Component-resolved diagnostics in Thai children with cow’s milk and egg allergy

Jessadapakorn, Wipa; Sangsupawanich, Pasuree; Wootipoom, Natthakul; Suddeaugrai, Oranart; Yuenyongviwat, Araya

doi:10.12932/ap0820

Cited by 6 publications

(5 citation statements)

References 8 publications

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“…34,55 Studies on the sensitivity profiles of Thai children with CMA indicate that most were sensitive to βLG and casein. 72 Similarly, a Japanese study found that 97% of children with these demonstrate sensitivity of 63 and 61% and specificity of 87 and 83%, respectively. No other milk components were found to give clinically relevant results.…”

Section: Casein Proteinsmentioning

confidence: 94%

“…Another example of the use of CRD in determining the phenotype of the disease may be that given by Jessadapakorn et al, 72 in which higher casein levels were observed in children with urticaria compared to children CMA who acquired tolerance before the age of four compared to healthy children. Instead, they showed that high levels of milk components and milk sIgE increase the risk of long-lasting or persistent CMA.…”

Section: Referencesmentioning

confidence: 99%

“…At the same time, the attempt to assess the significance of specific-to-total IgE ratios did not bring any results. 29 Research in Europe, the United States, China, Japan, and Thailand 27,72,73,76,77 has shown that of all milk components, casein was responsible for the most severe allergic reactions. In contrast, αLA demonstrated the weakest allergenicity and antigenicity.…”

Section: Referencesmentioning

confidence: 99%

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The importance of component-resolved diagnostics in IgE-mediated cow's milk allergy

Popielarz

Krogulska

2021

aei

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Cow’s milk allergy (CMA) is an increasingly common problem among children and adults that requires the use of appropriate diagnostics to eliminate allergic reactions and prevent unnec-essary dietary regimes. The current diagnostics methods are imperfect hence new, more effective methods are still being sought. Component-resolved diagnostics (CRD) is one of them. CRD assesses sensitivity to individual allergen molecules using purified native or recombinant allergens. The present paper reviews the role of CRD in diagnosing CMA, as well as the benefits and limitations of its use, especially in predicting allergy development or acquiring immunotolerance. It examines the possibility of replacing the current gold diagnostic standard with component tests directed against specific milk proteins. In addition, CRD could be helpful in the evaluation of prognosis. However, CRD allows for improvement in clinical management, particularly of polysensitized subjects, there is still no cogent evidence that it offers more efficient CMA diagnostics than existing tests.

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Section: Casein Proteinsmentioning

confidence: 94%

Section: Referencesmentioning

confidence: 99%

Section: Referencesmentioning

confidence: 99%

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The importance of component-resolved diagnostics in IgE-mediated cow's milk allergy

Popielarz

Krogulska

2021

aei

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“…However, the use of crude allergen extracts reduces the reliability of those tests since they often contain a mixture of allergenic and nonallergenic components. The difficulties associated with crude allergen extracts can be easily overcome by the use of component-resolved diagnosis (CRD) [ 7 ]. In CRD, specific allergens are used for measurement of sIgE [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Immunological Comparison of Native and Recombinant Hen’s Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis

et al. 2018

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Chicken serum albumin (CSA) is a hen’s egg yolk allergen causing IgE-mediated allergy. The objective of this study was to produce a recombinant version of CSA and compare its IgE reactivity to natural CSA (nCSA). CSA was cloned and expressed as a soluble fraction in the yeast Kluyveromyces lactis (K. lactis) protein expression system. The gene encoding CSA was amplified with a C-terminal hemagglutinin epitope tag by polymerase chain reaction (PCR) and cloned into the pKLAC2 expression vector prior to transforming into K. lactis. Recombinant CSA (rCSA) was purified by immunoprecipitation. Twenty-one patients allergic to hen’s egg white were examined for sensitisation against nCSA. 38% of patients were found to be sensitised to CSA based on Western immunoassay. Immunoglobulin E (IgE) binding capacity of rCSA and nCSA was analysed by ELISA using sera from patients sensitised to CSA. Levels of IgE-binding were similar for both the recombinant and the natural CSA, indicating the existence of similar epitopes. rCSA produced in this study is a potential candidate to be used in component-resolved diagnosis (CRD) of egg yolk allergy. The usefulness of rCSA in CRD of egg yolk allergy warrants further characterisation using sera from patients with allergy to hen’s egg yolk in future studies.

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“…Another study of children with CMA showed that the positive rates of β-lactoglobulin and casein were the highest, followed by a-lactalbumin. [ 10 ] Therefore, β-lactoglobulin might be a more sensitive indicator of CMA.…”

mentioning

confidence: 99%