Composite nanofibers incorporating alpha lipoic acid and atorvastatin provide neuroprotection after peripheral nerve injury in rats

Haidar, Mohammad Karim; Timur, Selin Seda; Kazanci, Atilla; Türkoğlu, Ömer Faruk; Gürsoy, R. Neslihan; Nemutlu, Emirhan; Sargon, Mustafa F.; Bodur, Ebru; Gok, Muslum; Ulubayram, Kezban; Öner, Levent; Eroğlu, Hakan

doi:10.1016/j.ejpb.2020.05.032

Cited by 18 publications

(12 citation statements)

References 47 publications

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“…Animals that survived for more than 42 days underwent the grid walking test, the open field test, and footprint analysis as previously described. [13][14][15] The behavioral tests were performed by 2 trained investigators who were blinded to the animal grouping information.…”

Section: Behavioral Assessmentsmentioning

confidence: 99%

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

et al. 2022

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Background Inflammatory reactions induced by spinal cord injury (SCI) are essential for recovery after SCI. Atractylenolide III (ATL‐III) is a natural monomeric herbal bioactive compound that is mainly derived in Atractylodes macrocephala Koidz and has anti‐inflammatory and neuroprotective effects. Objective Here, we speculated that ATL‐III may ameliorate SCI by modulating microglial/macrophage polarization. In the present research, we focused on investigating the role of ATL‐III on SCI in rats and explored the potential mechanism. Methods The protective and anti‐inflammatory effects of ATL‐III on neuronal cells were examined in a rat SCI model and lipopolysaccharide (LPS)‐stimulated BV2 microglial line. The spinal cord lesion area, myelin integrity, and surviving neurons were assessed by specific staining. Locomotor function was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale, grid walk test, and footprint test. The activation and polarization of microglia/macrophages were assessed by immunohistofluorescence and flow cytometry. The expression of corresponding inflammatory factors from M1/M2 and the activation of relevant signaling pathways were assessed by Western blotting. Results ATL‐III effectively improved histological and functional recovery in SCI rats. Furthermore, ATL‐III promoted the transformation of M1 into M2 and attenuated the activation of microglia/macrophages, further suppressing the expression of corresponding inflammatory mediators. This effect may be partly mediated by inhibition of neuroinflammation through the NF‐κB, JNK MAPK, p38 MAPK, and Akt pathways. Conclusion This study reveals a novel effect of ATL‐III in the regulation of microglial/macrophage polarization and provides initial evidence that ATL‐III has potential therapeutic benefits in SCI rats.

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Section: Behavioral Assessmentsmentioning

confidence: 99%

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

et al. 2022

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“…Haidar et al [ 194 ] used chitosan nanoparticles loaded with atorvastatin suspended in a solution of poly(L-lactide-co-glycolide) (PLGA) that contained α-lipoic acid to produce a dual therapeutic system based on electrospun nanofibers for peripheral nerve-injury treatment. The rat’s sciatic nerve injury model was assessed with motoric functions recovery and ultrastructural and biochemical analyses of regenerated nerve tissue.…”

Section: Potential Applications Of Hybrid Multifunctional Nanofibersmentioning

confidence: 99%

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

et al. 2022

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Electrospun hybrid nanofibers, based on functional agents immobilized in polymeric matrix, possess a unique combination of collective properties. These are beneficial for a wide range of applications, which include theranostics, filtration, catalysis, and tissue engineering, among others. The combination of functional agents in a nanofiber matrix offer accessibility to multifunctional nanocompartments with significantly improved mechanical, electrical, and chemical properties, along with better biocompatibility and biodegradability. This review summarizes recent work performed for the fabrication, characterization, and optimization of different hybrid nanofibers containing varieties of functional agents, such as laser ablated inorganic nanoparticles (NPs), which include, for instance, gold nanoparticles (Au NPs) and titanium nitride nanoparticles (TiNPs), perovskites, drugs, growth factors, and smart, inorganic polymers. Biocompatible and biodegradable polymers such as chitosan, cellulose, and polycaprolactone are very promising macromolecules as a nanofiber matrix for immobilizing such functional agents. The assimilation of such polymeric matrices with functional agents that possess wide varieties of characteristics require a modified approach towards electrospinning techniques such as coelectrospinning and template spinning. Additional focus within this review is devoted to the state of the art for the implementations of these approaches as viable options for the achievement of multifunctional hybrid nanofibers. Finally, recent advances and challenges, in particular, mass fabrication and prospects of hybrid nanofibers for tissue engineering and biomedical applications have been summarized.

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“…The authors considered that Sciatic Functional Index (SFI), BBB score, Extensor Postural Thrust Test (EPT), Withdrawal Reflex Latency (WRL), ultrastructural examination, and measures of TNF- α , IL-1 β , and IL-6 levels showed better results for the ALA/ATR group than other formulations and the control group. Also, it should be mentioned that all ATR groups suppressed the inflammation factors well [ 44 ].…”

Section: Neuroprotective Effectmentioning

confidence: 99%

“…Composite nanofibers containing ALA- and ATR-loaded chitosan nanoparticles (f). ATR-loaded chitosan nanoparticles existing in the composite nanofiber structure; reproduced with permission from [ 44 ].…”

Section: Figurementioning

confidence: 99%

Harnessing Therapeutic Potentials of Statins Using Nanofibrous Carriers

Ataei

Roufogalis

Kesharwani

et al. 2021

Bioinorganic Chemistry and Applications

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Statins are a wide category of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitor drugs extensively prescribed for hypercholesterolemia. In fact, many studies showed beneficial effects of these agents on a variety of related illnesses, which include increased atherosclerotic plaque stability, decreased proliferation of vascular smooth muscle, platelet aggregation, the dampening of vascular inflammation, and also anabolic effects on bone tissue. Therefore, these drugs are considered as pleiotropic agents having different clinical applications other than those for which they were initially developed. Controlled drug delivery is an efficient way of delivery in tissue engineering. Amongst different controlled release formulations, nanofibers are a novel, alternative, widely used agent because of their unique properties. These include their sustained release of drug, a high drug-loading capacity, flexible shapes with a high surface-to-volume ratio, and superior porosity. Electrospinning is an economic and a simple method employed to produce nanofibers. In this report, studies related to statin nanofiber applications have been reviewed and their results have been summarized. Four different applications of statin nanofibers have been reported, including bone generation, endothelial stenosis and thrombosis, peripheral nerve injury, and anti-inflammatory action. Studies carried out both in vitro and in vivo showed effectiveness of statins in bone healing, aneurysm, and the healing of sciatic nerve injury. In addition, statins showed apoptosis effects and anti-inflammatory effects, with dose-dependent reduction of IL-6 and dose-independent reduction of TNF-α. Despite these promising results, validation via clinical trials is yet to be performed. The scope of statins in their pleiotropic range of actions is still not completely explored, and studies are still needed to enlighten different useful aspects of such drugs.

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Composite nanofibers incorporating alpha lipoic acid and atorvastatin provide neuroprotection after peripheral nerve injury in rats

Cited by 18 publications

References 47 publications

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

Harnessing Therapeutic Potentials of Statins Using Nanofibrous Carriers

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