Complexes of Angiotensin IV with Functionally Different Proteins in the Regulation of Drinking Behavior and Hemodynamics in Rats

Abstract

We compared physiological activity of synthetic complexes from angiotensin IV and functionally different proteins (transport protein, bovine serum albumin; and neurospecific Ca(2+)-binding protein, S100b) as model analogues of endogenous protein-peptide complexes. Physiological activity of angiotensin IV was specifically modified by these proteins. Our results suggest that complexes of angiotensin IV with bovine serum albumin and S100b are strong factors for the integration of central and peripheral functions at the homeostatic and behavioral level.

From Agonist to Antagonist: Modulation of the Physiological Action of Angiotensins by Protein Conjugation—Hemodynamics and Behavior

“…For example, AngII-BSA significantly enhances drinking behavior, so it is logical to assume a potentiating effect of this conjugate, similar to native AngII, on hemodynamic parameters, such as blood pressure and heart rate Notes: Hemodynamic effects: an increase (↑) or a decrease (↓) values are indicated, the percentage change in the measured parameters relative to the control is indicated in parentheses; behavioral effects: minus/plus means the absence/presence of an effect, the severity of which increases from minimum (+) to maximum (++++). The data were taken from Kotov et al, 2003;Pevtsova et al, 2008;Pevtsova et al, 2009;Tolpygo et al, 2012. Frontiers in Pharmacology | www.frontiersin.org November 2021 | Volume 12 | Article 772217 (Kaschina and Unger, 2003). However, the observed effects of AngII-BSA on hemodynamic parameters are much weaker when compared with AngII.…”

“…Significantly, sometimes cardinally differing spectra of the activity of free and protein-bound angiotensins were demonstrated in a number of our works (Kotov et al, 2003;Pevtsova et al, 2008;Pevtsova et al, 2009;Tolpygo et al, 2012). For the study, central effector RASoctapeptide AngII and its derivatives heptapeptide Ang (1-7) and hexapeptide AngIV were selected.…”

“… Notes: Hemodynamic effects: an increase (↑) or a decrease (↓) values are indicated, the percentage change in the measured parameters relative to the control is indicated in parentheses; behavioral effects: minus/plus means the absence/presence of an effect, the severity of which increases from minimum (+) to maximum (++++). The data were taken from Kotov et al, 2003 ; Pevtsova et al, 2008 ; Pevtsova et al, 2009 ; Tolpygo et al, 2012 . …”

“…Conjugation with a carrier protein can globally, up to functional inversion, change the properties of AngIV ( Pevtsova et al, 2009 ) which has its own receptor, insulin-regulated aminopeptidase, and plays an important role in learning and memory mechanisms ( Wright and Harding, 2019 ). Ang IV has cardioprotective effect, whereupon it is considered to be pharmacologically perspective peptide ( Hussain and Awan, 2017 ).…”

Behavioral and Hemodynamic Effects of Free and Protein-Bound Angiotensin IV in Rats in Experimental Hypo- and Hyperglycemia: Comparative Aspects

“…According to this concept, carrier proteins modulate activity of angiotensins in PPC, thus providing their differential involvement into regulation at the homeostatic and behavioral levels [1,2].…”