Composite of Cell Sheet Fragments and Fibrin Gel for Cartilage Engineering

Zhou, Libin; Zhou, Yang; Piao, Zhengguo; Wu, Wei; Zou, Rui; Ouyang, Kexiong; Zhang, Junwei; Luo, Hao; Chen, Hao; Ge, Linhu

doi:10.1166/jbt.2018.1920

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“…In our previous study, we used chondrocyte cell sheet fragments (chondrocyte-microtissues) harvested from chondrocyte cell sheets to load in fibrin gel for cartilage regeneration purpose. 22 However, the potential of chondrocyte-microtissues laden fibrin gel auricle scaffold for microtia reconstruction has not investigated yet. Homogeneous distribution of microtissues in the fibrin gel auricular scaffold is another challenge due to the viscous nature of microtissues and auricle anatomy complexity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold for microtia reconstruction

et al. 2020

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Fibrin gel-based scaffolds have promising potential for microtia reconstruction. Autologous chondrocytes and chondrocyte cell sheets are frequently used seed cell sources for cartilage tissue engineering. However, the aesthetic outcome of chondrocyte-based microtia reconstruction is still not satisfactory. In this study, we aimed to fabricate the chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold for microtia reconstruction. We designed a unique auricular mold that could fabricate a fibrin gel scaffold resembling human auricle anatomy. Primary chondrocytes were harvested from rabbit auricular cartilage, and chondrocyte cell sheets were developed. Chondrocyte-microtissues were prepared from the cell sheets. The mixture of chondrocytes/chondrocyte-microtissues was laden in fibrin gel during the auricular scaffold fabrication. The protrusions and recessed structure in the auricular scaffold surface were still clearly distinguishable. After a one-week in vitro culture, the 3 D structure and auricular anatomy of the scaffold were retained. And followed by eight-week subcutaneous implantation, cartilaginous tissue was regenerated in the artificial auricular structure as indicated by the results of H&E, Toluidine blue, Safranin O, and type II collagen (immunohistochemistry) staining. Protrusions and depressions of the auricular scaffold were slightly deformed, but the overall auricular anatomy was maintained after 8-week in vivo implantation. Extracellular matrix components content were similar in artificial auricular cartilage and rabbit native auricular cartilage. In conclusion, the mixture of chondrocytes/chondrocyte-microtissues laden fibrin gel auricular scaffold showed a promising potential for cartilaginous tissue regeneration, suggesting this as an effective approach for autologous chondrocyte-based microtia reconstruction.

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Section: Introductionmentioning

confidence: 99%