmicutes (78.4%) was the dominant phylum, followed by Proteobacteria (9.6%) and Actinobacteria (7.6%). And for North Chinese leopard, Firmicutes (68.6%), Actinobacteria (11.6%) and Proteobacteria (6.2%) were the most predominant phyla. Clostridiales was the most diverse bacterial order with 37.9% for Amur leopard and 45.7% for North Chinese leopard. Based on the beta-diversity analysis, no significant difference was found in the bacterial community composition between the Amur leopard and North Chinese leopard samples. The current study provides the initial data about the composition and structure of the gut microbiota for wild Amur leopards and North Chinese leopards, and has laid the foundation for further investigations of the health, dietary preferences and physiological regulation of leopards.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 96%

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Peer Review #2 of "Comparison of the fecal microbiota of two free-ranging Chinese subspecies of the leopard (Panthera pardus) using high-throughput sequencing (v0.1)"

2019

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“…Firmicutes, Bacteroides, Actinobacteria and Proteobacteria were the main microbes of the captive and wild leopards, consistent with the results of studies on other carnivores, such as snow leopard [19,20], wolves [21], Amur tiger [22], Amur leopard and cheetahs [25,26] [19][20][21][22][23][24][25][26]. Fecal samples of healthy domestic cats (Felis catus) [27]and snow leopard (Panthera uncia) [19,20,28] featured similar phylum compositions with slightly different proportions. In our study, the relative abundance of Fusobacteria of captive and wild leopards was signi cantly different (p=0.036), but other phyla showed no signi cant differences.…”

Section: Gut Microbiota Of North China Leopard and Comparison With Thsupporting

confidence: 88%

“…8%) and wild North China leopard (23.4%), and there was no signi cant different between the two groups (p = 0.473). In snow leopard [19,20], Amur tiger [29] and North China leopard (Panthera tigris altaica) [24], Actinobacteria was the predominant phylum. In contrast, Wu reported that Actinobacteria constituted 0.53% in wolves [34].…”

Section: Gut Microbiota Of North China Leopard and Comparison With Thmentioning

confidence: 99%

Gut microbiota and fecal metabolites in captive and wild North China leopard (Panthera pardus japonensis) by comparsion using 16s rRNA gene sequencing and LC/MS-based metabolomics

Hua

Cao

Wang

et al. 2020

Preprint

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Background: Gut microbes significantly contribute to nutrient digestion and absorption, intestinal health and immunity, and are essential for the survival and environmental adaptation of wild animals. However, there are few studies on the gut microbiota of captive and wild North China leopard (Panthera pardus japonensis). Results: A total of 10 mainly bacterial phyla were identified in the fecal microbiota of North China leopard, Lachnoclostridium (p = 0.003), Peptoclostridium (p = 0.005), Bacteroides (p =0.008), Fusobacterium (p = 0.017) and Collinsella (p = 0.019) were significantly higher than those of wild North China leopard. Distinct differences in the fecal metabolic phenotypes of captive and wild North China leopard were found, such as content of l-methionine, n-acetyl-l-tyrosine, pentadecanoic acid and oleic acid. Differentially abundant gut microbes were associated with fecal metabolites, especially the bacteria in Firmicutes and Bacteroidetes, involved in the metabolism of N-acetyl-L-alanine and D-quinovose.Conclusion: This study reports for the first time the differences in gut microbiota abundance between captive and wild North China leopard, as well as significant differences in fecal metabolic phenotypes between two groups.

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Snow leopard status and conservation in China

Shi,

Xiao,

Atzeni

et al. 2024

Snow Leopards

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Composition and diversity of the bacterial community in snow leopard (Uncia uncia) distal gut

Cited by 8 publications

References 25 publications

Peer Review #2 of "Comparison of the fecal microbiota of two free-ranging Chinese subspecies of the leopard (Panthera pardus) using high-throughput sequencing (v0.1)"

Peer Review #2 of "Comparison of the fecal microbiota of two free-ranging Chinese subspecies of the leopard (Panthera pardus) using high-throughput sequencing (v0.1)"

Gut microbiota and fecal metabolites in captive and wild North China leopard (Panthera pardus japonensis) by comparsion using 16s rRNA gene sequencing and LC/MS-based metabolomics

Snow leopard status and conservation in China

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