Complexation of macroporous amphoteric cryogels based on N,N‐dimethylaminoethyl methacrylate and methacrylic acid with dyes, surfactant, and protein

Abstract

Macroporous amphoteric cryogels based on N,N-dimethylaminoethylmethacrylate and methacrylic acid p(DMAEM-co-MAA) crosslinked by N,N 0 -methylenebisacrylamide (MBAA) were synthesized by radical copolymerization of monomer mixtures in cryoconditions. The structure and morphology of cryogels were evaluated by FTIR and SEM. Cryogels exhibited interconnected porous structure with pore size ranging from 40 to 80 mm, which depended on their crosslinking degree. The value of the isoelectric point (IEP) of equimolar amphoteric cryogel determined from the water flux was equal to 4.4, while the IEP of cryogel with the excess of DMAEM units was equal to 7.1. The mechanical strength of equimolar amphoteric cryogels increases with increasing amount of crosslinking agent. The complexation ability of amphoteric cryogels with respect to surfactant, dyes, and protein was demonstrated. The adsorption isotherms with respect to anionic surfactant-sodium dodecylbenzene sulfonate (SDBS) and protein-lysozyme correspond to Langmuir equation, while adsorption isotherms of anionic and cationic dyes-methylene blue (MB) and methyl orange (MO) are well described by Freundlich equation. It was found that the binding ability of p(DMAEM-co-MAA) with respect to various low-and high-molecular weight compounds changes in the following order: SDBS > lysozyme MO > MB. The preferential adsorption of MB from the mixture of protein and MB was shown. The quantitative release of protein, surfactant and dye molecules from the matrix of cryogels takes place at the IEP of cryogel.

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

“…Due to their interconnected, macroporous 3D structure, cryogels have been widely used in bioseparation‐related applications [1–10], tissue engineering [10–14], and other relevant bioengineering and sensor applications [15–17]. In particular, monolithic cryogels have been used as a new generation of chromatographic matrices for the separation of cells (mammalian, bacterial, and yeast), proteins, viruses, and plasmids [18–22].…”

Flow-Through Catalytic Reactors Based on Metal Nanoparticles Immobilized within Porous Polymeric Gels and Surfaces/Hollows of Polymeric Membranes

“…The idea of the use of macroporous and supermacroporous cryogels as flow-through catalytic reactors was for the first time suggested in [12] and experimentally realized in [7,[26][27][28][29][30] for hydrogenation of nitroaromatic compounds ( Figure 7). Later on Sahiner et al [31,32] using the same principles and glass column reactor repeated reduction of 4-nitrophenol (4-NP) to corresponding 4-aminophenol (4-AP) with the help of superporous cryogels containing various metal nanoparticles and were able to lower the activation energy (E a ) of catalytic reduction of 4-NP considerably in comparison with similar studies reported in the literature.…”

“…Equimolar polyampholyte cryogel P(DMAEM-co-MAA) synthesized from acidic and basic monomers, such as N,N-dimethylaminoethylmethacrylate (DMAEM) and methacrylic acid (MAA) effectively reduces rhodium, palladium, gold and silver ions under heating conditions and forms fine well-dispersed metal nanoparticles without the use of any other reducing agents [30]. Moreover micron-sized cryogel matrix provides fast swelling during 0.5-2 min and high water flux [26].…”

Intra- and Interpolyelectrolyte Complexes of Polyampholytes

“…The feasibility of the “isoelectric effect” was tested for crosslinked amphoteric macroporous cryogels consisting of N , N -dimethylaminoethylmethacrylate and methacrylic acid (DMAEM- co -MAA) with respect to surfactant—sodium dodecylbenzene sulfonate (SDBS), dyes methylene blue (MB) and methyl orange (MO), and protein lysozyme [155]. It was found that the release efficiency of dye, surfactant and protein at the IEP from amphoteric cryogel (pH IEP = 7.1) can reach up to 98%.…”