Composite vascular grafts with high cell infiltration by co-electrospinning

Tan, Zhikai; Wang, Hongjie; Gao, Xiangkai; Liu, Tong; Tan, Yongjun

doi:10.1016/j.msec.2016.05.067

Cited by 60 publications

(36 citation statements)

References 39 publications

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“…Figure a–h shows different porous 2D and 3D PLGA platforms fabricated using the E‐jet printing system we describe here. We compared our fibers with those produced by electrospinning, as this technique also allows producing very fine nanofibers . However, it is not possible to finely control the structure of the printed fibers using electrospinning, as shown in Figure.…”

Section: Resultsmentioning

confidence: 99%

“…Many different techniques have been developed in the recent years to build cell culture platforms and scaffolds for tissue engineering, including selective laser sintering, stereo lithography, fused filament fabrication, microfabrication, phase separation, electrospinning and 3D printing . Electro‐hydrodynamic jet (E‐jet) printing is a new high‐resolution printing method whereby the geometric feature of the built scaffolds can be precisely controlled using a broad range of materials .…”

Section: Introductionmentioning

confidence: 99%

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Control of cell growth on 3D‐printed cell culture platforms for tissue engineering

Tan

Liu

Zhong

et al. 2017

J Biomedical Materials Res

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Biocompatible tissue growth has excellent prospects for tissue engineering. These tissues are built over scaffolds, which can influence aspects such as cell adhesion, proliferation rate, morphology, and differentiation. However, the ideal 3D biological structure has not been developed yet. Here, we applied the electro-hydrodynamic jet (E-jet) 3D printing technology using poly-(lactic-co-glycolic acid, PLGA) solution to print varied culture platforms for engineered tissue structures. The effects of different parameters (electrical voltage, plotting speed, and needle sizes) on the outcome were investigated. We compared the biological compatibility of the 3D printed culture platforms with that of random fibers. Finally, we used the 3D-printed PLGA platforms to culture fibroblasts, the main cellular components of loose connective tissue. The results show that the E-jet printed platforms could guide and improve cell growth. These highly aligned fibers were able to support cellular alignment and proliferation. Cell angle was consistent with the direction of the fibers, and cells cultured on these fibers showed a much faster migration, potentially enhancing wound healing performance. Thus, the potential of this technology for 3D biological printing is large. This process can be used to grow biological scaffolds for the engineering of tissues. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3281-3292, 2017.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Control of cell growth on 3D‐printed cell culture platforms for tissue engineering

Tan

Liu

Zhong

et al. 2017

J Biomedical Materials Res

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“…Co-electrospinning has been widely used to fabricate vascular grafts due to its unique capacity for integrating the advantages of different biomaterials into a graft 16, 17 . Figure 1A is the schematic diagram for preparing hybrid grafts containing PCL and PDS fibers by co-electrospinning.…”

Section: Resultsmentioning

confidence: 99%

Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers

Pan

Zhou

Wei

et al. 2017

Sci Rep

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Electrospun polycaprolactone (PCL) vascular grafts showed good mechanical properties and patency. However, the slow degradation of PCL limited vascular regeneration in the graft. Polydioxanone (PDS) is a biodegradable polymer with high mechanical strength and moderate degradation rate in vivo. In this study, a small-diameter hybrid vascular graft was prepared by co-electrospinning PCL and PDS fibers. The incorporation of PDS improves mechanical properties, hydrophilicity of the hybrid grafts compared to PCL grafts. The in vitro/vivo degradation assay showed that PDS fibers completely degraded within 12 weeks, which resulted in the increased pore size of PCL/PDS grafts. The healing characteristics of the hybrid grafts were evaluated by implantation in rat abdominal aorta replacement model for 1 and 3 months. Color Doppler ultrasound demonstrated PCL/PDS grafts had good patency, and did not show aneurysmal dilatation. Immunofluorescence staining showed the coverage of endothelial cells (ECs) was significantly enhanced in PCL/PDS grafts due to the improved surface hydrophilicity. The degradation of PDS fibers provided extra space, which facilitated vascular smooth muscle regeneration within PCL/PDS grafts. These results suggest that the hybrid PCL/PDS graft may be a promising candidate for the small-diameter vascular grafts.

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“…The microfibres used in this study were fabricated by electrospinning. Electrospinning is a controlled and fast fibre fabrication method, which allows the spinning of highly aligned or random oriented nano and microfibres, which have seen different applications in tissue engineering [37][38][39] . A range of fibre diameters (1, 5 and 8 µm) were extensively studied by Daud et al, where NG108-15 neuronal cells formed the longest neurites in co-culture experiments together with primary Schwann cells when grown on 5 µm fibres [18] .…”

Section: Discussionmentioning

confidence: 99%

Pre-clinical evaluation of advanced nerve guideconduits using a novel 3D in vitro testing model

Behbehani¹,

Glen²,

Taylor³

et al. 2024

IJB

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Autografts are the current gold standard for large peripheral nerve defects in clinics despite the frequently occurring side effects like donor site morbidity. Hollow nerve guidance conduits (NGC) are proposed alternatives to autografts, but failed to bridge gaps exceeding 3 cm in humans. Internal NGC guidance cues like microfibres are believed to enhance hollow NGCs by giving additional physical support for directed regeneration of Schwann cells and axons. In this study, we report a new 3D in vitro model that allows the evaluation of different intraluminal fibre scaffolds inside a complete NGC. The performance of electrospun polycaprolactone (PCL) microfibres inside 5 mm long polyethylene glycol (PEG) conduits were investigated in neuronal cell and dorsal root ganglion (DRG) cultures in vitro. Z-stack confocal microscopy revealed the aligned orientation of neuronal cells along the fibres throughout the whole NGC length and depth. The number of living cells in the centre of the scaffold was not significantly different to the tissue culture plastic (TCP) control. For ex vivo analysis, DRGs were placed on top of fibre-filled NGCs to simulate the proximal nerve stump. In 21 days of culture, Schwann cells and axons infiltrated the conduits along the microfibres with 2.2 ± 0.37 mm and 2.1 ± 0.33 mm, respectively. We conclude that this in vitro model can help define internal NGC scaffolds in the future by comparing different fibre materials, composites and dimensions in one setup prior to animal testing. Keywords: 3D model; intraluminal scaffold; peripheral nerve; regenerative medicine; microfibres

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Composite vascular grafts with high cell infiltration by co-electrospinning

Cited by 60 publications

References 39 publications

Control of cell growth on 3D‐printed cell culture platforms for tissue engineering

Control of cell growth on 3D‐printed cell culture platforms for tissue engineering

Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers

Pre-clinical evaluation of advanced nerve guideconduits using a novel 3D in vitro testing model

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