Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins

Hajizadeh, Solmaz; Kirsebom, Harald; Leistner, André; Mattìasson, Bo

doi:10.1002/jssc.201200433

Cited by 34 publications

(23 citation statements)

References 36 publications

(33 reference statements)

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“…Due to their interconnected, macroporous 3D structure, cryogels have been widely used in bioseparation‐related applications [1–10], tissue engineering [10–14], and other relevant bioengineering and sensor applications [15–17]. In particular, monolithic cryogels have been used as a new generation of chromatographic matrices for the separation of cells (mammalian, bacterial, and yeast), proteins, viruses, and plasmids [18–22]. Benefitting from their large and interconnected pores, cryogel monoliths are capable of processing nonclarified, viscous feedstreams, including blood, plasma, and fermentation broth, alongside plant and animal extracts [23,24], which could not be processed by traditional chromatographic mediums involving packed beads without high backpressures or clogging [25–27].…”

Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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A novel, facile, and robust strategy was proposed to increase the pore size and mechanical strength of cryogels. By mixing the monomers of acrylamide and 2‐hydroxyethyl methacrylate as the precursor, a monolithic copolymer cryogel with large interconnected pores and thick pore walls was prepared. Hydrogen bonding between the two monomers contributed to the entanglement and aggregation of the copolymers, thickening the pore walls and resulting in larger pore sizes. Analysis via mercury porosimetry demonstrated that the interconnected pore diameter of the copolymer cryogel ranged from 10‐350 µm, which was far larger than that of the cryogels from one monomer (10‐50 µm). Additionally, the thicker pore walls of the copolymer cryogel improved its mechanical strength. Affinity cryogels were prepared through covalent immobilization using Tris(hydroxymethyl)aminomethane as a coupling agent, and the affinity binding of lysozymes on Tris‐cryogel was evaluated by the Langmuir isothermal adsorption with the maximum adsorption capacity of 360 mg/g. Compared with that of the Tris‐cryogels produced from one monomer, the copolymer Tris‐cryogel exhibited higher adsorption capacity and lysozyme purity, when the chicken egg white solution flowed solely driven by gravity. This work provides a new avenue for designing and developing supermacroporous cryogels for bioseparation.

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Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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show abstract

“…High back-pressure is not a problem for cryogel columns, whereas it is an important problem for conventional columns composed of microspheres. However, big pores bring along a disadvantage, which is low surface area to cryogels that results in low binding capacity of cryogel column (Yao et al, 2007;Baydemir et al, 2009;Aslıyüce et al, 2010;Koç et al, 2011;Hajizadeh et al, 2012). Composite cryogels increase the surface area by embedding microspheres because of high surface area of microspheres (Yao et al, 2006;Le Noir et al, 2007;Bereli et al, 2010;Ivanov et al, 2012;Sun et al, 2012a;Sun et al, 2012b).…”

Section: Introductionmentioning

confidence: 97%

Molecularly imprinted composite cryogels for hemoglobin depletion from human blood

Baydemir

Andaç

Perçin

et al. 2014

J of Molecular Recognition

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A molecularly imprinted composite cryogel (MICC) was prepared for depletion of hemoglobin from human blood prior to use in proteome applications. Poly(hydroxyethyl methacrylate) based MICC was prepared with high gel fraction yields up to 90%, and characterized by Fourier transform infrared spectrophotometer, scanning electron microscopy, swelling studies, flow dynamics and surface area measurements. MICC exhibited a high binding capacity and selectivity for hemoglobin in the presence of immunoglobulin G, albumin and myoglobin. MICC column was successfully applied in fast protein liquid chromatography system for selective depletion of hemoglobin for human blood. The depletion ratio was highly increased by embedding microspheres into the cryogel (93.2%). Finally, MICC can be reused many times with no apparent decrease in hemoglobin adsorption capacity.

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“…Several types of monomers are used to produce these cryogels all with similar properties. Commonly used monomers are acrylamide , 2‐hydroxyethyl methacrylate , and poly(vinylalcohol) . On the other hand, a disadvantage of the cryogel is its low adsorption capacity .…”

Section: Introductionmentioning

confidence: 99%

Development and screening of epoxy‐spacer‐phage cryogels for affinity chromatography: Enhancing the binding capacity

Noppe

Deckmyn

2017

J of Separation Science

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Macroporous epoxy cryogels can be used as an alternative for classical matrices in affinity chromatography. Due to the structural properties of cryogels, with pores of up to 100 μm, crude samples can be processed at high speed without previous manipulations such as clarification or centrifugation. Also, we previously used a peptide-expressing M13 bacteriophage as an affinity ligand. These ligands show high specificity toward the target to be purified. Combination of both, leads to a relative cost-effective one-step chromatographic set-up delivering a high purity sample (>95%), however, so far with limited capacity. To increase the binding capacity of the affinity columns, we now inserted spacers between the chromatographic matrix and the phage ligand. Both linear spacers, di-amino-alkanes (C -C ), and branched polyethyleneimine spacers with different molecular weights (800 Da-10 kDa) were analyzed. Two types of peptide expressing phage ligands, a linear 15-mer and a cyclic 6-mer, were used for screening. Up to a tenfold increase in binding capacity was observed depending on the combination of phage ligand and spacer type.

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Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins

Cited by 34 publications

References 36 publications

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Molecularly imprinted composite cryogels for hemoglobin depletion from human blood

Development and screening of epoxy‐spacer‐phage cryogels for affinity chromatography: Enhancing the binding capacity

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