Components of the gaseous environment and their effects on plant growth and development in vitro

Buddendorf-Joosten, J. M. C.; Woltering, E.J.

doi:10.1007/bf00024671

Cited by 107 publications

(19 citation statements)

References 83 publications

(109 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These plantlets usually need elevated CO 2 concentration and higher irradiance than conventionally used. 25,[50][51][52]65,66 Our results are in conformity with the above statement. In all the plant systems maximum F v / F m and ΦPS2 were obtained at either 10.0 or 40.0 gm -3 of CO 2 both in SFSM and SFLM.…”

Section: Discussionsupporting

confidence: 91%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

View full text Add to dashboard Cite

Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

show abstract

Section: Discussionsupporting

confidence: 91%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

View full text Add to dashboard Cite

show abstract

“…These undesirable compounds can alter biochemical responses and leaf development of in vitro cultured plants (Pierik et al [ 2007 ]) and also affect enzymes involved in oxidative activities (Synková and Pospíšilová [ 2002 ]). Some closures cause restriction of gaseous exchange between the container atmosphere and the outside environment (Buddendorf-Joosten and Woltering [ 1994 ]), which can result in poor aeration and hyperhydric condition of cultures. Also, growth rate and other physiological and morphological characteristics of plants developed under in vitro conditions can be influenced by the physical and chemical micro-environments of culture containers (Walker et al [ 1988 ]).…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

Huang

Agrawal

et al. 2014

Bot Stud

View full text Add to dashboard Cite

BackgroundGentiana scabra Bunge commonly known as ‘Long dan cao’ in China has been used in traditional Chinese medicines for more than 2000 years. Dry roots and rhizome of the herb have been used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are the main bioactive compounds which attribute to the pharmacological properties of this plant. The species is difficult to mass propagate by seed due to the low percentage of germination and limited dormancy period. Wild populations in some locations are considered to be in the endangered category due to over exploitation.ResultsIn the present study, we report an efficient micropropagation system. Shoot apices of six weeks old in vitro grown G. scabra plants were used as explants for the in vitro propagation. Induction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog’s basal medium (MSBM) containing 2.0 mg/L−1 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar. In vitro shoots induced profuse rooting on half strength of MSBM supplemented with 0.1 mg/L−1 1-naphthaleneacetic acid (NAA), 3% sucrose and 0.3% gelrite. A two-stage ventilation closure procedure during the in vitro culture, and transparent sachet technique enhanced the survival rate of G. scabra plantlets to 96% in the greenhouse. Tissue culture plants flowered after 5 months of transfer to pots.ConclusionsA simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments has been developed. The protocol can be very useful in germplasm conservation and commercial cultivation of G. scabra plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-014-0056-4) contains supplementary material, which is available to authorized users.

show abstract

“…Ethylene is suggested to mediate many auxin and cytokinin responses (1) and affect gene expression (13). Ethylene effects on tissue culture vary 13 with the species, explants and processes studied (10,14,61).…”

Section: Introductionmentioning

confidence: 99%

Ethylene Impact on Somatic Embryogenesis: Biotechnological Considerations

Kuklin

1995

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The gaseous plant hormone ethylene affects somatic emb1yogenesis in various ways depending on the species or explants used. Inclusion of ethylene action inhibitors, name~v silver ions in culture media increased somatic embryogenesis in some species. Large scale (bioreactor) yields of somatic emb1yos were less than these ji·01n flask cultures. Ethylene depletion from the bioreactors due to aeration is considered as a possible reason for the decrease in somatic emb1yogenesis. Ethylene is discussed as a physiological marker related to emb1yogenic potential. ~Vound ethylene evolved after transformation experiments might have some inhibit01:v role on transformation but results are contradictory. Ethylene plays different roles in long term storage (low and ambient temperatures) of emb1yogenic cultures. Use of transgenic technology in ethylene biosynthesis reduction is discussed as a way to understanding ethylene role in somatic emb(vogenesis.

show abstract

Components of the gaseous environment and their effects on plant growth and development in vitro

Cited by 107 publications

References 83 publications

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

Ethylene Impact on Somatic Embryogenesis: Biotechnological Considerations

Contact Info

Product

Resources

About