Component analyses of urinary nanocrystallites of uric acid stone formers by combination of high‐resolution transmission electron microscopy, fast Fourier transformation, energy dispersive X‐ray spectroscopy, X‐ray diffraction and Fourier transform infrared spectroscopy

Abstract

This study aimed to analyse the components of nanocrystallites in urines of patients with uric acid (UA) stones. X-ray diffraction (XRD), Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy (HRTEM), fast Fourier transformation (FFT) of HRTEM, and energy dispersive X-ray spectroscopy (EDS) were performed to analyse the components of these nanocrystallites. XRD and FFT showed that the main component of urinary nanocrystallites was UA, which contains a small amount of calcium oxalate monohydrate and phosphates. EDS showed the characteristic absorption peaks of C, O, Ca and P. The formation of UA stones was closely related to a large number of UA nanocrystallites in urine. A combination of HRTEM, FFT, EDS and XRD analyses could be performed accurately to analyse the components of urinary nanocrystallites.

Analysis of the Application Value of X-Ray Digital Tomographic Fusion Technique in Urinary System Diseases

“…Although the above two routine examination methods have their own advantages and play a very important role in the diagnosis of urinary diseases, they may be affected by overlapping images of intestinal contents and intestinal gases of the patients. As a result, the image of the anatomical structure of the urinary system is not clear enough, the location of the lesion cannot be accurately displayed, and it is difficult to achieve the diagnostic goal [24]. e use of digital tomographic fusion technology is to use a receiver, and it is fixed; through the use of single-layer imaging scanning, a series of exposure images are collected in this process, and the collected data are reconstructed scientifically, and then, start from the surface of the receiver to the imaging anatomy, and the multiple planes involved are displayed [25,26].…”

Metabolic studies and calculi analysis in urinary lithiasis, how to stop recurrence?

“…A thorough metabolic study should include two 24-hour urine collections, blood analysis and physicalchemical analysis of the stone. X-ray diffraction and infrared spectroscopy by Fourier transformation are the recommended tests for the physical study of the calculi 59,68 . These methods permit correct identification of the type of calcium stone present and other associated stone components, such as cystine, xanthine, uric acid, urates, struvite, proteins, lipids, and/or drugs 2,6 .…”

Relationship between Components of urinary crystallites and Formation of Calcium Oxalate Stones---An Investigation of 275 Cases of Patients with Calcium Oxalate Stones

“…There exists a close relation between urinary crystallites and kidney stones [5][6][7]. The disappearance of a specific type of urinary crystallites (e.g.…”