Complications of Disease and Therapy

Clarke‐Pearson, Daniel L.

doi:10.1016/b978-0-323-07419-3.00016-3

Cited by 1 publication

(1 citation statement)

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“…However, ECT is mostly performed on patients with cutaneous and subcutaneous tumors, and there is not adequate number of studies that can prove that there is no significant toxicity in patients with pancreatic cancer. Although toxicity incidence dramatically rises in patients receiving a dose in excess of 450 mg/m 2 , toxicity has been reported at doses of less than 100 mg and general anaesthesia following the use of bleomycin may be complicated by postoperative respiratory failure possibly secondary to a bleomycin-induced sensitivity of oxygen [ 16 ]. Since lung, liver, and peritoneum are the most common sites of metastasis [ 17 ], there is a need to implement alternative drugs for patients suffering from metastatic lung cancer and bleomycin-sensitive patients.…”

Section: Introductionmentioning

confidence: 99%

Electroporation with Cisplatin against Metastatic Pancreatic Cancer:In VitroStudy on Human Primary Cell Culture

Michel

Kulbacka

Saczko

et al. 2018

BioMed Research International

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Despite the rapid progression of cancer pharmacotherapy, the high drug resistance of pancreatic ductal adenocarcinoma (PDA) makes it one of the most lethal malignancies. Therefore, there are high expectations associated with experimental therapies, such as electrochemotherapy (ECT). This technique involves the application of short electric pulses to induce transitional permeabilization of the cellular membrane, thus enhancing drug molecules influx. The aim of the study was to investigate the influence of electroporation with cisplatin (CisEP) on the primary culture of human PDA cells from lung metastases—their survival and stress response. Considering the growing importance of various research models, two established human PDA cell lines, EPP85-181P (sensitive to daunorubicin) and EPP85-181RDB (resistant to daunorubicin), were utilized as a reference control. Cisplatin revealed higher cytotoxicity towards established cell lines. Following CisEP application, we observed a significant decrease of cells viability in the primary culture model. After CisEP therapy, an increased immunoreactivity with SOD-2 and Casp-3 antibodies was noticed. In conclusion, we discovered that electroporation can enhance the cytotoxic effect of cisplatin in pancreatic cancer cells in vitro. This effect was evident for cells from the primary culture. The obtained results confirm the importance of primary cells models in studies on the efficacy of experimental cancer therapies.

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Section: Introductionmentioning

confidence: 99%