Complications of Gastroenteritis By Typhoid Fever in Amazonia: Clinical Cases Genetic Evaluation from Intestinal Drilling, Pneumonia and Cholestatic Hepatitis

Abstract

Three Salmonella Typhi bacterial isolates from blood culture and coproculture of patients with intestinal perforation, pneumonia and cholestatic hepatitis were evaluated, available at the Bacteriology and Mycology Section of the Evandro Chagas Institute (IEC). To identify the Salmonella species and its genetic profile, six virulence genes invA, viAB, prt, tcf, tyv and H-d were analyzed [3,10,11]. The DNA of the bacterial isolates was extracted using the boiling and freezing technique [12]. Each set of primers (1.25mM) was incubated in an amplification reaction with a final volume of 25µL, containing 20ng of DNA, 10Mm Tris-HCl, pH 8.5,