Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies

Zhu, Liang; Qi, Hanying; Lv, Mei-le; Kong, Yun; Yu, Yanwen; Xu, Xiangyang

doi:10.1016/j.biortech.2012.08.059

Cited by 334 publications

(98 citation statements)

References 33 publications

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“…A steady-state condition was reached after 65 days. Maximum PS and PN production was observed at the end of the feast period, as observed also by Zhu et al (2012). PS and PN concentrations reached their maximum values, when most of the organic substrate was removed from the bulk (feast phase), after 20 and 45 min on average, in R1 and R2 respectively.…”

Section: Physical Characteristics Of Aerobic Granulessupporting

confidence: 69%

“…As reported by Zhu et al (2012) in this phase, the microorganisms began to adjust their metabolisms to the new feed and high shear forces, so the EPS content grew rapidly. In this period the first bioaggregates appeared in the reactor.…”

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 87%

“…The large granule sizes and their density in fact, may constitute a barrier to nutrient diffusion inside the inners layers, so microorganisms in the absence of primary substrate used carbohydrates instead of proteins due to their simpler molecular structure. Zhu et al (2012) in their study reported that about 50% of proteins and 47% of carbohydrates were consumed during the starvation phase. In this case the difference in protein reduction is due to the shorter duration of the starvation phase (2.5 h vs 10 h); specifically, it is possible to note that in the first phase of the famine period, the microorganisms preferentially consume storage PS, using the PN only at a later time.…”

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 97%

“…As a consequence, the EPSs content was modified during the reaction cycle. Few authors reported EPSs variation during a reaction cycle (Zhu et al, 2012), but no information are available about their changes during the whole granulation process.…”

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 99%

“…The results obtained are in agreement with Taheri et al (2012) and Wan et al (2014), who observed that under high saline conditions microorganisms produce more EPS, to regulate their osmotic pressure with the outside. With PN content increasing, the sludge surface properties underwent a modification; the positive charges of the amino groups reduced the sludge surface electronegativity (Zhu et al, 2012). In addition, proteins bonding with the metal ions and oily particles content in influent wastewater could also reduce cell surface electronegativity, thus contributing to microbial aggregation.…”

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 99%

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Cultivation of granular sludge with hypersaline oily wastewater

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et al. 2015

International Biodeterioration & Biodegradation

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a b s t r a c tThe time required to stabilise mature aerobic granules is rather variable. In addition, cultivation time and the structural characteristics of granules seem to be related to the nature of wastewater influent. Granular sludge has been used for the treatment of several industrial wastewaters, but nothing has been reported about wastewater characterized by the simultaneous presence of hydrocarbons and high chloride concentration. In this work, the authors analysed the granulation process and performance as well as the physical characteristics of aerobic granules in two Granular Sequencing Batch Airlift Reactors (GSBARs), fed with acetate-based synthetic wastewater in reactor 1 (R1) and with a mixture of real and simulated slop (R2). The results obtained in 100 days show that full granulation was achieved in both reactors. The granules in R2 developed more quickly, but they appeared slightly unstable and more susceptible to breaking. Despite high salt concentration, the efficiency of phosphorous and carbon removal was satisfactory. Low nitrification activity was observed in R1, confirming that a longer time is necessary to obtain the acclimation of autotrophic biomass in aerobic granules. In R2 the combined effect of salinity and hydrocarbons caused the inhibition of the autotrophic biomass, with the consequence that nitrification was absent. Hydrocarbons were initially removed by adsorption afterwards by biological degradation with a removal efficiency of over 90%.

