Complexation of pectins varying in overall charge with lysozyme in aqueous buffered solutions

Abstract

DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:

Generality and specificity of the binding behaviour of lysozyme with pectin varying in local charge density and overall charge

“…In contrast to the BP/lys system the complex phases of the SP66.5/lys and SP77.7/lys systems are liquid and have a droplet morphology (Antonov et al, 2019). Hence, the microscopy images demonstrate that another main difference between the complex particles of the highly methoxylated BP/lys and SP/lys systems is the aggregation state (liquid or solid) of the complex phase.…”

“…The qonset and q values as a function of DM for BP/lys systems are presented in Figure 2. Similar to SP (Antonov et al, 2019) BP interacts with lys starting from an extremely low weight ratio of pectin in the mixtures namely qonset =7•10 -4 for highly charged BP16.9 and ranges to 3•10 -3 for BP68. Water soluble complexes are formed in a narrow q range between 7•10 -4 and 1.4 10 -2 depending on the DM values, as shown in Figure 2.…”

“…These correspond to respectively the transition from the absence of CP to the formation of water soluble complexes, from water soluble complexes to water insoluble complexes and their phase separation, and maximal CP. To obtain accurate values for these transition points, additional characterizations were performed, as described in detail in our recent work (Antonov et al 2019).…”

“…The turbidimetry data of these systems at 500 nm (500) as a function of q are presented in Figures 1 and 2. The thin dash dot lines in Figures 1 show the data for the SP/lys system from our previous study (Antonov et al 2019) for comparison. At first glance a comparison between the data obtained for BP16.9/lys, BP33.1/lys and BP68.2/lys systems and that for SP16.2/lys, SP38.2/lys and SP66.5/lys systems studied recently shows that replacing pectin with a statistical distribution of methyl ester groups with pectins with a blockwise distribution of methylester groups at the same DM values does not appreciably affect the turbidimetry results (Fig.…”

“…In our recent study we characterized the CP of pectin with lysozyme (lys) at different physicochemical conditions and compositions of the system (Antonov et al, 2019). The effect of the overall charge of pectin (DM) on the complexation behavior was investigated.…”