Components in seminal plasma regulating sperm transport and elimination

Troedsson, Mats H.T.; Desvousges, A.; Alghamdi, Abdorrahman S.; Dahms, Beverly B.; Dow, Charlotte; Hayna, J.; Valesco, R.; Collahan, P.T.; Macpherson, M. L.; Pozor, Małgorzata A.; Buhi, William C.

doi:10.1016/j.anireprosci.2005.07.005

Cited by 112 publications

(68 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Addition of SP to preserved semen immediately before insemination may be beneficial for some reasons: SP modulates the immune response in the female genital tract to protect spermatozoa (Troedsson et al, 2000(Troedsson et al, , 2005Dahms & Troedsson, 2002;Alghamdi et al, 2004;Doty et al, 2011) and promotes elimination of non-viable spermatozoa (Troedsson et al, 2005); SP is able to increase immediate motility of fresh and frozen-thawed epididymal and ejaculated spermatozoa (Braun et al, 1994;Stout et al, 2000;Heise et al, 2011;Neuhauser et al, 2013;Morrell et al, 2014); SP seems to be important for sperm maturation, plasma membrane remodelling, capacitation, acrosome reaction and fertilization (T€ opfer-Petersen et al, 2005;Rodr ıguez-Mart ınez et al, 2011;Caballero et al, 2012;Barrier-Battut et al, 2013) and could therefore increase fertility of epididymal spermatozoa. Although adding SP to the flushing medium did not improve post-thaw motility of epididymal spermatozoa in all stallions (Heise et al, 2011), there was a significant improvement in motility when added after the freeze-thaw process (Neuhauser et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

View full text Add to dashboard Cite

SUMMARYPreservation of epididymal spermatozoa is important to save genetic material of endangered species and breeds, or in case of unexpected injury, which will end the breeding career of valuable sires. Seminal plasma (SP) influences sperm quality in a dosedependent manner and its addition to preserved semen immediately before insemination may be beneficial for sperm fertility. Increased plasma membrane stability of epididymal spermatozoa reduces freezing injury of cells, and the addition of SP after freezing and thawing might have activating and protecting effects on spermatozoa within the female genital tract. In this study, epididymal spermatozoa were harvested by retrograde flush of the epididymal cauda immediately after routine castration and frozen. Seminal plasma was collected from other six stallions. Homologous SP (SP from the same species, but from a different animal) was added to frozen-thawed epididymal spermatozoa at concentrations of 0, 5, 20, 50 and 80% SP. Addition of SP increased sperm motility and influenced kinematic values in a dose-dependent manner (p < 0.05). Motility improved at concentrations of 20 and 50% SP, but did not further increase at 80% SP. There was no difference in sperm motility among SP from six different donor stallions regardless of the concentrations of SP (p > 0.05). Total and progressive motility of ten frozen-thawed epididymal spermatozoa samples collected from different stallions after dilution with extender and 5, 20, 50 or 80% SP differed significantly (p < 0.05). In conclusion, addition of homologous SP to frozen-thawed stallion epididymal spermatozoa immediately improved motility in a dose-dependent manner regardless of semen quality of SP donor stallions. This might positively influence fertility when SP is added before insemination. Moreover, there seems to be a threshold level of SP concentration for optimal improvement of sperm motility.

show abstract

Section: Introductionmentioning

confidence: 99%

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

View full text Add to dashboard Cite

show abstract

“…Even though the mare has a physiological heavy inflammatory reaction, known as post-breeding induced endometritis, shortly after insemination due to the presence of sperm (Troedsson et al 2005), this reaction is seldom detected as an increase in the degree of endometrial edema in the normal mare at 24 to 48 hrs post-breeding.…”

