Complexation of spermine and spermidine by myo-inositol 1,4,5-tris(phosphate) and related compounds: biological significance

Mernissi-Arifi, K.; Imbs, Isabelle; Schlewer, Gilbert; Spiess, Bernard

doi:10.1016/0304-4165(95)00178-6

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…We suppose that this new molecular conformation can favour aggregation among the polyamines and the phosphates, with the consequent generation of NAPs. Our belief is supported by evidence from several NMR studies that demonstrated the formation of complexes among polyamines and phosphorylated molecules [31–33]. The formation of a polymeric aggregation of polyamines and phosphates can be also inferred by our spectrophotometric analyses showing that these compounds gave two absorbance peaks at 200 and 265 nm.…”

Section: Discussionsupporting

confidence: 81%

Polyamines interact with DNA as molecular aggregates

D’Agostino

Luccia

2002

European Journal of Biochemistry

103

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New compounds, named nuclear aggregates of polyamines, having a molecular mass of 8000, 4800 and < 1000 Da, were found in the nuclear extracts of several replicating cells. Their molecular structure is based on the formation of ionic bonds between polyamine ammonium and phosphate groups. The production of the 4800 Da compound, resulting from the aggregation of five or more < 1000 Da units, was increased in Caco-2 cells treated with the mitogen gastrin. Dissolving single polyamines in phosphate buffer resulted in the in vitro aggregation of polyamines with the formation of compounds with molecular masses identical to those of natural aggregates. After the interaction of the 4800 Da molecular aggregate with the genomic DNA at 37°C, both the absorbance of DNA in phosphate buffer and the DNA mobility in agarose gel increased greatly. Furthermore, these compounds were able to protect the genomic DNA from digestion by DNase I, a phosphodiesterasic endonuclease. Our data indicate that the nuclear aggregate of polyamines interacts with DNA phosphate groups and influence, more efficaciously than single polyamines, both the conformation and the protection of the DNA.

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Section: Discussionsupporting

confidence: 81%

Polyamines interact with DNA as molecular aggregates

D’Agostino

Luccia

2002

European Journal of Biochemistry

103

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“…Spermine may also, under certain conditions (low Ca 2+ concentration), inhibit PI-PLC [38,53]. The resulting IP 3 deprivation may be intensified by the binding of spermine to IP 3 [31]. The IP 3 receptor function is modified by several thiol reagents [4,16,19].…”

Section: Discussionmentioning

confidence: 99%

Spermine increases the active and passive transport across the alveolar epithelium in situ: effect of thiol reagents

Saumon

Martet

2001

Pfl�gers Archiv European Journal of Physiology

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An intact alveolar epithelial barrier is thought to be important for alveolar liquid absorption. However, polycations increase alveolar permeability without affecting alveolar liquid absorption (Saumon et al., Am J Physiol 1995: 269:L185-L194). We have reconsidered this issue using polyamines. The polyamine spermine (10(-3) mol/l) produced a large (up to 20-fold), sustained increase in the permeability of the alveolar barrier to mannitol (PAMan) and in alveolar liquid absorption (Jw, twofold) in isolated rat lungs. These increases were inhibited by 5 x 10(-3) mol/l putrescine and 2 x 10(-3) mol/l spermidine. Because spermine is known to affect the phosphoinositide/Ca2+ signalling pathway, we evaluated the effects of thiol reagents known to interfere with this pathway in different ways. Thimerosal, a thiol reagent which sensitizes the inositol 1,4,5-trisphosphate (IP3) receptor, inhibited the spermine-induced increase in PA(Man) and, to a lesser extent, that of Jw. Mersalyl, a thiol reagent which blocks IP3-gated Ca2+ channels, enhanced spermine's effect, whereas N-ethylmaleimide, a non-specific thiol reagent, had no effect. These observations show that large increases in permeability may coexist with increases in Jw. They also suggest that the phosphoinositide/Ca2+ second messenger pathway is involved in modulating the tightness of the alveolar barrier and alveolar liquid absorption.

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“…Some investigators have proposed a role for PA in the signal transduction pathways mediated by Ca 2ϩ and Ins(1, 4, 5)P 3 . In fact, PAs can interact and form stable complexes with Ins(1, 4, 5)P 3 , which undoubtedly influences the signal transduction mechanism (Mernissi-Arifi et al, 1996). In addition, PAs stimulates GTPase activity of purified G proteins, the regulatory molecules of phospholipase (PLC) activity (Bueb et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Comparative effects of TGF? on proliferation of 7- and 14-day-old chick embryo fibroblasts and lack of involvement of the ODC/PA system in the TGF? signaling pathway

et al. 1999

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The growth regulatory activity of transforming growth factor beta (TGFbeta) on chick embryo skin fibroblasts was compared in two developmental ages, days 7 and 14. The time course of 3H-thymidine incorporation, an S-phase marker of replication, was determined during 36 hr of TGFbeta treatment. Seven-day-old cells showed a prereplicative phase of 6 hr, and 14-day-old cells showed a prereplicative phase of 12 hr. DNA synthesis peaked at 24 hr in 7-day-old fibroblasts and was 10 times higher than that in 14-day-old fibroblasts. Ornithine decarboxylase (ODC) activity and content of the natural polyamines spermine (Spm), spermidine (Spd), and putrescine (Put) differed during cell cycle. ODC activity peaked at 12 hr in 7-day-old cells and at 6 hr in 14-day-old cells. Its level was two times higher at day 7 and was associated with a greater content of ODC mRNA. The maximum of polyamine (PA) concentration was determined after 12 hr of treatment in 7-day-old cells and after 36 hr in 14-day-old cells. These findings indicate that the TGFbeta proliferative response of embryo fibroblasts changes during development and is associated with activation of the ODC/PA system. Cotreatment with alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ODC, did not reduced growth rate. Inhibition of ODC resulted in levels of Put and Spd comparable to that of quiescent fibroblasts, whereas Spm concentration remained higher. Because an altered ODC metabolism does not convey the effects of TGFbeta on DNA synthesis, the ODC/PA system may not play a role in the pathway of TGFbeta signaling.

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Complexation of spermine and spermidine by myo-inositol 1,4,5-tris(phosphate) and related compounds: biological significance

Cited by 12 publications

References 40 publications

Polyamines interact with DNA as molecular aggregates

Polyamines interact with DNA as molecular aggregates

Spermine increases the active and passive transport across the alveolar epithelium in situ: effect of thiol reagents

Comparative effects of TGF? on proliferation of 7- and 14-day-old chick embryo fibroblasts and lack of involvement of the ODC/PA system in the TGF? signaling pathway

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