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Section: Physical Characteristics Of Aerobic Granulessupporting

confidence: 69%

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 87%

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 97%

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 99%

Section: Physical Characteristics Of Aerobic Granulesmentioning

confidence: 99%

See 3 more Smart Citations

Cultivation of granular sludge with hypersaline oily wastewater

Corsino

Campo

Bella

et al. 2015

International Biodeterioration & Biodegradation

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Effect of microbiological inoculants DN‐1 on lignocellulose degradation during co‐composting of cattle manure with rice straw monitored by FTIR and SEM

Liu

et al. 2015

Env Prog and Sustain Energy

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Lignocellulose is the uppermost limiting factor influencing the maturity process of agricultural waste compost. The purpose of this study was to determine the promotion of lignocellulose degradation by the inoculants DN‐1 during co‐composting of cattle manure with rice straw. Lignocellulose content, functional groups, and microscopic structure were investigated by the chemical determination, the Fourier transform infrared spectroscopy (FTIR) analysis, and the scanning electron microscopy (SEM) observation, respectively. The chemical determination indicated that the inoculants DN‐1 promoted the degradation of cellulose and hemicellulose in the initial stage of composting and the degradation of lignin in the later thermophilic stage. After composting, the degradation rate of cellulose, hemicelluloses, and lignin in the inoculated compost was 62.57%, 67.14%, and 42.54% respectively, while those in the natural compost was only 31.27%, 47.57%, and 22.21%, respectively. During composting, in the inoculated compost, the characteristic absorption peaks of cellulose and hemicellulose (at 3312, 2930, 1050, and 895 cm−1) and lignin (at 1506 cm−1) decreased noticeably, while in natural compost, except for the peak at 1050 cm−1, no obvious changes in these peaks were observed, confirming that the inoculants were effective in promoting the degradation of lignocellulose. The SEM images provided clear morphological evidence of lignocellulose degradation during composting. After composting, the serious degradation of parenchyma tissue (which is composed of cellulose and hemicellulose mainly) and sclerenchyma tissue (which is composed of lignin mainly) was observed in the inoculated compost. While in the natural compost, only the partial degradation of parenchyma tissue occurred, no obvious degradation of sclerenchyma tissue was found at the end of composting. © 2015 American Institute of Chemical Engineers Environ Prog, 35: 345–351, 2016

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Effects of hexavalent chromium on performance, extracellular polymeric substances and microbial community structure of anaerobic activated sludge in a sequencing batch reactor

Liu

Jin

Liu

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: Bioremediation of Cr(VI) to Cr(III) was considered a promising and cost-effective alternative to chemical and physical methods. However, Cr(VI) had toxic effects on microorganisms. This study focused on exploring the effects of different Cr(VI) concentrations on the performance of an anaerobic activated sludge reactor, extracellular polymeric substances (EPS) and microbial community structure. RESULTS: Anaerobic sludge reduced and immobilized 100.0 mg L −1 Cr(VI) in 24 h. When the Cr(VI) concentration was increased from 0.0 to 50.0 mg L −1 , the production of EPS was enhanced from 231.4 to 1262.9 mg g −1 VSS. With 80.0 mg L −1 Cr(VI) and 120.0 mg L −1 Cr(VI), EPS production was inhibited. Increase of Cr(VI) concentration changed the EPS components and had distinct effects on the functional groups of EPS. In addition, the increase of Cr(VI) stress reduced the abundance of most bacteria. CONCLUSION: Low Cr(VI) concentrations had no effect on COD Cr and NO 3 -N removal, while 120.0 mg L −1 Cr(VI) seriously inhibited on COD Cr , NO 3 -N and Cr(VI) removal. This was due to the toxicity and electron competition ability of Cr(VI). High Cr(VI) concentrations (80.0 and 120.0 mg L −1 ) also inhibited EPS production. Cr(VI) reduction was closely related with EPS.

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Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies

Cited by 334 publications

References 33 publications

Cultivation of granular sludge with hypersaline oily wastewater

Cultivation of granular sludge with hypersaline oily wastewater

Effect of microbiological inoculants DN‐1 on lignocellulose degradation during co‐composting of cattle manure with rice straw monitored by FTIR and SEM

Effects of hexavalent chromium on performance, extracellular polymeric substances and microbial community structure of anaerobic activated sludge in a sequencing batch reactor

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