Section: Dealing With Edemamentioning

confidence: 99%

A review of a practitioner’s perspective on endometrial edema

Samper¹

2010

PHK

View full text Add to dashboard Cite

“…Less than 1% of inseminated spermatozoa are successfully transported to the oviducts after insemination (Scott et al 1995, Bader andKrause 1980). Preliminary data suggests that prostaglandin E (PGE 2α ) may be involved in sperm transport from the uterus to the oviduct (Troedsson et al 2005). We have previously observed that PGE 2α causes smooth muscle contractions in the equine oviduct (Troedsson et al 1995b).…”

Section: Transport Across the Utero-tubal Junctionmentioning

confidence: 99%

Interaction between spermatozoa and the mare’s reproductive is mediated by biochemical, physical, and inflammatory mechanisms

Troedsson¹

2006

PHK

View full text Add to dashboard Cite

SummarySperm transport and elimination from the mare's reproductive tract are important components of the events that lead to fertilization and successful development of an embryo. Following deposition of semen into the uterine body, viable spermatozoa are transported to the oviduct by a combination of sperm motility, uterine contractility, and epithelial cilia activity. This process is facilitated by the release of oxytocin from the pituitary, as well as prostaglandins and estrogen in seminal plasma. The myoelectrical activity in the uterus shows a two-wave pattern following AI. The first immediate phase of contractions is believed to be involved in both sperm transport to the oviduct, and elimination of excess spermatozoa through the cervix. The second phase of contractions coincides with an inflammatory response to spermatozoa. The inflammation is an important physiological component of sperm elimination from the mare's reproductive tract. It is regulated by seminal components. Spermatozoa activate complement in uterine secretion, resulting in chemotaxis of PMNs into the uterine lumen. The PMNs bind to spermatozoa that consequently are removed from the uterus by phagocytosis. Inflammatory mediators, such as PGF2α, cause contraction of the myometrium, and residual spermatozoa, accumulated fluid, and harmful inflammatory products are removed by this second wave of uterine contractions. Once these products are removed from the uterine lumen, the inflammation subsides and the uterine environment returns to its normal state. Specific seminal plasma proteins modulate breeding-induced endometritis, and appear to selectively protect viable spermatozoa from binding to PMNs.Keywords: mare, post-breeding-endometritis, uterine clearance, spermatozoa, inseminate components Der Einfluss von biochemischen, physikalischen und entzündlichen Mechanismen bei den Interaktionen zwischen Spermatozoen und dem Genitaltrakt der Stute Der Transport der Spermien und ihre Elimination aus dem Stutengenitale stellen wichtige Komponenten innerhalb der Vorgänge dar, die zur Konzeption und zur erfolgreichen Entwicklung des Embryos führen. Nach dem Verbringen des Samens in die Gebärmutter werden lebensfähige Spermatozoen in die Eileiter transportiert. Dies geschieht durch eine Kombination von Spermienmotilität, uterinen Kontraktionen und epithelialer Zilienaktivität. Dieser Prozess wird unterstützt durch die Oxytocinfreisetzung aus der Hypophyse sowie durch Prostaglandine und Östrogen im Seminalplasma. Die myoelektrische Aktivität des Uterus weist nach der instrumentellen Samenübertragung eine zweifache Wellenbewegung auf. Die erste, sofort einsetzende Kontraktionsphase ist vermutlich sowohl beim Spermientransport in den Eileiter als auch bei der Elimination überschüssiger Spermatozoen durch die Zervix involviert. Die zweite Phase der Kontraktionen trifft mit der Entzündungsreaktion auf die Spermatozoen zusammen. Die Entzündung ist ein wichtiger physiologischer Bestandteil bei der Spermienelimination aus dem Stutengeniale. Sie wird durch die ...

show abstract

Components in seminal plasma regulating sperm transport and elimination

Cited by 112 publications

References 28 publications

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

A review of a practitioner’s perspective on endometrial edema

Interaction between spermatozoa and the mare’s reproductive is mediated by biochemical, physical, and inflammatory mechanisms

Contact Info

Product

Resources

About