Composition and Antimicrobial Activity of the Essential Oil from Leaves of Curcuma longa L. Kasur Variety

Abstract

The essential oil from the leaves of Curcuma longa L. Kasur variety grown in Pakistan was extracted by hydro-distillation. Chemical constituents of the essential oil were identified by gas chromatography/mass spectrometry. The chromatographic analysis of oil showed 25 constituents, out of which nine chemical constituents were identified. The eucalyptol (10.27%) was the major component of the essential oil. α-pinene (1.50%), β-phellandrene (2.49%), β-pinene (3.57%), limonene (2.73%), 1,3,8-p-menthatriene (1.76%), ascaridole epoxide (1.452%), 2-methylisoborneol (2.92%), 5-isopropyl-6-methyl-hepta-3, dien-2-ol (2.07%) were also present in considerable quantity. The antimicrobial properties of leaves of Curcuma longa were tested by disc diffusion method against various human pathogens, including eight fungal and five bacterial strains. Essential oil showed maximum resistance against Fusarium miniformes MAY 3629 followed by Bacillus subtilis ATCC 6633 whereas; it exhibited least resistance against Fusarium oxysporium ATCC 48122. The results of the antimicrobial assay revealed that essential oil showed significant inhibitory activity against the tested organisms.

Inhibition of the mycelial growth and germination of Stromatinia cepivora by plant extracts and essential oils

“…Some fungitoxic substances were already identified in mentrasto Esper et al, 2014), rue (Domingues et al, 2009;Azizi & Karouei, 2012), ginger (Sharma & Tiwari, 2013), false saffron (Ferreira et al, 2013;Parveen et al, 2013), citronella (Simic et al, 2008), rosemary (Ozcan & Chalchat, 2008), neem (Coventry & Allan, 2001) and eucalyptus citriodora (Aguiar et al, 2014). It is likely that fungicidal substances of wide spectrum present in the extracts and essential oils of these plants are involved in the inhibition of S. cepivora, such as precoceno in mentrasto , citronellal in citronella and eucalyptus citriodora (Simic et al, 2008;Tomazi et al, 2014), 1.8-cineole (eucalyptol) in ginger and rosemary (Ozcan & Chalchat, 2008;Sharma & Tiwari, 2013) and azadirachtin in neem (Coventry & Allan, 2001).…”

Antimicrobial effect of essential oils of some Fijian medicinal plant leaves on pathogenic bacteria

“…In vitro studies clearly showed that the studied EO inhibited pathogenic growth but their effectiveness varied ( Table 2). The antimicrobial activities of many EO have been reviewed and classified by previous researchers as strong, medium and weak or resistant, intermediate and sensitive (Parveen et al, 2013;Zaika, 1988). Zaika (1988) also proposed that Gram-positive bacteria are more resistant than Gram-negative bacteria to the antibacterial properties of plant volatile oils which is in contrast to the hypothesis proposed by other researchers where the susceptibility of bacteria to plant volatile oils appears to have little influence on growth inhibition (Deans and Ritchie, 1987;Deans et al, 1995).…”

A phytochemical of modern medicinal perspective

“…The GC-MS analysis of oil showed the presence of 25 constituents and out of them eucalyptol was found to be the chief constituent. Ascaridole epoxide and some other phytoconstituents were also present in considerable amount [35]. Hydrodistillation method was used for the separation of the essential oils of the leaves of Artemisia vulgaris, Artemisia indica and Artemisia dubia and GC-MS method was used for their analysis.…”