Brychta P., D. Horky, D. Havranova, Z. Janecek, 1. Adler: Model Skin DeJects in Rats Jor the Composite Skin Graft Evaluation. Acta vet. Bmo, 1997,66:23-26. An animal model (rat) was used for the study of the wound healing process. Full-thickness round skin defects, 3 cm in diameter, placed on the dorsal side of the body were made down to muscle fascia. Plastic rings were used to prevent wound contraction and spontaneous epithelialization from the wound edges.In the first step allodermis was prepared using trypsin, and applied into the wound bed. Cultured epidermal autografts were prepared in vitro using modified Rheinwald-Green method, and in the second step applied on the taken allodermis. In this way composite skin grafts consisting of allodermis and cultured epidermal autografts were constructed. The semipermeable dressing was changed three-times a week.The healing process was monitored using both the appearance and light microscopic evaluation. In the experimental group of 10 animals uniform uncomplicated healing of these composite skin grafts occurred.After 3 months, the composite skin grafts acquired a form very similar to intact skin missing only the appendages (hair and glands). In the microscopic view only rather flat rete ridges under the dermo-epidermal junction made the difference between the graft and intact skin.
Model skin dejects, cultured epidermal autografts, allocorium, composite skin graftsThe substitution of the skin damaged in its full thickness due to injury, especially bum, has not been resolved quite satisfactorily. Keratinocytes cultured in vitro represent a hopeful perspective for solving this problem (Rheinwald and Green 1975;Green et al. 1979). However, it seems that a mere substitution of the epidermis is not quite satisfactory because a good-quality substitution also requires a corial component (Compton et al. 1989;Schiozer et al. 1994).The aim of the present study was therefore to approach this problem by recombining allogeneic corium and autologous cultured keratinocytes used for covering of a fullthickness skin defect. A rat experimental model was employed.
Materials and MethodsTen white laboratory non-inbred female rats, 6 months old, 300-400 g body mass, were used for the experiment (four intact rats were used as the control group).Surgical interventions were performed in general anaesthesia with 0.2 ml Rometar 2% + 0.8 ml Narkoman 5%.kg-1 body mass.A skin sample for keratinocyte cultivation was taken from the right inguinum of a rat. Hair was removed from the skin. the skin was disinfected with "Spitaderm TM", a sample in its full-thickness, 2 x 1 em, was excised in a sterile way and transported into laboratory in Dulbecco -Minimal Essential Medium (D-MEM) with antibiotics. The skin defect was simply sutured.After removal from the transport medium, the skin sample was placed into 0.2% solution of trypsin and incubated at 4 °C for 24 hours. Then it was washed with Phosphate Buffered Saline (PBS), and keratinocytes were loosened mechanically from the corium in a s...