Composition and Biosynthesis of Scent Compounds from Sterile Flowers of an Ornamental Plant Clematis florida cv. ‘Kaiser’

Jiang, Yifan; Qian, Renjuan; Zhang, Wanbo; Guo, Wei; Ma, Xiaojuan; Zheng, Jian; Köllner, Tobias G.; Chen, Feng

doi:10.3390/molecules25071711

Cited by 12 publications

(5 citation statements)

References 36 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are consistent with the general observation that terpenes are major constituents of floral scents in many plant species [7,42,[51][52][53]; this may also be the function of the floral terpenes produced by P. ruthii [3,7]. α-Pinene is the predominant monoterpene detected from P. ruthii flowers and probably acts as an important chemical cue to attract pollinators, and similar to observations in Eucalyptus polybractea [54], or the moths Helicoverpa armigera reacting to this terpene [55].…”

Section: Plos Onesupporting

confidence: 91%

Chemical profile and analysis of biosynthetic pathways and genes of volatile terpenes in Pityopsis ruthii, a rare and endangered flowering plant

et al. 2023

Self Cite

View full text Add to dashboard Cite

It is critical to gather biological information about rare and endangered plants to incorporate into conservation efforts. The secondary metabolism of Pityopsis ruthii, an endangered flowering plant that only occurs along limited sections of two rivers (Ocoee and Hiwassee) in Tennessee, USA was studied. Our long-term goal is to understand the mechanisms behind P. ruthii’s adaptation to restricted areas in Tennessee. Here, we profiled the secondary metabolites, specifically in flowers, with a focus on terpenes, aiming to uncover the genomic and molecular basis of terpene biosynthesis in P. ruthii flowers using transcriptomic and biochemical approaches. By comparative profiling of the nonpolar portion of metabolites from various tissues, P. ruthii flowers were rich in terpenes, which included 4 monoterpenes and 10 sesquiterpenes. These terpenes were emitted from flowers as volatiles with monoterpenes and sesquiterpenes accounting for almost 68% and 32% of total emission of terpenes, respectively. These findings suggested that floral terpenes play important roles for the biology and adaptation of P. ruthii to its limited range. To investigate the biosynthesis of floral terpenes, transcriptome data for flowers were produced and analyzed. Genes involved in the terpene biosynthetic pathway were identified and their relative expressions determined. Using this approach, 67 putative terpene synthase (TPS) contigs were detected. TPSs in general are critical for terpene biosynthesis. Seven full-length TPS genes encoding putative monoterpene and sesquiterpene synthases were cloned and functionally characterized. Three catalyzed the biosynthesis of sesquiterpenes and four catalyzed the biosynthesis of monoterpenes. In conclusion, P. ruthii plants employ multiple TPS genes for the biosynthesis of a mixture of floral monoterpenes and sesquiterpenes, which probably play roles in chemical defense and attracting insect pollinators alike.

show abstract

Section: Plos Onesupporting

confidence: 91%

Chemical profile and analysis of biosynthetic pathways and genes of volatile terpenes in Pityopsis ruthii, a rare and endangered flowering plant

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…In flowering plants, terpenoids are widely present in the volatile metabolites of plants. For example, during the bloom period, over 20 volatile terpenes are released from Clematis florida [37]. The wild species of the Freesia genus displays a variety of terpenes [38].…”

Section: Discussionmentioning

confidence: 99%

Identification of Volatile Compounds and Terpene Synthase (TPS) Genes Reveals ZcTPS02 Involved in β-Ocimene Biosynthesis in Zephyranthes candida

Wei,

Xu,

et al. 2024

Genes

Self Cite

View full text Add to dashboard Cite

Zephyranthes candida is a frequently cultivated ornamental plant containing several secondary metabolites, including alkaloids, flavonoids, and volatile organic compounds (VOCs). However, extensive research has been conducted only on non-VOCs found in the plant, whereas the production of VOCs and the molecular mechanisms underlying the biosynthesis of terpenes remain poorly understood. In this study, 17 volatile compounds were identified from Z. candida flowers using gas chromatography–mass spectrometry (GC-MS), with 16 of them being terpenoids. Transcriptome sequencing resulted in the identification of 17 terpene synthase (TPS) genes; two TPS genes, ZcTPS01 and ZcTPS02, had high expression levels. Biochemical characterization of two enzymes encoded by both genes revealed that ZcTPS02 can catalyze geranyl diphosphate (GPP) into diverse products, among which is β-ocimene, which is the second most abundant compound found in Z. candida flowers. These results suggest that ZcTPS02 plays a vital role in β-ocimene biosynthesis, providing valuable insights into terpene biosynthesis pathways in Z. candida. Furthermore, the expression of ZcTPS02 was upregulated after 2 h of methyl jasmonate (MeJA) treatment and downregulated after 4 h of the same treatment.

show abstract

“…The proportion of terpenes is greatly reduced in open flowers. This phenomenon occurs in the flowers of most fragrant plants, including Plumeria rubra flowers [147], lemon basil (O. citriodorum Vis) [148], roses [55], J. auriculatum [32], J. grandiflorum flowers [34], styrax flowers [56], M. tenuifolia [105], C. sativa [72] and C. goeringii [79]. Moreover, the circadian rhythm strongly influences the release of FVTs including (Z)-β-ocimene and (+/−)-linalool from lilium 'Siberia' [104], myrcene and (E)-β-ocimene from snapdragon flowers, 1,8-cineole from N. suaveolens [116] and linalool and its enantiomers from Jasminum spp.…”

Section: Spatio-temporal Regulationmentioning

confidence: 99%

An Update on the Function, Biosynthesis and Regulation of Floral Volatile Terpenoids

Qiao

Shi

et al. 2021

Horticulturae

View full text Add to dashboard Cite

Floral volatile terpenoids (FVTs) belong to a group of volatile organic compounds (VOC) that play important roles in attracting pollinators, defending against pathogens and parasites and serving as signals associated with biotic and abiotic stress responses. Although research on FVTs has been increasing, a systematic generalization is lacking. Among flowering plants used mainly for ornamental purposes, a systematic study on the production of FVTs in flowers with characteristic aromas is still limited. This paper reviews the biological functions and biosynthesis of FVTs, which may contribute a foundational aspect for future research. We highlight regulatory mechanisms that control the production of FVTs in ornamental flowers and the intersection of biosynthetic pathways that produce flower fragrance and color. Additionally, we summarize the opportunities and challenges facing FVT research in the whole genome and -omics eras and the possible research directions that will provide a foundation for further innovation and utilization of flowering ornamental plants and their germplasm resources.

show abstract

Composition and Biosynthesis of Scent Compounds from Sterile Flowers of an Ornamental Plant Clematis florida cv. ‘Kaiser’

Cited by 12 publications

References 36 publications

Chemical profile and analysis of biosynthetic pathways and genes of volatile terpenes in Pityopsis ruthii, a rare and endangered flowering plant

Chemical profile and analysis of biosynthetic pathways and genes of volatile terpenes in Pityopsis ruthii, a rare and endangered flowering plant

Identification of Volatile Compounds and Terpene Synthase (TPS) Genes Reveals ZcTPS02 Involved in β-Ocimene Biosynthesis in Zephyranthes candida

An Update on the Function, Biosynthesis and Regulation of Floral Volatile Terpenoids

Contact Info

Product

Resources

About